查询词典 plasmid

According to the sequence and functional analysis result some ORFs was selected to overexpress in E. coli. Cloning of the endogenous plasmid SLP3 of S.

随后根据测序的结果以及复制区和接合转移功能区鉴定的结果，选择对一些重要的功能蛋白进行在大肠杆菌中的过量表达。

Digested by endonuclease, the linear plasmid was injected into the copulatory pouch of the female moth and the sperm reservoir of the male moth. Let them mate with the normal moth and oviposit.

将打靶质粒酶切线性化后，分别注入到雌蛾交配囊和雄蛾贮精囊，与正常蛾交配产卵，分析外源基因在当代的情况。

The green fluorescent protein (pGFP-C2) plasmid DNA was used as target gene. SDIONpGFP-C2 conjugate compounds were produced by means of oxidoreduction reaction.

采用化学共沉淀法制作SDION，通过丙烯葡聚糖凝胶S-300HR色谱和离心法分离SDION，用透射电镜、粒度分析仪和磁力计对SDION进行分析。

The successes in connecting SDION with pGFP-C2 plasmid by means of oxidoreduction reaction and in transferring pGFP-C2 gene into human bladder cancer BIU-87 cells in vitro provided the experimental evidence for the feasibility of SDION used as a novel gene carrier.

SDION可通过氧化还原反应与GFP质粒相连，在体外可将GFP基因成功转染入人膀胱癌BIU-87细胞。

The recombinant vector was constructed by subcloning SV40LT gene (2.1 Kb) from SV40LT gene-carrying plasmid pLLTSN into the BamH I and Xho I sites of pantropic retroviral expression vector pLXRN.

从携带SV40LT基因的质粒pLLTSN中高保真PCR扩增出2.1 Kb的SV40LT基因，将其插入到泛嗜性逆转录病毒载体pLXRN的BamHⅠ和XhoⅠ位点之间，构建重组载体pLXRN-SV40LT。

Plasmid DNA of pBILGC containing Chitinase and β-1, 3 - glucanase gene and fresh pollen of Papaver nudicaule were mixed in 11％ sucrose solution, then the pollen suspension were pollinated on stigmas of the Papaver nudicaule by artificial pollination.

以野罂粟开放花蕾为受体，以载有几丁质酶和β-1，3-葡聚糖酶基因双价植物表达载体pBLGC的质粒为供体，在11％浓度的蔗糖溶液中，加入花粉和含有目的基因的质粒DNA。得到药粉处理液。

The soluble human tumor necrosis factor ; human tumor necrosis factor ; plasmid ; pericementum cell ; tranfected

可溶性肿瘤坏死因子；肿瘤坏死因子；质粒；牙周膜细胞；转染

Recombining the plasmid PcDNA3.1-sTNFRⅠ.sTNFRⅠin cell culture supernatant was detected by ELISA and sTNFRⅠblockage of TNFαin pericementum cell by MTT assay.

RT-PCR方法筛选目的基因，并构建质粒；ELISA方法检测牙周膜细胞转染PcDNA3.1-sTNFRⅠ后培养上清中目的基因的表达；MTT检测目的产物是否在牙周膜细胞中有抑制TNF-α的作用。

Microbubbles attached with the naked plasmid DNA of EGFP were infused into the rat by periocular injection with destructing microbubbles by ultrasound immediately. After 2 weeks , the EGFP expression in the rats'retina were detected by confocal laser scanning microscopy .

两周后处死大鼠做冰冻切片，在激光共聚焦显微镜下观察蛋白表达情况及分析EGFP质粒在大鼠色素上皮层的表达强度。

For example, it is a pathogenic bacterium and has endotoxins; it secretes protein into the periplasm and often into inclusion bodies. Bacillus subtilis is an attractive host for the production of heterologous secretory proteins. Many genes has succeed expressed in Bacillus subtilis, and the plasmid vectors for Bacillus subtilis are now taken from other genus bacterium, such as Staphylococcus aureus or lactococcus lactis and so on. As it is clear to known that these plasmids are far from optimal for Bacillus subtilis, because of low efficiencies of cloning and high levels of replicating instability.

枯草芽胞杆菌是目前原核表达系统中分泌表达外源蛋白较理想的宿主，近几年已在枯草芽胞杆菌中成功表达了多种基因，但大多都应用来源于金黄色葡萄球菌、乳酸杆菌等的载体来表达外源基因，由于其在枯草芽胞杆菌中存在分离复制的不稳定及外源基因的克隆表达效率低的现象，故都不是枯草芽胞杆菌的最佳选择，还有待改进。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！