查询词典 plasmid

Plasmid pO26-CRL is nonconjugative but is mobilizable.

质粒pO26-CRL是非结合转移类的，但它具有可移动性。

At the same time, it can enhance utilization rate of phosphorus for monogastric animals and solve environmental problems as well.Three linearized DNA fragments without selectable marker gene and vector backbone were constructed containing phyA gene with regulation sequences or T-DNA borders or the plasmid borders.

我们构建了仅含有启动子序列、植酸酶基因和终止子序列的线性基因转化元件及其两端分别带有T-DNA边界序列或一段载体序列的不同线性转化元件，不含选择性标记基因和载体骨架序列，采用花粉管通道法对选定的玉米品种进行转化，共计收获的可以检测的种子为20400粒。

Methods Expression plasmid was constructed with DNA recombi nant technique.

方法采用DNA重组技术构建结核分支杆菌Ag85B基因的表达载体，双酶切和聚合酶链反应鉴定重组子，阳性重组子转化大肠杆菌，并诱导表达外源蛋白。

One could nodulate the soybean cultivars tested and showed wider host range, the other only nodulate limited soybean cultivars. Plasmid could not be detected from twenty-three slow-growing strains and twenty-nine fast-growing strains were found containing 1-4 plasmids by Echardts method.

质粒快速检测结果表明：慢生型大豆根瘤菌均不含有质粒，快生型大豆根瘤菌均有1-4个质粒，质粒类型与宿主亲和性也有一定的相关性。

The resulting strain, designated YES2MTM1, was transformed with a yeast genomic library. Transformants lost the plasmid overexpressing MTM1 after 5-FOA treatment. Yeast strains able to grow on nonfermentable carbon source with MTM1 deletion and overexpression of some DNA fragments were picked up and candidate suppressor genes were identified. Overexpression of five genes were identified to be able to rescue the growth defect on nonfermentable carbon source. The study will provide reference for MTM1 gene function and screening for suppressor of genes whose deletion result in irreversible damage.

为了避免MTM1缺失造成的不可逆损伤，在野生型酵母中先转入带有MTM1 基因的质粒，再敲除染色体上的MTM1 基因，随后转入基因组文库，再利用药物5-氟乳清酸(5-FOA)迫使细胞丢失表达MTM1基因的外源质粒，再筛选能在非发酵培养基上生长的转化子，通过这种方法筛选发现，POR2等5个基因的过表达可以挽救MTM1 基因缺失造成的非发酵培养基上的生长缺陷，为深入了解MTM1基因的功能提供了线索，对筛选其他造成不可逆损伤的突变基因的抑制基因提供了一条可行的研究思路。

Then we transformed mTn-lacZ/LEU2 transposon library into the YES2MTM1 strain. Transformants lost the plasmid overexpressing MTM1 after 5-Fluoroorotic acid treatment. We picked up the yeast strains able to grow on nonfermentable carbon source with MTM1 deletion and some transposon insertion and identified the insertion sites.

因MTM1基因的缺失造成的损伤不可逆，直接转入文库无法筛选得到MTM1 基因缺失表型相关基因，本研究利用外源MTM1基因菌株和mTn-lacZ/LEU2酿酒酵母转座子文库进行筛选，寻找能挽救mtm1突变体生长缺陷的转座子插入位点。

Although octopine synthase and mannopine synthase genes initally resident on Ti plasmid of Agrobacterium tumefaciens, they possess all of the sequence elements required for transcription in plants.

甘露碱合成酶基因启动子和章鱼碱合成酶基因启动子虽然来源于农杆菌Ti质粒，但却具有植物启动子的特性，是目前使用广泛的两个组成型启动子。

College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,ChinaAbstract: To obtain the hybridoma cell line specific to 45W-4BX antigen of Taenia solium oncosphere,the recombinant plasmid pGEX-4BX containing TSO45W-4BX gene was highly expressed in Escherichia coli induced with IPTG.

为获得针对猪带绦虫六钩蚴45W-4BX抗原的杂交瘤细胞株，用IPTG对TSO45W-4BX的重组质粒pGEX-4BX进行了诱导表达，并采用Sepharose-4B层析技术对表达产物进行纯化，运用SDS-PAGE和Western-blot分别对其纯度和活性进行了检测。

Eg95 antigen cDNAs were amplified by PCR from protoscolex, oncosphere and adult worm cDNA libraries of E. granulosus, respectively and were cloned into pUCm-T plasmid, and sequenced. The sequences were analyzed by DNAman and GenBank/BLAST biosoftware.

方法根据Eg95基因序列设计引物，用PCR方法分别从新疆株细粒棘球绦虫3个不同发育阶段所构建的cDNA文库中克隆获得Eg95目的基因，将其克隆至pUCm-T载体、测序并进行序列分析。

The Taenia solium oncosphere 18 gene was cloned and reconstructed into expression vector pYA3341.The recombinant plasmid pYA3341-TSOL18 was finally electro-transformed into an attenuated S.typhimurium definitive host strain of X4500 and the recombinant strain of X4500 (pYA3341-TSOL18) was identified its immunogenicity of expression protein, stability, growth curve, safety in vitro and immunity evaluation by animal experiment in mouse.

克隆并改造TSOL18基因，构建重组质粒pYA3341-TSOL18，电转入鼠伤寒沙门氏菌终宿主菌株X4550，体外鉴定重组菌X4550(pYA3341-TSOL18)表达蛋白的免疫原性、稳定性、生长曲线、安全性和小鼠免疫试验进行评价。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！