查询词典 plasmid

A full-length cDNA encoding sucrose: sucrose 1-fructosyltransferase from Lactuca sativa was inserted into pCAMBIA1300-als under the control of the CaMV 35S promoter. This plasmid was used for Agrobacteriummediated tobacco leaf disc transformation. Transgenic plants were analyzed by PCR and Southern blotting. 1-SST gene expression was confirmed by RT-PCR.

将从莴苣中克隆的1-SST基因重组到pCAMBIA1300-als中，构建了在CaMV 35S启动子调控下的植物表达载体，利用农杆菌介导的叶盘转化法将1-SST基因导入烟草中，PCR和Southern杂交检测表明获得了转基因植株，RT-PCR结果表明该基因在烟草中正常表达。

E. coli cells harboring the recombinant plasmid was cultured with Laria broth medium and induced with isopropyl β-D-thiogalactopyranoside and detected from supernatant and precipitate of culture lysates by using SDS-PAGE.

将重组菌培养并经IPTG诱导后用超声波裂解菌体，取上清和沉淀同时作SDS-PAGE电泳后显示，上清中没有热休克蛋白A的表达，在沉淀中有热休克蛋白A的表达。

By the bioinformatics analyzing tools in bioinformatics webs site such as NCBI （http:///www.ncbi.nlm.nih.gov/）, ExPaSy （http://www.expasy.org/） and combining else complicated bioinformatics software package ,to identify a EF-1 full-length gene from the Taenia asiatica eDNA plasmid libratory and predict the structure and function of the protein.

方法利用生物信息网站如美国国家生物技术信息中心（NCBI，http：∥www.ncbi.nlm.nih.gov/）和瑞士生物信息学研究所的蛋白分析专家系统（ExPASY，http：∥ca.expasy.org/）中有关基因和蛋白的序列和结构信息分析的各种工具，结合其它生物信息学分析软件包，从亚洲带绦虫成虫全长cDNA质粒文库中识别延伸因子-1的全长编码基因并预测其蛋白的结构和功能。

Methods By the bioinformatics analyzing tools in bioinformatics webs site,a Ta CRISP 2 fulllength gene from the Taenia saginata asiatica fulllength cDNA plasmid libratory was identified and the coding region sequence and the characteristics of the deduced protein were analyzed.The coding region of Ta CRISP was amplified with PCR,endonuclease digestion and cloned into the prokaryotic expression vector pET30a and then expressed in E.coli BL21 with IPTG induction.

用生物信息学方法从亚洲牛带绦虫成虫全长cDNA质粒文库中识别TaCRISP的全长编码基因并预测编码蛋白质的各种结构与功能；将其编码区序列克隆到原核表达载体pET-30a上，测序鉴定重组质粒，之后进行诱导表达，表达产物经十二烷基磺酸钠-聚丙烯酰胺凝胶电泳鉴定。

Coll. Methods Insert the synthetic SARS-CoV N protein gene into vector pUC-18,ligate to plasmid pET28 and transform to E.

方法将已合成的SARS-CoV N蛋白基因片段，克隆至pUG18载体，与pET28质粒连接，转化大肠杆菌，进行SARS-CoV核衣壳融合蛋白表达。

The results showed that expression plasmid pET22b-lysB was constructed successfully. Highly purified recombination protein was obtained 33.2 mg from 1 L LB culture medium. A screening for His-LysB activity on esterase and lipase substrates confirmed the lipolytic activity. With p-nitrophenyl butyrate as substrate, the thermal stability of the enzyme was poor when the temperature was above 30oC. The enzyme exhibited higher stability at pH 5.0–9.5. The optimum temperature and pH for the lipolytic activity of His-LysB were 23oC and 7.5 respectively. Under the optimum conditions, the specific activity of His-LysB was 1.3 U/mg. Zn2+, Cu2+, Mg2+, Mn2+and phenylmethane sulfonyl fruoride severely inhibited the lipolytic activity of His-LysB.

结果表明：成功构建了pET22b-lysB表达载体，并从1 L的LB培养物中获得了33.2 mg高纯度重组蛋白；His-LysB具有分解脂肪的能力，属于脂肪酶；生物化学特性分析表明：丁酸对硝基苯为水解底物，His-Lys热稳定性不佳，30℃以下比较稳定，随着温度的升高，稳定性逐渐降低；该蛋白具有较高的pH值适应性，pH 5.0~9.5范围内稳定性较高；在23℃和pH 7.5时酶活力最高，其比酶活为1.3 U/mg；金属离子Zn2+、Cu2+、Mg2+、Mn2+和苯甲基磺酰氟抑制剂对酶活具有强烈的抑制作用。

We first constructed the CAT and Luc reporter plasmid deleting CRE or AP-1 site or both.

首先构建了CRE和AP-1位点系列缺失的CAT和Luc报告基因质粒。

A GARP (glutamic acid-rich protein) gene was isolated from the macronuclear plasmid mini library of Euplotes octocarinatus .

一种富含GAA三核苷酸的 GARP (glutamic acid-rich protein)基因从八肋游仆虫大核文库中筛选获得。

With the method of reverse transcription-polymerase chain reaction and nest PCR, P80 gene of CSFV is cloned from the cells infected by the strain Shimen of csfv, and connected with eukaryotic expression vector pEGFP-C1. Then recombined eukaryotic expression plasmid pEGFP-P80 of P80 gene is achieved successfully. It builds the basis for expressing and gaining protein p80 in mammiferous cells.

利用反转录PCR和套式PCR技术，从猪瘟病毒Shimen株细胞毒中克隆出P80 基因，并进一步把P80 基因连接在真核表达载体pEGFP-C1 上，成功地构建出P80 基因重组真核表达质粒(pEGFP-P80)，为在哺乳动物细胞中表达并纯化出p80 蛋白奠定了基础。

New plasmid-mediated quinolone resistance gene, qnrC, found in a clinical isolate of Proteus mirabilis.

qnrC，一种从奇异变形杆菌分离得到的新型的质粒介导的喹诺酮抵抗基因

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！