查询词典 plasmid

The N gene ORF was then subcloned into pET-30a vector and the recombinant plasmid was transformed into E.coli BL21 (DE3) and induced with IPTG. The protein expression was determined by SDS-PAGE. The expressed protein had a molecular weight of 54.4 Ku that existed as inclusion body. Thin-layer scanning showed that the expression product accounted for 30.5% of the total bacterial proteins. The recombinant protein possessed native biological activity and could react with anti-PEDV hyperimmune serum in Western blot.

以阳性质粒为模板，用分别含有BamH Ⅰ和Xho Ⅰ酶切位点的上、下游引物扩增得到ORF，其PCR产物经BamH Ⅰ和Xho Ⅰ双酶切后定向克隆到pET-30a载体，构建的重组质粒命名为pET-30a-PN；将pET-30a-PN转化到大肠杆菌BL21 (DF3)中，在IPTG诱导下进行表达；SDS-PAGE结果表明表达出与预期大小相符的约54.4 Ku的重组蛋白，重组蛋白以包涵体形式存在；薄层扫描结果表明表达产物占菌体总蛋白的30.5%；Western blot分析表明表达的重组蛋白能与抗PEDV高免血清反应，说明该重组蛋白具有免疫学活性。

In conclusion, a prokaryotic plasmid, encoding the fusion gene of single-chain variable fragment with MCP-3 and expressing idiotype protein vaccination against B cell lymphoma, was constructed correctly.

本研究成功构建鼠源scFv片段与趋化因子MCP-3融合的独特型B细胞淋巴瘤疫苗表达质粒pGLo/scFv- MCP-3，并实现融合蛋白的初步表达。

An immunogenically active component of West Nile virus or plasmid DNA, an adjuvant such as a metabolizable oil, and a pharmacologically acceptable carrier are formulated into an immunizing vaccine.

将一种西尼罗河病毒或质粒DNA的免疫原性活性成分、一种诸如可代谢油的佐剂和一种药学可接受载体共同配制成一种免疫疫苗。

We construct pCDNA3.1-TfRexpression plasmid successfully, there are higher expression of TfR aftertransient and stable transfection; we get positive cell clone of containingpCDNA3.1-TfR, establish infarctate foundation to empirical study ofmolecular imaging of MR.

成功构建了pCDNA3.1-TfR表达质粒；质粒转染U251细胞后瞬时与稳定表达的TfR蛋白明显增高；成功筛选出pCDNA3.1-TfR阳性细胞克隆，为分子影像学的实验研究奠定了坚实的基础。

In order to analyze proteins which may interact with AIF, interceptive AIF gene (mitochondrial localization sequence, MLS, was deleted) which was amplified from the total mRNA extracted from hepatoma HepG2 cells by reverse transcriptase polymerase chain reaction, was inserted into pcDNA3.1 plasmid.

为了筛选可能与截断型AIF存在相互作用的蛋白质，探讨截断型AIF诱导细胞凋亡的机制，用逆转录PCR扩增剪切掉线粒体定位信号的人AIF基因片段并克隆入pcDNA3.1载体，导入肝癌HepG2细胞。

Methods: After transferred pcDNA3.1/hTM plasmid into rabbit artery by high-pressure injection, rabbit common iliac artery were cut and anastomosed again. At the 14 days and 28days after second operation, we checked inside diameter of anastomotic stoma and blood flow velocity by color Doppler. The treated artery were sliced and stained by Verhoeff. Local neointima formation and the ratio stenosis of intervascular were calculated by computer.

用注射式加压转染的方式对兔动脉壁转染pcDNA3.1/hTM质粒，再制造动脉损伤-阻滞模型，于术后14天、28天用彩色多普勒观察活体吻合口内径和血流流速；再做病理切片Verhoeff染色，观察血管内膜增生的程度、部位，计算血管内膜面积、中膜面积和血管狭窄率。

BALB/c mice were injected with the recombinant pCMV-ME plasmid DNA contained PrM-E gene interdermally and intramuscularly.

在证明其可在哺乳动物细胞中高效表达的基础上，进一步用重组质粒DNA免疫小鼠。

BALB/c mice were injected with the recombinant pCMV-ME plasmid DNA contained PrM-E gene interdermally and intramuscularly.

在证明其可在哺乳动物男性细胞中高效表达的基础上，进一步用重组质粒DNA免疫小鼠。

Methods : The eukaryotic expression plasmid containing GRP78 gene was injected intramuscularly into hind leg of C57BL/6 female mice.

将携带GRP78基因的真核表达载体肌肉内注射免疫C57

The experimntal results showed that the recombinant plasmid could be expressed in the COS-7 cells with a typical T-helper 1-dominanted immune response as demonstrated by IgG isotype analysis.

结果 扩增的BCSP31保护性抗原基因片断在牛、羊和猪布氏杆菌株中具有高度保守性，构建的BCSP31/pVAX1重组表达质粒在COS7细胞有目的蛋白抗原的表达。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！