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Objective: To contructre combinant plasmid pET28a/hES and coexpress human Endostatin and predhPK-5 in E. coli. Methods: mRNA from human liver tissue was exracted, and the Endostatin gene was amplified by RT-PCR.

目的构建人内皮抑素原核表达质粒pET28a/hES，并与简化人纤溶酶原饼环区(predigested human PlasminogenKringle5，predhPK-5)在大肠杆菌中实现共表达。

Recombinant adenovirus was identified by polymerase chain reColon cancer cell line SW480 was infected with recombinant adenovirus Ad-p27mt, and expression of p27 protein was detected by Western blot.of expressed p27 protein after Ad-p27mt transfected colon cancer cell.novirus framework plasmid pAdeasy-1 with pShuttle-CMV-hp27mt, 30％combinant adenovirus DNA contained the target gene.

Ad-p27mt的聚合酶链反应检测Ad-p27mt转染大肠癌细胞后的p27mt的表达。结果：①用含pShuttle-CMV-hp27mt转化含pAdeasy-1的超感受态BJ5183后，可获得约30％的阳性重组质粒。②聚合酶链反应检测表明重组腺病毒DNA中含有目的基因。重组腺病毒滴度为7.95×1015pfu/L。

The GPA1 gene was obtained via PCR amplification and was cloned in the two-hybrid DNA binding domain vector pGBKT7 The combinant plasmid was designated as pGBKT7-Ga.β-galactosidase assay indicatedthat Got did not have the property of self-activation. After pGBKT7-Ga was transformed to yeast pJ69-4A, we transformed Arabidopsis vegetative tissue two-hybrid cDNA library plasmids to yeast pJ69-4A containing pGBKT7-Ga.

通过PCR扩增得到GPA1基因并将其克隆到双杂交DNA结合域载体pGBKT7中，得到的重组质粒命名为pGBKT7-Gα，β—半乳糖苷酶活性鉴定表明Gα不具有自激活特性，将其转化到酵母菌pJ69-4A中，再以此为受体菌转化拟南芥绿色营养组织cDNA文库质粒。

With the comparition of the two groups, the conclusion can be drawn that the p80 protein expressed by the plasmid pEGFP-P80 cannot cause the detectable depression of activity of PK-15 cells.

结果表明，利用pEGFP-P80 重组表达质粒在PK-15 细胞中表达出的p80 蛋白未对宿主细胞有明显的致病变作用。

The result suggests that Al2O3 nanoparticle can stimulate donor function and thus result in a significant increase in conjugative plasmid dissemination in aquatic environments.

在一定条件下，纳米材料可促进质粒在液相中的接合转移，从而可能引发纳米材料的水环境安全问题。

Methods Agarose gel electrophoresis analysis was used to detect plasmid profiles. Plate transconjugation and broth transconjugation were used to examine the conjugative resistant plasmids and the agar plate dilution method was used to analyze the minimum inhibitory concentration of 15 antimicrobial agents.

用改良Kado-Liu法提取细菌质粒，琼脂糖凝胶电泳检测质粒，用平板稀释法测定药物最低抑菌浓度，用试管内肉汤接合法和平板表面接合法进行接合转移试验。

In order to do this, we get equal quantities of plasmid pSCA1-M, pcDNAM and pSCA1 transfected vero cells. After 48 hours, we observed the presence of special green fluorescence in cytolymph in both of pSCA1-M and pcDNAM groups. However, this fluorescence is absent in the negative pSCA1 group.

分别取等量的重组质粒pSCA1-M、常规IBV M基因DNA疫苗质粒pcDNAM、空载体pSCA1转染Veto细胞。48h后经间接免疫荧光试验观察发现，pSCA1-M以及pcDNAM转染组细胞浆均可见特异性亮绿色荧光，pSCA1空载体组无荧光为阴性。

Accordingly, a new plasmid Cm1301 was constructed containing osGP fragment (-197～-56) from the osRACD promoter with the minimal promoter,-90 region of CaMV 35S which confers Gus activity detectably only in roots. In the transgenic plants containing Cm1301, GUS activity was detected mostly in the anther.

由于35S基本启动子是一个根特异表达的启动子，因此将包含osDP5区段的osGP片段（-197～-56）与35S基本启动子连接，构成植物表达载体Cm-1301并转化烟草，研究发现，osGP区段使gus基因在雄蕊中表达水平显著提高。

The linear plasmid and the designed fragment were directively ligated with T4 DNA ligase,and the plant expression vector of carrot afp was constructed.

将目的片段与线性质粒在T4 DNA连接酶的作用下进行定向连接，构建成胡萝卜afp的植物表达载体pBAF。

Ten transformants and 10 conjugons showed high-level resistance against aminoglycoside antibiotics,all of which the value of MIC＞256μg/mL carried armA gene.Conclusions:The Ab clinical isolates from burn ward in Gansu Province People\'s hospital have high drug-resistance.16S rRNA methylases gene exsist in Ab and locate in plasmid chromosome.They are easily disseminative,which demonstrated a very high level of resistance to various aminoglycosides.

甘肃省人民医院烧伤病房鲍氏不动杆菌临床分离株有非常高的耐药率，16S rRNA甲基化酶基因armA在鲍氏不动杆菌中存在，且位于质粒染色体上，易于传播和扩散，导致所有氨基糖苷类抗菌药物高水平耐药。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。