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In accordance with the present invention, optically pure-hydroxycarboxylic acid can be prepared by culturing E. coli transformed with a recombinant plasmid which expresses intracellular PHA depolymerase of Ralstonia eutropha and PHA biosynthesis enzyme of Alcaligenes latus to give-hydroxycarboxylic acid.

根据本发明，光学纯的-羟基羧酸可通过培养用重组质粒转化的E.coli制备，所述重组质粒表达真养产碱杆菌的胞内PHA解聚酶和广泛产碱菌的PHA生物合成酶从而生成-羟基羧酸。

The pcDNA〓-apoE〓 plasmid was transfected to SK-N-SH Neuroblastoma cell by liposome. It could be screened by G〓. The immunohistochemical assay was shown that neuroblastoma cell transfected by pcDNA〓-apoE〓 expressed the recombinant apoE protein. The intracellular expressed recombinant protein could inhibit the death of neuroblastoma cell induced by beta amyloidal peptides 25-35 fragment.

用pcDNA〓—apoE〓真核表达载体与脂质体共转染神经成纤维胶质瘤细胞，用G〓选择压力筛选，细胞免疫荧光检测表明，pcDNA〓—apoE〓转染的神经成纤维胶质瘤细胞表达了apoE〓重组蛋白，这种内源性的apoE〓重组蛋白对25μM Aβ25—35多肽诱导的神经成纤维胶质瘤细胞的死亡具有拮抗作用。

After anesthetization of rabbits in gene groups, recombinant plasmid DNA combined with transforming growth factor-β1 (TGF-β1) was injected to C2-3, C3-4 and C4-5 intervertebral disc (20μL per intervetebral disk).

基因治疗组家兔麻醉后，将带有转化生长因子β1基因的重组质粒DNA注入C2～3，C3-4，C4-5椎间盘中(每个椎间盘用量为20μL)。

Part Ⅲ: Animal immunization and anticarious experiment of the glucan binding protein B Plasmid pcDNA3. 1 -gbpB DNA vaccine was delivered into Sprague-Dawley rats in two different routes: intranasal and targeted salivary gland immunization . The dynamic variety of specific antibodies in sera and saliva were checked by ELISA.

第三部分 DNA防龋疫苗免疫动物及抑龋实验研究大量制各重组质粒pcDNA3.1-gbpB，分鼻腔滴注组、颌下腺区皮下注射组免疫SD大鼠，并设对照组和空白组，采用ELISA法检测血清及唾液中抗体水平的变化情况。

A malignant transformant line 3T3 (T24) was obtained by transfection of mouse fibroblast NIH3T3 cells with pMAMneo-T24-ras plasmid. 3T3 (T24) cells were cultured continuously in the presence of benzamide , an NAD site inhibitor of poly polymerase .

将12位密码子突变的活化ras癌基因全cDNA序列克隆到真核表达载体pSV2neo和pMAMneo上，获得了相应的重组质粒pSV2neo-T24-ras和pMAMneo-T24-ras，将pMAMneo-T24-ras质粒利用基因转移方法导入小鼠成纤维细胞NIH3T3中，活化的ras癌基因可使受体细胞发生恶性转化。

Firstly, expression vectors harboring sense and antisense gene were constructed in pJR1 plasmid in which BoAP1 and BoCAL cDNA were inserted between the CAMV35S promoter and NOS-3'terminator.

首先构建了BoCAL、BoAP1基因的正义与反义表达载体：具卡那霉素抗性的植物表达载体pJR1--BoAP1、pJR1--BoCAL和具潮霉素抗性的植物表达载体pCAMBIA--BoAP1、pCAMBIA--BoCAL。

Borrelia burgdorferi Recombinant plasmid DNA sequence analysis Expression

姆病螺旋体；重组质粒； DNA序列分析；诱导表达

Methods We used the automatic DNA sequencing machine (Model 377) to detect the nucleotide sequence of the inserted part of the recombinant plasmid pBX1 from Borrelia burgdorferi B31 strain.

方法采用377型DNA自动测序仪对莱姆病螺旋体重组质粒pBX1的插入片段进行DNA序列测定，并通过计算机软件对其进行限制性内切酶酶谱分析。

The caprine IL-2 gene and the IFN-γgene without the signal peptidesequence were subcloned into (5") Bam H I and (3") HindⅢrestriction sites of pET30aplasmid, the recombinant plasmid were transformed into E. coli BL21(DE3) and induced withIPTG, they were demonstrated by SDS-PAGE that the genes were expressed as inclusion bodies.

应用DNA重组技术，将IL-2基因和去除信号肽的IFN-γ基因分别插入到原核表达载体pET-30a的BamHⅠ和HindⅢ位点上，构建原核表达质粒pET30a-CapIL-2和pET30a-CapIFN-γ，转化大肠杆菌BL21(DE3)，经IPTG诱导后进行SDS-PAGE电泳，结果表明目的基因在大肠杆菌中以包涵体形式融合表达。

Cavia cobaya was vaccinated with pVAX1- hsp65 and pVAX1- hsp65-10 recombinant plasmid,moreover,pVAX 1 and supersonic antigen of MAP as control.

以pVAX1-hsp65和pVAX1-hsp65-10免疫豚鼠，同时设pVAX1和副结核分枝杆菌超声抗原对照组。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！