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Delivery of a plasmid vector expressing math1 to neonatal organ of Corti cultures produced supernumerary hair cells in vitro.

在一些动物模型中如豚鼠、大鼠以及灰鼠等，能够诱导前庭毛细胞的再生。

Objective To analyze and predict the structural characteristics of Taenia asiatica elongation factor 1（EF-1） gene from adult eDNA plasmid libratory.

目的分析和预测亚洲带绦虫成虫延伸因子-1（elongation factor 1，EF-1）基因及其编码的蛋白的结构和功能。

Those expression plasmid were respectively transformed into E.coli DH5a, thenthe expression strains were induced for 5h under 42℃, SDS-PAGE confirmed that interestprotein was expressed by pBV220-R- IFN-γ_m strain and was 16kd in size. Its contentaccounted for 38.6％of the thalline protein.

分别转化大肠杆菌DH5α，42℃诱导表达4h，经SDS-PAGE电泳鉴定，原核表达质粒pBV220-R-IFN-γ_m表达的目的蛋白大小为16Kd，占细菌总蛋白的38.6％。

Aim To elusidate the effect of tissue-type plasminogen activator gene plasmid on prevention of the thrombosis after atherosclerosis.

目的 探讨组织型纤溶酶原激活物基因质粒在预防动脉粥样硬化及血栓形成中的作用。

Methods The expression plasmid pSVvWF containing full-length cDNA of vWF was used to site direct mutagenesis by polymerase chain reaction and transitorily expressed in the COS-7 cells, vWF:Ag in the supernatant and the cell lysate between wild type and pSVAla737Glu vWF were measured.

用聚合酶链反应的方法对野生型的vWF全长表达质粒进行体外定点诱变，在COS-7细胞中进行表达。

Objective To study the genetic stability of recombinant plasmid pEGFP-C1-GnRH/TRS in HeLa cells.

目的 研究质粒pEGFP-C1-GnRH/TRS在HeLa细胞中的遗传稳定性。

Recombinant plasmid pEGFP-C1-GnRH/TRS showed good genetic stability in HeLa cells.

质粒pEGFP-G1-GnRH/TRS在HeLa细胞中有较好的遗传稳定性。

Methods:The whole mature protein coding sequence of three truncated dystrophin gene was amplified by RT-PCR method applied to human muscle cDNA. The fragment was inserted into prokaryotic expression vector pET28a plasmid.

以人肌肉组织cDNA为模板，采用RT-PCR扩增三个截短的肌营养不良蛋白cDNA，分别克隆入原核表达载体pET28a中，经限制性内切酶双酶切及DNA序列分析鉴定目的基因。

In order to avoid prolin residue inhibiting enterokinase cleavage, 9bp of MGF cDNA 5′ end sequence was truncated by primer, then the obtained truncated MGF des(1-3MGF cDNA (321 bp) was subcloned in pET32a vector to construct a prokaryotic recombination expression plasmid.

通过RT-PCR从拉伸刺激的人成骨细胞中克隆MGF cDNA序列，并去除5'端9 bp的序列，使N端缺少对肠激酶(Enterokinase， EK)具有抑制作用的脯氨酸，将截短型MGF des(1-3 MGF cDNA序列克隆入pET32a质粒，构建重组表达质粒。

Methods The HPV16E7 gene was cut to three parts, amplified by PCR, and connected individually with BPVL1 on plasmid PUC. With PVL1393 as a transfect vector, the BPVL1/HPV16E7 recombinants were transfected to baculovirus which subsequently expressed the chimeric BPVL1/HPV16E7 protein in the SF－9 cells. The recombinant proteins were then purified by centrifuge, sonication, sucrose and CsCl ultracentrifuge, and were identified by SDS-PAGE, Western blot, ECL and TEM.

HPV16E7基因分3段经PCR扩增后分别克隆入连有BPVL1的质粒PUC形成BPVL1-HPV16E7；以质粒PVL1393为载体将BPVL1-HPV16E7基因转染杆状病毒并在昆虫细胞中进行表达；用超声粉碎和蔗糖超离、氯化铯梯度离心等方法纯化以及免疫印迹、ECL、透射电镜等方法鉴定表达产物。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。