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In order to develop a safe and effective immunoadjuvant to enhance the immunity and resistance of animals against infection, a novel CpG Oligodeoxynucleotides containing 11 CpG motifs was synthesized and inserted into the VR1012 plasmid, designated as VR1C. Then the recombinant VR1C was entrapped with Chitosan nanoparticles prepared by the method of ionic cross linkage, and employed to inject muscularly 3-weeks old Kunming mice; the blank VR1012 packed with CNP and saline were used to inoculate mice as the control groups. 28 days after inoculation, all mice were orally fed with 0.4ml 2x108CFU/ per mouse virulent hemorrhagic enteritis E. coli to challenge the resistance against infection.

为研制安全高效免疫调节剂增强动物免疫抗病能力，本实验设计合成含11个C pG基序的寡聚核苷酸，重组构建含CpG的VR1012质粒(VR1C)；制备壳聚糖纳米颗粒包裹重组质粒(CNP-VR1C)，肌注接种3周龄昆明小白鼠，设壳聚糖包裹空质粒和生理盐水对照组；接种后28天口服大肠杆菌攻毒观察小鼠天然免疫的变化和对强毒感染的抵抗力，Sandwich ELISA测定血清免疫球蛋白和白细胞介素含量。

AIM: To establish recombination plasmid pEGFP-NGB and to investigate the expression of pEGFP-NGB in culture neuroglia cells.

目的：构建重组小鼠质粒pEGFP-NGB并研究其在培养神经胶质细胞中的表达。

Objective To study the protective effect of various molecular weights chitosan on plasmid DNA by surface plasmon resonance technique, in order to prevent from nuclease degrading.

目的 研究采用表面等离子共振技术，检测不同相对分子质量壳聚糖对质粒DNA的保护作用，以防止核酸酶的降解。

An F plasmid may integrate into the nucleoid of the recipient strain.

F质粒可整合入受体菌的拟核。

Nucleonics employs an expressed interfering RNA approach whereby scientists insert plasmid DNA coding for relevant short hairpin RNAs into targeted cells, inducing the cells to produce and deliver specific shRNA sequences.

nucleonics公司表示，分子干扰核糖核酸方法方法，科学家插入质粒编码有关短发夹核糖核酸植入目标细胞中，让细胞产生并传递特定的shrna序列。

The nucleoprotein gene and glycoprotein gene of rabies virus were amplified by RT-PCR and then cloned into pMD18-T plasmid,respectively.

将N基因和G基因分别克隆入质粒载体pMD18-T后，对筛选的含有N基因和G基因的重组质粒进行了全基因序列测定和分析。

Objective To construct a recombinant plasmid for co-expression of nucleoprotein and glycoprotein of rabies virus aG strain in CHO-K1 cells.

目的 构建狂犬病毒aG株核蛋白和糖蛋白双表达重组质粒，在中国仓鼠卵巢细胞(CHO-K1)中表达核蛋白和糖蛋白。

In our experiment, the GADes gene from rat brain was cloned and expressed in E.coli, so as to make the recombinant GADes produced by gene technology for clinical application and further study on the relation between GAD and type 1 diabetes mellitus.Firstly, with the pUClS/GADes recombinant plasmid as template, the full-length encoding sequence of GADes was amplified by PCR, and cloned into pGEM-T vector. Secondly, the target gene was cut by EcoRI and inserted into restriction sites of pET42a vector for expression.

本研究首先用PCR方法，以pUC18/GAD_(65)为模板，扩增目的基因，克隆到pGEM-T载体中，构建pGEM-T/GAD_(65)重组质粒，然后用EcoRI酶切，切下的目的基因片段插入pET42a融合型表达载体中，经酶切鉴定和测序，证实插入方向、读码框架正确；重组表达质粒转化大肠杆菌BL21(DE_3)中诱导表达，表达产物经亲和层析纯化后获得了融合蛋白GST-GAD_(65)。

The PCR product was cloned into PGEM-T-Easy DNA plasmid by TA cloning, and hINS gene was analyst and compared to the published sequence ,and found that this hINS gene is same to one of the published sequence and there are four different bases with the other published sequence.

为了进一步验证扩增出的hINS基因能否在蛋白质水平上表达，用构建的乳腺特异性表达载体制作转基因小鼠，利用转基因小鼠作为个体表达系统来检测目标蛋白表达量。

Recombinant adenovirus particles were produced after 293 cells transfected with recombinant Ad genomic plasmid DNA digested with PacI, then they were further amplified in 293 cells.

纯化所得腺病毒滴度约为8×1012pfu/L，当MOI=100时，腺病毒感染HepG1细胞的效率＞75％，Western Blot证实在感染重组体腺病毒的细胞中有相应基因产物的表达。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！