查询词典 plasmid

Objective to explore the action of main reagent alkaline lysis method of extracting plasmid DNA.

目的 对碱裂解法提取质粒DNA中主要试剂的作用进行研究。

To prove that the cloned DNA fragment can express tryptopanase,a new plasmid pET28C-TnaA , in which the cloned DNA fragment was located downstream of T7 promoter on pET28c was constructed and transformed into host BL21(DE3),a BL21 lysogen of bacteriophage DE3 in which the only promoter known to direct transcription of the T7 RNA polymerase gene is the lacuvS promoter ,which is inducible by IPTG.

为了证明质粒上的基因能表达出有活性的色氨酸酶，将这个DNA片段克隆到PET28c质粒的BamHI和HindⅢ位点上，使该片段受T7 RNA聚合酶的启动子控制，然后转化噬菌体DE3的溶源菌BL21(DE3)。

Objective To explore the effects of N -Has gene over expression on malignantly transformed human bronchial epithelial cell induced by anti-benzo pyrene-trans-7,8 dihydrodiol-9,10-epoxide (16HBE-T) using small hairpin RNA. Methods The recombinant plasmid pGPU6/GFP/Neo-NRas-566 including N-Has shHNA was transfected to 16HBE-T cells.

目的 用小发卡RNA(small hairpin RNA， shRNA)干扰技术沉默N-Ras基因表达，探讨N-Ras基因表达对二羟环氧苯并花转化人支气管上皮细胞(16HBE-T)恶性性状的影响。

Methods Twenty-four wells in four 6-well culture plates in which HUVECs were growing were medially divided into 2 groups with 12 holes in each group: TFPI group and empty plasmid group.

方法将长有HUVEC的4个6孔培养板中24孔平均分为2组，即TFPI转染组和空载体转染组，每组12孔。

Coli XL-1 strain, purposed cassette recombination plasmids pNB0098-K and pNB0097-K were constructed, in which the purposed gene was linked with plasmid pUC18, and the marker gene kanamycin was inserted in the purposed gene. The linear cassette DNA fragment was mixed in the medium of cultured competent cells of meningococcus BT878. The function of the purposed gene was inhibited by the homologous replace of casstte gene.

将线性的重组质粒片段置于培养基中，与感受态的脑膜炎双球菌混合培养，重组质粒中的同源性片段被脑膜炎双球菌细胞识别和摄取，整合到染色体上，目的基因被带有卡那霉素基因插入的同源性重组片段代替，导致失活，在选择性培养基上筛选出基因被敲除的突变株。

Objective To determine the invasive ability of recombinant attenuated Salmonella typhimurium X4064（pQEM1）strain containing gene fragment of Plasmodium falciparium merozoite surface protein 1 （MSP1）,the stability of its plasmid, and its re-expression.

目的 检测含恶性疟原虫裂殖子表面蛋白1基因片断的重组减毒鼠伤寒杆菌X4064(pQEM1)的入侵宿主能力，所含质粒的稳定性和再表达能力。

Since the Km〓 gene of pCS4 and pCS11 was derived from the mesophile plasmid pUB110 and coded for a thermolabile protain, it conferred Km〓 phenotype on the host cell at 48℃ only. Mutant Km〓 gene was obtained by selecting for Km〓 phenotype at elevated growth temperature. In this way improved vectors pCS5 and pCS12 were obtained, which can be used at 65℃ by Km selection.

利用提高培养温度后选择卡那霉素抗性表型的方法，从编码不耐热蛋白质的卡那霉素抗性基因出发，得到了提高其耐热性的突变的卡那霉素抗性基因，从而改造了CS4和pCS11，得到了适用温度达65℃的改进的载体质粒pCS5和pCS12。

Compared to MSM,method of plasmid elimination,it was proved that the genes encoding for degradation of phenanthrene locate on the larger one of the two plasmids.

与无机盐培养基相比，LB和牛肉膏蛋白胨培养基能促进此菌对菲的降解，尤其能促进对中间产物的进一步转化。

The study in this paper, a Pollen-specific Lysine-rich Protein cDNA, from tomato by RT-PCR was obtained. A plant expression plasmid pBIUB-TSB was constructed and lysgene was under the control of maize ubiquitin promoter which is the highest efficient monocotyledon promoter.

本实验通过RT-PCR从番茄花粉中获得高效特异表达的高赖氨酸基因TSB的cDNA序列，构建其受控于单子叶植物Ubil强启动子下的高效表达质粒pBIUB-TSB。

Part Ⅱ Objective: To study the immunoprotective and the immunotherapic effects of the new nucleic acid vaccine of the hepatitis B: pVAX1-C3d-S2S; respectively in normal and HBV transgenic mice after being inoculated intra- muscularly with this Eukaryotic Expression Plasmid, such as the in situ production of the expressed protein in muscle tissue section and specific humoral immune response in normal mice、the variation of specific antigen、antibody and HBV DNA, especialy the changes of specific antigen in liver.

第二部分新型乙肝核酸疫苗pVAX1 C3d-S〓S免疫预防效应和治疗作用的研究目的：研究含有新型分子佐剂补体C3d的HBV adw血清型的乙肝核酸疫苗重组真核表达质粒pVAX1-C3d-S〓S接种正常BALB／c小鼠和HBV转基因小鼠后，其免疫预防作用（接种局部肌肉中特异性抗原蛋白的表达和所诱生的体液免疫应答规律）以及免疫治疗作用（血清中特异性抗原的表达、特异性抗体的产生、HBV DNA的消长和肝组织中抗原表达的变化）。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！