查询词典 plasmid

Part two, The consructon of universal probe real time PCR detecting of Avian viral disease We constructed a plasmid including a specified fragment of NDV, and optimizated reactive condition of universal probe real time PCR, and detected Infectious Laryngotracheitis Viruse, Infectious Bronchitis Viruse and

第二部分：通用探针实时荧光PCR检测禽病毒病方法的建立本部分首先构建含有NDV特定片段的质粒，以提取的质粒为模板

AIM: To construct recombinant eukaryotic expression plasmid of amastin gene of Leishmania Donovani anddetectexpressionofthegeneinNIH3T3cells.

目的： 构建杜氏利什曼原虫无鞭毛体蛋白编码基因的真核表达重组质粒pcDNA31amastin，并研究其在NIH3T3细胞中的表达。

This stuty consists of five parts: In the first part, amastin gene from DNA of two Leishmania donovani isolateswas amplified respectively by PCR. Amastin gene then was cloned into prokaryoticexpression plasmid pET-32a.

本研究共分为五部分：第一部分，以两株杜氏利什曼原虫基因组DNA为模板，通过PCR扩增获得了与预期大小一致、约552bp的杜氏利什曼原虫amastin基因。

The three-plasmid packaging cell line system of recombinant lentiviral vector was successfully established.

成功建立重组慢病毒载体的三质粒包装细胞系统。

Objective To establish the three-plasmid packaging cell line of the recombinant lentiviral vector encoding rat FasL gene for eukaryotic expression to meet the requisition for deep study on the effect of FasL transgene on inducing immune tolerance and protecting allografts in organic transplantation.

目的 建立真核细胞表达的大鼠FasL基因重组慢病毒载体三质粒包装细胞系统，为进一步研究转FasL基因在同种异体器官移植中诱导免疫耐受、保护移植物的作用奠定基础。

Objective To study the construction of recombinant wild lipoprotein lipase gene plasmid and its expression in COS-1 cells.

目的 研究野生型人脂蛋白脂酶cDNA重组表达质粒的构建了及其在COS-1细胞中的表达。

Objective To construct luciferase reporter plasmid containing synthetic CArG elements and to investigate their radiation-inducible property in tumor cells.

目的构建含有人工合成CArG调控元件的荧光素酶报告基因重组质粒，研究该元件在多种肿瘤细胞中不同剂量放射线照射下的放射诱导活性。

Methods:The luciferase reporter plasmids of MDM2 P2 promoter containing different SNP309 were contructed,and cotransfected into CV-1 cells with AML1-ETO,AML1 and SP1 expression plasmid.The transactivities of luciferase were assayed by luminometer to determine the impact of AML1,AML1-ETO,and SP1 on MDM2 P2 promoter.

研究方法：构建含不同SNP309位点的MDM2 P2启动子的报告质粒，与AML1-ETO、AML1以及SP1的表达质粒单独或共转染CV-1细胞，测定荧光素酶的活性，分析AML1-ETO、AML1以及SP1对MDM2 P2启动子的影响。

Firstly, the pGL3-Control vector was reconstructed , the pGL3-Enhancer vector deletious of SV40 promoter gene was obtained . Then the IFN-β promoter gene was cloned into the pGL3-Enhancer vector and pGL-IP21, the Luciferase reporter plasmid with IFN-β promoter was OK. The availability of pGL-IP21 was verified by NDV ,the inductor of IFN-β, the Luciferase activity was assayed in cells transfected with pGL-IP21 by Luminometer.

首先将pGL3-Control载体进行了改构，除去了SV40启动子基因，获得了pGL3-Enhancer载体，将获得的IFN-β启动子基因连入载体中，构建了带有人IFN-β启动子基因的荧光素酶报告质粒IP-21，并且通过实验证明所构建的质粒在干扰素的诱导剂NDV的作用下能够表达荧光素酶活性，照度计检测荧光素酶活性增强，从而验证了所构建的重组质粒的有效性。

LyS and APRIL genes were amplified from plasmid pT7474-BLyS and human fetal brain cDNA library respectively.

淋巴细胞刺激因子是1999年发现的肿瘤坏死因子家族的一个新成员，也称为BAFF，THANK和TALL-1等[1~3]。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！