查询词典 plasmid

Altogether, these data suggest that recombinant plasmid BCSP31/pVAX1 is a good immunogen for the production of humoral and cell-mediated immune responses in mice and would be a better candidate for use in the future studies of vaccination against brucellosis.

研制的BCSP31/pVAX1重组表达质粒免疫BALB/c小鼠能产生高水平的特异性体液免疫和细胞免疫，以细胞免疫为主，并产生有效的免疫保护效果，为布氏杆菌病新型疫苗的研究奠定了基础。

An enriched PCR fragments with less complexity were obtained and mainly expressed in the denervated hippoampus After cloning into the plasmid vector, 46 clones were initially screened and subjected to autosequencing By comparison with the EMBO gene databases, 21 gene fragments of different types were obtained with 11 similar to genes of known function and 10 similar to ones of unknown function It is inferable that the altered gene expressions of hippocampus following the entorhinally denervation may underlie the specfic reinnervation and the formation of synapses by transplanted entorhinal neurons with host neuropil within the denervated target areas.

初步挑选出的46个转化后的阳性克隆，经点杂交筛选和基因序列分析，与Gene Database同源性比较后，得到21种基因片段，其中功能已知的有11种，功能未知的有10种。结果表明海马在去内嗅皮层后某些基因表达的改变，这可能是移植胚胎内嗅皮层神经细胞轴突特异支配去神经靶区和重建突触联系有关。

The recombinant baculovrius expressing the human antibody genes was isolated by co-transfection recombinant plasmid DNA with liner baculovirus DNA and purified by plaque assay, the intact recombinant human antibodies IgG-IV-2 and IgG-IV-6 was expressed in insert cells sf-9 after recombinant baculovrius infection and purified by protein G affinity chromatography.

通过空斑纯化，获得表达人抗体基因的重组杆状病毒，感染昆虫细胞进行抗体的全基因表达，表达产物经G-蛋白亲和层析纯化。

It is impossible to obtain cells, however, in which the target gene was persistently inhibited with siRNA, Therefore it is potential to use plasmid-based RNA interference technology to study gene function.

但是由于siRNA的不稳定性，采用siRNA研究基因功能不能得到抑制靶基因表达的稳定细胞克隆，因此以质粒为基础的RNA干扰技术表现出了它的应用潜力。

The recombinant uPAR plasmid intradermally injected by electroporation can promote the cutaneous re-epithelialization afterwound.

同时， uPAR在创伤下面浸润的粒细胞和创伤区域的内皮细胞中也有表达[4]，但对于创伤愈合过程中uPAR的功能研究较少。

The recombinant plasmid GST-STAT3-4 which is a truncation of STAT3 was transformed into E. coli JM109 and then induced to express a fussion protein by IPTG successfully. and we also renatured the intranuclear indusions of the fussion protein GST-STAT3-4

在实验的第二部分中我们成功的将STAT3的一个截断体STAT3-4的融合蛋白GST-STAT3-4在大肠杆菌中做了表达，并对以包涵体形式存在的STAT3-4蛋白进行了复性。

SDS-PAGE showed that the recombinant plasmid IPSE/pET32a expressed a fusion protein with an M r 35 700 after being induced by IPTG.

经IPTG诱导，SDS-PAGE分析，含重组质粒的转化子细菌能表达相对分子质量约35 700的蛋白分子，与预计Trx-IPSE融合蛋白的相对分子质量大小相符。

Methods The IPSE gene of S. man soni was synthesized by overlapping PCR, and confirmed by DNA sequencing. The recombinant plasmid IPSE-pET32a was constructed by inserting the gene of IPSE into expression vector pET32a at the downstream of thioredoxin coding sequence.

应用重叠PCR方法合成曼氏血吸虫IPSE基因，将其定向克隆至表达载体pET32a的硫氧还蛋白序列下游，构建重组表达质粒IPSE/pET32a。

Objective To explore the mechanisms of resistance plasmid in Klebsiella pneumoniae.

目的 探讨耐药性质粒在肺炎克雷伯杆菌中的耐药机制。

A Plasmid DNA vaccine pcDNA-gB expressing gB protein of infectious laryngotracheitis virus Yantai strain was constructed. The expression of viral protein from constructed DNA vaccine was confirmed by an in vitro transcription /translation system and transtection in 293-T cells.

本研究构建了编码ILTV主要抗原gB基因的重组DNA疫苗pcDNA-gB，质粒转染293-T细胞的间接免疫表明其表达的蛋白具有免疫反应性。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！