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The plasmid of pMD-T-GO and pPIC3.5K were extracted from JM109 strain and then glycolate oxidase gene fragment was cloned into the expression vector (pPIC3.5K). The recombinant plasmids were transformed into E.coli TOP 10Fˊ. The recombinant clones were identified by PCR, digested with SnaB I /Not I and then sequenced.

本文首先从大肠杆菌JM109中提取质粒pMD-T-GO和pPIC3.5K，将菠菜乙醇酸氧化酶基因c DNA片段克隆至表达载体pPIC3.5K，转化进E.coli TOP 10Fˊ，利用设计的引物进行PCR扩增、SnaB I和Not I酶切鉴定，最后进行序列分析。

Objective To construct a recombinant plasmid for expression of rabies virus glycoprotein in COS-7 cells.

目的 构建狂犬病病毒糖蛋白基因的真核细胞表达载体，并在COS-7细胞中表达。

Gnomic fragments of 4～20 kilobases were obtained by digesting genomic DNA of V.cholerae O 139 with restriction endonuclease Eco RI,then plasmid gene bank was constructed.

随机筛选重组克隆，获得一株可与O1 39霍乱弧菌抗血清发生凝集反应的重组克隆，命名为大肠杆菌DH5α(pMG32 0 )。

These three plasmid-mediated genes contribute to quinolone resistance in gram-negative pathogens worldwide.

这三种质粒介导的基因在世界范围内的革兰阴性病原体发挥着抵抗喹诺酮的作用。

Using modern biology research technique and means,plasmid extract and agarose gel electrophoresis of molecular biology level explore test were carried out for coal with not coal department the hereditary property of Thiobacillus ferrooxidans.

利用现代生物学研究方法和手段，对煤系与非煤系氧化亚铁硫杆菌的遗传特性进行了质粒抽提和琼脂糖凝胶电泳等分子生物学水平的基础性探索试验，对于质粒在硫杆菌中普遍存在的观点提出了质疑。

Human/rat homology collagen type I siRNA plasmid not only direct repress collagen type I gene expression in rat inesangial cells, but also have the same inhibition action of TGF-β1 induced overexpression of collagen type Ⅰ.

本实验构建的人鼠同源Ⅰ型胶原siRNA质粒，在大鼠系膜细胞内不仅能够直接抑制Ⅰ型胶原的基因表达，而且对TGF-β1诱导的I型胶原表达增加同样具有较好的抑制作用。

The effects of different host plant on the stability of plasmid in HA 12-1-12 and HA 7-2-2 were also assessed.

进一步考察了其中两个菌株HA 12-1-12和HA 7-2-2的质粒在四种宿主体内的稳定性，结果表明HA 12-1-12在不同宿主体内高度稳定，HA 7-2-2却发生了不同程度的变异，得到10个变异株。

Strains isolated from a single host plant showed difference in plasmid number and size.

来自同一植株根瘤的分离株其质粒型也存在多样性。

A pair of primers that contained flanking hydropathic amino acid codons were synthesized, to amply the sequence coding for 10~34 aa in the precursor sequence. The amplified fragment was inserted into pRSET-A to generate the first double repeat of the precursor gene. By utilizing a pair of isocaudamer BamH I and Bgl II sites, and another downstream Hind III site of plasmid pRSET-A, following a series of simple double digestions and ligation of the resulted products, a series of repeat (3, 4 and 6) precursor peptide fragment genes were derived.

本实验设计一对两侧含编码疏水性氨基酸密码子的引物，经过扩增前导序列10~34aa基因序列，并重新克隆入质粒pRSET-A构建串联二聚体后，再利用质粒pRSET-A的BamH I / Bgl II同尾酶克隆位点，经一系列简单的酶切和连接，快速构建这一前导肽中不含组氨酸标签序列的串联多聚体基因，并成功表达其六聚体重组蛋白。

We established the techniques of screening the plasmid expression library and cloning the immunogen genes.

建立了筛选质粒表达文库和克隆免疫原基因的技术方法。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！