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With the same method, cDNA of esterase A2 was cloned from Culex quinquefasciatus, and its expression plasmid was constructed subsequently.

使用同样的方法，从抗性蚊虫中克隆并表达了酯酶A2。

The expression plasmid pGEX-5X-1/DFF45 was constructed and transformed into E.coli BL21, and the expression of GST-DFF45 protein was induced by IPTG. After purification, the fusion protein GST-DFF45 was used to immunize Oryctolagus cuniculus to obtain the antibody serum.

构建pGEX-5X-1/DFF45原核表达质粒，转化大肠杆菌BL21，用IPTG诱导融合蛋白表达，经纯化后免疫日本大耳白兔得到多克隆抗体并用CNBr-activated Sepharose？

By using Roche kit as standard, the amplification efficiency of different reagents were compared by Roche Light Cycler instrument for establishing real-time PCR reaction system. The primers were designed based on signature sequence of chromosomal DNA fragment and caf 1 gene in plasmid pMT1 of Y.pestis. And sensitivity and specificity assay were done with serial diluted Y. pestis EV76 strain and other bacterias. Then 275 Y.

采用SYBRGreenI随机掺入法，以Roche试剂盒为标准，比较两公司的DNA聚合酶，用鼠疫菌310 0 4建立荧光PCR反应体系；针对鼠疫耶尔森氏菌特异的染色体标识序列和质粒上F1抗原基因设计引物，检测其灵敏度和特异性，以盲测试验进行验证；在此基础上鉴定2 75株鼠疫耶尔森氏菌，并测试该法检测脏器污染模拟标本的灵敏度。

Objective: To explore the apoptosis of cysticercus cellulosae induced by injection of the pcDNA3-γcCl recombinant plasmid and study the treatment effect of the DNA vaccine.

目的：观察核酸疫苗pcDNA3-γcC1免疫接种后动物宿主体内囊尾蚴的细胞凋亡现象，探讨治疗型核酸疫苗的作用机制。

PartⅡTransform the yeast, screen the excellent expression yeast strain, induce the yeast to express the fusion protein and depurate the target protein. The recombination plasmid was transformed into the yeast GS115 successfully by means of electrotransformation. The excellent yeast strain was got by G418 screening. We achived the yeast supernatant including target protein induced by methanol.

第二部分：酵母的转化、蛋白高表达菌株的筛选、重组蛋白的诱导表达及纯化实验利用电转化的方法，成功地将重组质粒pPIC9k-PTD-hA20导入毕赤酵母GS115，并通过G418浓度梯度筛选得到了蛋白高表达菌株，并对蛋白高表达菌株进行甲醇诱导表达，得到了含有目的蛋白的酵母上清液。

Since the plasmid is capable of independent replication in host cells of many dicotyledonous plants, it has been used as a cloning vector in GNETIC ENGINEERING.

在许多双子叶植物中质粒在寄主细胞中是独立的复制单元，所以可以在基因工程中用作克隆载体。

Superoxide dismutase (EC 1.15.1.1;SOD) is a metal-containing enzyme that widely exist in organisms. It catalyzes the dismutation of superoxide free radical and produces hydrogen peroxide and oxygen.It plays an important role in protecting the plasmalemma from the reactive oxygen species.A recombinant plasmid pPICZA-sodM18 was constructed and after its linearization was transferred into chromosome of Pichia pastoris GS115 by electroporation.

超氧化物歧化酶(Superoxide dismutase；EC 1.15.1.1；SOD)是广泛存在于生物体内的金属酶，是机体内唯一以超氧阴离子自由基(O_2~-)为底物的酶，它能专一地催化O_2~-的歧化反应，生成过氧化氢和氧气，能有效的抵御氧自由基和其他氧化物自由基对细胞质膜的毒性，对机体防御氧化损伤起着十分重要的作用。

And obtained recombinant plasmid PGEM-VP3 by linking prokaryotic expressionby vecto r PGEM-4T-1,and identified by PCR、double restriction enzyme analysis and sequence analysis,the result showed that it's true.

用EcoR I和XhoⅠ双酶切该片段并回收该片段，将此基因片段克隆至相同酶切回收后的PGEM-4T-1原核表达载体中，获得重组质粒PGEM-VP3，经PCR鉴定，限制性内切酶双酶切分析，证实了重组质粒的正确性。

By means of polymerase chain reactionand DNA recombinant techniques,the gene encoding mature ouse IL-4 was obtained from plasmid pCD-mIL4 and was inserted into expressionvector pBV220,which contains _RP_L double promoters,synthesized SD sequence and temperaturesensitive CIts857 gene,thus an expression lone eferred as pBV-mIL4 was constructed.

该质粒含有 P_RP_L 串联启动子，合成 SD 序列和温度敏感的CIts857基因，并且去除了完整小鼠 IL—4 cDNA 中3′侧翼非编码区，以原核系统高频使用的强终止密码子TAA 代替了原小鼠 IL-4基因的终止密码子 TAG，经 SDS-PAGE 及凝胶密度扫描分析，表达的重组小鼠 IL-4分子量约为14.2KDa，占总菌体蛋白量的25～30%。

Objective To clarify if plasmid pYC exists in other ecotype plague of China.

目的分析全国其他类型鼠疫菌是否存在pYC质粒。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！