查询词典 plasmid

To develop a new method to cline large DNA fragment by combining whole genome sequence information and the principle of plasmid rescue.

基因组测序工作的不断完成和序列信息的大量积累，为细菌基因组DNA的操作提供了方便。

Methods The plasmid expressing green fluorescent protein was used as the reporter gene, and the chitosan-pGFP NPs were prepared using a complex coacervation process.

用表达绿色荧光蛋白的质粒作报告基因，采用复凝聚法制备壳聚糖－pGFP纳米粒。

Finally we observed the condensation between the polymer and plasmid DNA by the methods of agarose electrophoresis, fluorescence spectrometer and Multi-angle laser light scattering.

最后，利用琼脂糖凝胶电泳、荧光分光光度计以及多角度激光散射手段，对合成的多聚物与质粒DNA的缩合作用进行了检测研究。

To solve this problem, we consider that use of tissue-specific regulative elements in construction of plasmid vector for molecular conjugate vector may limit the expression of foreign gene to targeting cells further.

为解决这一问题，我们考虑在构建分子偶联载体中的重组质粒表达载体时，应用组织细胞特异性调控序列，以期在转录水平进一步限制外源基因的表达于靶细胞中，从而保证受体介导基因转移的靶向性，同时可望导致外源基因的高效表达。

The results showed that plasmid DNA density was correlation to the ratio of seedset significantly but gene type and transformation time was not.

结果表明：不同基因型、转化时间对结实率影响不显著，质粒DNA浓度对结实率影响显著。

Results: Six standard serotype strains of CT were detected specifically by Plasmid Gap-LCR-ELISA with no cross-reaction to the other bacteria.

质粒Gap-LCR-ELISA对检测CT感染具有极高的灵敏度和特异度，适宜于我国进行大规模CT流行病学调查。

This causes crown gall tumors in plants, and its plasmid has been used on a range of crop plants.

这一质粒已在一系列的农作物中得到了应用。

Preventing and controlling crown gall disease applied the early biological controls strain K84, itself did not have the pathogenicity, its plasmid may synthesize one kind of bacteriocin Agrocin84, may suppress many pathogenic strains, but did not have pathogenicity to non-pathogenic strain .

防治根癌病应用较早的生防菌株是K84，本身无致病性，它的质粒可以合成一种细菌素Agrocin84，可以抑制很多有致病性的菌株，而对非致病性的菌株则无影响。

Sequences homolgous to pC4 were also found in the nuclear genomes of towel gourd and pumpkin. Therefore, we propose that mitochondrial plasmid-like DNA occurred before cucumber diverged from cucurbit and had integrated into the nuclear DNA.

在丝瓜和西葫芦的核基因组中也有pC4的同源序列，因此，推测pC4可能在葫芦科分化的早期就已存在并整合于核中。

In this research we obtained the banana ACO gene with about 1.2kb length by cuffing from the cloning vector pUCm-ACO with restriction endonucleases Xba Ⅰ and Sac Ⅰ, and then directionally linked the gene to the plasmid vector pBⅠ-121 in the same endonucleases digestion sites, finally we got the construct of plant expression vector pBI-aACO.

用限制性内切酶Xba Ⅰ和sac Ⅰ从克隆载体pUCm-ACO上切下约1.2kb的香蕉ACO基因，将其定向连接在经相同酶切的质粒载体pBⅠ-121上，构建成植物表达载体pBⅠ-aACO。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！