查询词典 plasmid

Using pUC19-SacB, we successfully constructed an unmarked deletion mutant of the VirB operon, showing that this plasmid can be efficiently used to modify the Brucella chromosome.

我们利用该质粒，成功的构建了布鲁氏菌Ⅳ型分泌系统的突变株，改建的载体能高效的应用于布鲁氏菌染色体的精确修饰。

CDNA fragment of cadherin-like protein gene in the fifth instar larva of oriental tobacco budworm was amplified using degeneracy primers and RT-PCR technique, and the target fragment was further sequenced after being cloned into pMD18-T vector plasmid.

本研究以烟夜蛾五龄幼虫中肠的总RNA为模板，根据已经克隆的其他昆虫的类钙粘蛋白基因序列设计引物，利用RT-PCR技术扩增出了烟夜蛾类钙粘蛋白基因的cDNA片段，将其连接到pMD18-T载体，转化大肠杆菌后筛选阳性克隆并进行序列测定，测序得到的1335bP的片断编码444个氨基酸残基。

Insect resistance to Bt toxin is related with the variation of cadherin-like protein gene.cDNA fragment of cadherin-like protein gene in the fifth instar larva of oriental tobacco budworm was amplified using degeneracy primers and RT-PCR technique,and the target fragment was further sequenced after being cloned into pMD18-T vector plasmid.

本研究以烟夜蛾五龄幼虫中肠的总RNA为模板，根据已经克隆的其他昆虫的类钙粘蛋白基因序列设计引物，利用RT-PCR技术扩增出了烟夜蛾类钙粘蛋白基因的cDNA片段，将其连接到pMD18-T载体，转化大肠杆菌后筛选阳性克隆并进行序列测定，测序得到的1 335 bp的片断编码444个氨基酸残基。

The recombinant plasmid containing gAd gene was transformed into E. coli BL21(DE3), and the expression of the fusion protein His-ad was induced by IPTG. Results The gAd cDNA of 412 bp was obtained from the total RNA of the fat tissue and verified by sequence analysis.

结果 从脂肪组织总RNA中扩增得到412 bp片段gAd基因，其cDNA序列与GenBank人gAd基因序列相同，并在体外表达得到相对分子质量为34 000的重组蛋白。

A conclusion can be drawn that tryptopanase expressed only by constructed plasmid during "catabolite repression".

在葡萄糖存在的条件下由于分解代谢产物阻遏，基因组上的色氨酸酶基因不表达，加入IPTG只表达质粒上的基因，如果在合适条件下测得色氨酸酶活性，则可以证明质粒上的基因能表达出有活性的色氨酸酶。

To study and know well the rudimentary knowledge and the basic techniques of modern Microbiology are demanded for Graduate students. The experiments including the principle and operation of phase contrast microscope and fluorescence microscope; Microphotography , the technique of photographic enlargement;Applications of polymerase chain reaction ; Construction, transformation and detection of plasmid in E.coli ; Infectivity of virus and its molecular detection ; The technique of protoplast fusion in filamentary fungi ; Induction and purification of β-galactosidase from E.coli and assay the enzyme activity; Detection of GC content and DNA-DNA hybridization in bacteria ; Production , extraction and detection of poly--hyduoxy butyrate from bacteria.

以实验教学为主，要求研究生学习和掌握一些现代微生物学研究所需的基本技术，内容包括：相差显微镜、荧光显微镜的使用和显微摄影技术，PCR技术的应用，细菌质粒的构建、转化及检测技术，病毒的侵染性及其分子检测技术，丝状真菌原生质体融合技术，大肠杆菌β-半乳糖苷酶的分离及纯化及其动力学测定技术，细菌G+C mol%的测定及细菌DNA-DNA杂交技术和聚羟基丁酸产生菌的发酵、产物提取及检测技术等。

Strain MCB032 which has a large plasmid can grow with chlorobenzene as the sole carbon source.

中文摘要许多微生物能够降解异源物质，而且这些降解作用的酶通常编码在质粒上。

And then, the recombinant plasmid was introduced into the component cell E.coli JM109, which transformed by CaCl2 method, and the transformant was selected on M-H agar plate supplemented with 50μg/ml of chloromycetin and 60μg/ml of ampicillin.

将新酶全编码基因克隆入表达载体pHSG398，转化于感受态细胞大肠埃希菌JM109中，用加有抗菌药物的琼脂平板进行转化株筛选。

In our study, systematically investigated the effect of chloroquine on exogenous gene expressing in mice by hydrodynamics-based naked plasmid adminstration was reported.

我们首先系统的研究了氯喹对裸质粒介导的人凝血Ⅸ因子在小鼠体内表达的影响。

The amplified full-length plasmid PCR products were transformed into competent E. coli and they were able to circularize due to the terminal homologous sequences.

转化到细菌中的全长质粒DNA PCR产物可利用其末端同源序列发生同源重组而环化。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！