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The results showed that spermatozoal SOD activity in infertile group (103±39 U/10 ̄9cells), was significantly lower than that in fertile group (144±37 U/109 cells), but no significant difference was observed in seminal plasma SOD activity between the groups.

经相关分析,精子SOD活性与精子活动率、精子正常形态及精子尾部低渗肿胀率间均呈显著性正相关;而精浆SOD活性与上述三参数、精子密度及精子SOD活性间均无显著相关性。

Therefore, fluorescein isothiocyanate conjugated lectins, namely concanavalin agglutinin, wheat germ agglutinin and soybean agglutinin, were used to localize lectin receptors on plasma membrane of male and female gametes and other relevant sexual cells, as well as embryos of various developmental stages of Nicotiana tabacum and Torenia fournieri by cooled charge coupled device and Confocal Laser Scanning Microscope.

从而证实:在本实验中用的几种酶组合对凝集素受体的影响并不大,即由酶解及解剖得到的雌性细胞的荧光在分布形式及强度上没有明显的差异;对比原位酶解与离体酶解得到的雌性细胞的荧光,证实机械操作对荧光的分布形式及强度也没有明显的影响。

The red blood cells were obtained by centrifugation of the DN plasma samples at 3000 r/min for 5 min and deproteinized with trichloroacetic acid.

和20 mmol/L pH 6.86的磷酸缓冲液,在25 kV,30 ℃和200 nm条件下,可在3 min内同时对血红细胞中GSSG和GSH定量分析。

GHK is a tripeptide found in normal human plasma. It was found to accelerate growth in cultured cells and tissues e.g. fibroblasts, macrophages.

GHK是存在于人类血浆中的一种三肽,研究表明它能促进纤维原细胞,巨噬细胞等多种细胞及组织的生长。

The cytochemical localization of POD showed that the peroxidase was found in cells ofboth cultivars after inoculation. The activity of POD in resistant cultivar was higher than that innot only the uninoculated controls but also the inoculated susceptible ones and localized morewidely, such as localized in mesophyll cell wall, intercellular spaces, membranes of chloroplastand mitochondrion and vacuolar membrane. Whereas the POD in susceptible cultivar waslocalized mainly in cell walls but little in plasma membrane with a lower range and intensitythan that in resistant cultivar.

对过氧化物酶的细胞定位研究发现,无论抗病杨树还是感病杨树,接种后均发现细胞中有过氧化物酶反应产物,通过分析发现,毛白杨过氧化物酶的活性不仅明显高于未接种的对照,也明显高于接种处理后的北京杨,并且定位更广泛,在细胞壁、细胞间隙、叶绿体膜、线粒体膜及液泡膜上均有大量定位,而北京杨酶反应产物主要分布于细胞壁上,在细胞膜、核膜局部有少量定位,定位范围及强度均小于毛白杨。

Na-K-Exchanging ATPase is a universal cell membrane protein in higher eukaryotic cells which mediates the anti-concentration gradient exchange of intracellular Na+ for extracellular K+. It plays essential metabolic roles such as maintaining sodium and potassium ion gradients across the plasma membrane. The targeted sequences were amplified by PCR to determine the genome structure of the hithertofore unsequenced portion of the alpha 1 subunit of the human Na-KExchanging ATPase gene which encodes 80-130 aa of its extracellular domain using two different templates, human genomic DNA and a human muscle cDNA library. The PCR products were analyzed by restriction endonuclease digestion and then cloned into a plasmid vector for chemiluminescence sequencing and further analysis.

Na-K-Exchanging ATPase是一种普遍存在于高等生物体内的细胞膜蛋白,主要参与介导K+和Na+在细胞内外之间的逆浓度梯度的转运,并维持一定细胞内外的离子梯度,我们采用聚合酶链式反应方法分别以人基因组DNA及cDNA文库为模板对人Na-K-Exchanging ATPaseα1亚单位基因胞外区约80-130位氨基酸编码序列进行扩增,限制性酶切分析扩增产物,并进行荧光测序,对测序结果进行同源性分析及剪接位点的搜索并对得到的核苷酸序列进行进一步的分析,发现人基因组DNA和cDNA经过扩增后分别得到833bp和195bp两种不同大小的片段Fg,Fc。

Itamin D from the skin and diet is metabolized in the lier to 25-hydroxyitamin D (Figure 1), which is used to determine a patient's itamin D status1,2,3,4; 25-hydroxyitamin D is metabolized in the kidneys by the enzyme 25-hydroxyitamin D-1-hydroxylase (CYP27B1) to its actie form, 1,25-dihydroxyitamin D.1,2,3,4 The renal production of 1,25-dihydroxyitamin D is tightly regulated by plasma parathyroid hormone leels and serum calcium and phosphorus leels.1,2,3,4 Fibroblast growth factor 23, secreted from the bone, causes the sodium–phosphate cotransporter to be internalized by the cells of the kidney and small intestine and also suppresses 1,25-dihydroxyitamin D synthesis.5 The efficiency of the absorption of renal calcium and of intestinal calcium and phosphorus is increased in the presence of 1,25-dihydroxyitamin D (Figure 1).2,3,6 It also induces the expression of the enzyme 25-hydroxyitamin D-24-hydroxylase (CYP24), which catabolizes both 25-hydroxyitamin D and 1,25-dihydroxyitamin D into biologically inactie, water-soluble calcitroic acid.2,3,4

从皮肤和食物来的维生素D在肝中代谢为25-羟基维生素D(图1),被用来决定病人体内维生素D情况的1,2,3,4;25-羟基维生素D在肾中被25-羟基维生素D1羟化酶(CYP27B1)转变为有活性的1,25-二羟基维生素D 。1,2,3,4由肾产生1,25-二羟基维生素D是被血浆甲状旁腺激素和血清钙,磷水平紧密调节。1,2,3,4由骨分泌的成纤维细胞生长因子23使钠磷协同转运蛋白被肾和小肠细胞内化及抑制1,25-二羟维生素D合成。5 在1,25-二羟基维生素D作用下肾和小肠吸收钙及磷的效率增高(图1)。2,3,6 它也包括25-羟四- 24 -羟化酶的表达(CYP24),且将1,25二羟基维生素D和25羟基维生素D异化成无生物活性,水溶性的维生素D3-23羧酸。2,3,4

OBJECTIVE: To evaluate the effects of platelet-rich plasma and the fascia with blood vessels on the vascularization of tissue-engineered bone composited by marrow stromal stem cells and decalcified bone matrix.

目的:拟从组织影像学角度评价富血小板血浆及带血管筋膜对骨髓基质干细胞/脱钙骨基质复合的组织工程骨血管化作用效果。

OBJECTIVE: To investigate reversible metabolic pharmacokinetics of prednisone and prednisolone in plasma and blood nucleated cells in 10 healthy male volunteers.

目的:研究强的松、强的松龙在10名男性健康志愿者血浆及血中有核细胞内的可逆代谢药代动力学。

The plasma and nucleated cells were separated from blood for HPLC analysis of prednisone, prednisolone and cortisol.

从血样中分离出血浆和有核细胞后用HPLC法测定强的松、强的松龙和可的松的浓度。

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