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Through the research we have established in planta transformation system, mutangenesis and screening system for salt sensitive mutants, and constructed a cDNA library under salt stress and cloned a serious of salt-tolerance related genes which had been confirmed the function by overexpression in Arabidopsis or antisense expression in Thellungiella. We also compared different mapping strategy through the analysis of the genetic polymorphism between ecotypes, and establishing mapping population for physical mapping.

建立了小盐芥的转化体系与盐敏感突变体诱变与筛选体系,构建了盐胁迫下的cDNA文库并克隆了一批耐盐相关基因,并通过模式植物拟南芥转化与小盐芥的基因反义表达证实其功能,通过不同生态型小盐芥的遗传多样性分析探讨了基因组作图的策略,建立了作图群体并开始进行物理图谱构建。

Demming B,Bjorkman O (1987). Comparison of the effect of excessive light on chlorophyll fluorescence (77K) and photo yield of O2 evolution in leaves of higher plants. Planta,171: 171~184Guo LW,Shen YG (1996). Protective mechanisms against photodamage in photosynthetic apparatus of higher plants. Plant Physiol Commun,32(1):1~8 in Chinese

孟庆伟等(1996)认为光呼吸可减轻光抑制,尤其在发生光合午休时更明显,而对光合作用却有反作用,Zelitch (1992)的研究表明光呼吸随温度的升高而升高,因此高蛋白小麦中午较强的光呼吸作用可能是其Fv/Fm下降较少,而净光合速率下降幅度较大的一个重要原因。

Strains of Pseudomonas syringae pv. syringae secrete syringolin A, a product of a mixed non-ribosomal peptide/polyketide synthetase, in planta.

菜豆细菌斑点病菌可在植物中分泌syringolin A,一个非核糖体肽和多聚乙酰合成酶的混合产物。

WANG L J%JIANG W B%HUANG B J Promotion of 5-aminolevulinic acid on photosynthesis of melon seedlings under low light and chilling stress conditions 10.1111/j.0031-9317.2004.00319.x Physiol Planta , 2004,null

汪良驹%姜卫兵%黄保健 5-氨基乙酰丙酸对弱光下甜瓜幼苗光合作用和抗冷性的促进效应园艺学报, 2004,3

The main results are as following:(1) Arabidopsis in planta transformation were made by Agrobacterium tumefaciens carrying the activation tagging vector pSKI015 with herbicide-resistant Bar gene as a selective marker. About 12 000 independent transformant lines were acquired by the above method, from which two mutants related to plant defense response and development, asr1 (auxin and .salicylic acid responsive 1.) and dai1 (brought tolerant and obscisic acid insensitive 1) were isolated.

本文以拟南芥为实验材料,以激发标签为技术平台,构建了拟南芥的功能获得突变体库,从中筛选具有抗病、抗逆表型的突变体,克隆基因并进行基因的功能研究,取得的主要结果如下:(1)利用含激发标签质粒pSK1015的农杆菌直接转化拟南芥植株,以抗除草剂的Bar基因为筛选标记,获得了约有12000个独立转化株系的突变体库,并从中筛选到2个与抗病、抗逆和发育相关的突变体asr1(auxin and salicylic acid responsive 1)和dai1(drought tolerant and abscisic acid insensitive 1)。

This conclusion is based on three groups of experiments with detached root tips of wheat seedlings: By following the time course changes of NO synthase , superoxide synthase and ABA accumulation under dehydration stress, we found that ABA accumulation was preceeded by the changes of NOS and superoxide synthase. ABA accumulation induced by dehydtrion stress and osmotic stress can be blocked by NO scanvegers and NOS inhibitors, while only dehydration stress-induced ABA accumulation can be blocked by ROS scanvegers. Exogenous applied NO or ROS induced ABA accumulation in root tips of wheat seedlings, and synergistic effects were found between NO and NO. The in planta expereiments also proved that ROS and NO are involved in water stress-induced ABA accumulation.Ⅱ.

证据主要来自以离体根尖为材料的实验:(1)通过检测干旱胁迫下小麦根尖NOS活性、O〓合成酶活性和ABA积累的时间变化进程,发现ABA积累滞后于ROS和NO的变化;(2)抑制剂实验表明,NO清除剂和NOS抑制剂可以抑制自然干旱胁迫及渗透胁迫诱导的小麦根尖ABA积累,ROS清除剂抑制自然干旱胁迫诱导的ABA积累而不抑制渗透胁迫诱导的小麦根尖ABA积累;(3)在非胁迫条件下施加外源的ROS或NO可以诱导小麦根尖积累ABA,ROS和NO之间具有协同作用。

The main work of this dissertation is constructing Arabidopsis gain of function mutants pool, screening mutants related to plant defense response, cloning the genes and studying their functions. The main results are as following:(1) Arabidopsis in planta transformation were made by Agrobacterium tumefaciens carrying the activation tagging vector pSKI015 with herbicide-resistant Bar gene as a selective marker. About 12000 independent transformant lines were acquired by the above method from which two mutants related to plant defense response and development, asr1 (auxin and salicylic acid responsive 1) and dai1 (drought tolerant and abscisic acid insensitive 1) were isolated.

本文以拟南芥为实验材料,以激发标签为技术平台,构建了拟南芥的功能获得突变体库,从中筛选具有抗病、抗逆表型的突变体,克隆基因并进行基因的功能研究,取得的主要结果如下:(1)利用含激发标签质粒pSKI015的农杆菌直接转化拟南芥植株,以抗除草剂的Bar基因为筛选标记,获得了约有12000个独立转化株系的突变体库,并从中筛选到2个与抗病、抗逆和发育相关的突变体asrl(auxin and salicylic acid responsive l)和dail(dought tolerant and abscisic acidinsensitive l)。

Compared with plant density of 150×10^4 planta/hm^2, photosynthesis attenuation rate of traditional plant density of 450×10^4 plants/hm^2 increased by 25.2~43.5 percent point, photosynthesis yield and relative electron transport rate of them decreased 2.95%~20.68% and 10.66%~18.32%, respectively, and the proportion of the reception of photosynthesis active radiation increased upper the layer of flag leaf, and decreased the proportion received lower the layer of flag leaf.

播量为450万株/hm^2处理比150万株/hm^2处理旗叶的光合衰减率提高25.2~43.5个百分点,光合量子产量和相对电子传递速率分别降低2.95%~20.68%和10.66%~18.32%,群体上层的光截获量增加,中下部受光条件较差。

This article analyzes the significance and the method of the evaluation of the forest carbon-sink,taking the artificial planta- tion of the Three-north shelterbelt forest system program as an example,uses the variable BEF (Biomass Expan- sion Factor) method to assess the value at the Three-north shelterbelt forest carbo.

对森林碳汇价值评价意义和方法进行了分析,并以三北防护林体系工程人工林为例,采用换算因子连续函数法对三北防护林的碳汇价值量进行了评价,提出了持续开展三北工程、提高人工林固碳能力的对策建议。

Demming-Adams B%AdamsⅢ W W Xanthophyll cycle and light stress in nature:uniform response to excess direct sunlight among higher plant species Planta , 1996,null

潘秋红%朱靖杰%蔡世英%胡耀华不同光习性植物光合诱导现象的研究热带作物学报, 1996,02

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