查询词典 pilot a ship into harbour

The carbons in explosives are decomposed into gas-like carbon atoms in the detonation reaction zone, then unoxidized carbon atoms coagulate into carbon liquid droplets and finally consolidate into solid diamonds The new mechanism differs from one conclusion that carbons of explosives are at first transformed into solid graphites, then into diamonds, and also differs from the conclusion that gaseous carbons coagulate into liquid droplets in the reaction zone, then crystalize into diamonds in Taylor rarefaction wave zone.

修正了有关炸药中的碳在反应区内先转变为石墨，然后石墨再发生相变生成金刚石的论点以及认为在反应区内气态碳凝聚为碳液滴，液滴在Taylor稀疏波区凝固成金刚石的论点。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Hey yo jim man why don't ya, you don't you kick some of that you know you know how you do it man it's a trip people don't even believe were together right now but tell your story you know the one i like say it for me riders on the storm riders on the storm into this house we're born (into this house we're born) into this world we're thrown (into this world we're thrown) like a dog without a bone an actor out on loan riders on the storm there's a killer on the road his brain is squirmin' like a toad take a long holiday let your children play if ya give this man a ride sweet family will die killer on the road, yeah goin' off of this off of that with the lizard king bumpin' in the back how bout that driftin', liftin', swiftin', coastin', testaroastin' but the wheels won't stop 200 on the highway fresh up off the block he's a rider, na he's a killer dresses in all black but his hat says stealla pedal to the metal i gotta go hard drive by and say hello hey fredwreck you my mello now let me here what i sound like acapella wow ride dip swish now bring it back just like this like a dog with out his bone unlike a g with out his chrome it's hard to imagine the homey dog in a jag and he's checkin' for the checkered flag comin' in first never in last cause my car to fast i neva eva run out of gas cause i just to clean i do it upper class so fasten your seat belts its so hot it will even make heat melt so get a bowl and roll and ride slip through the slip and slide like a dog without a bone an actor out on loan riders on the storm there's a killer on the road his brain is squirmin' like a toad take a long holiday let your children play if ya give this man a ride sweet family will die killer on the road, yeah need for speed i'm trying to take the lead hold on little homey before you run into the trees i've seen thinks that i would have never saw before hey yo jim let'em in, let'em in open up my back tire smokin' the whole street and now the police wanna flash there lights and chase the dogg all night but i won't pull over nor give up cause i just don't give a fuck yeah from the side boy where we was born and raised straight up to ride boy continuously, we get to an expeditiously keep the light on east side on snoop dogg and the doors and yeah we bout to ride on riders on the storm riders on the storm into this house we're born into this world we're thrown were thrown

嘿摇摇弯轨机人为什么不唉，你不你踢一些那你知道你知道如何你这样做人这是一次旅行人们甚至不相信是在一起此时但告诉你的故事你知道那个我像为我说它骑士在暴风雨上骑士在暴风雨上入这房子我们是出生入这世界我们是扔像个狗没有一根骨头一个演员那儿有一个杀手在路上他的大脑是squirmin'像个癞蛤蟆采取一个长的假期让你的孩子们玩如果唉给这人一个乘甜家将死凶手在路上，是呀去'离开对于这离开关于那和蜥蜴国王 bumpin'在后面如何拳击比赛那 driftin'，liftin'，swiftin'，coastin'，testaroastin'但是这个车轮不会停止两百在公路上新鲜向上远离块他的一个骑士，娜他的一个杀手衣服在纯黑但他的帽子说stealla 踩踏板金属我有去难驶过和问好嘿fredwreck 你我的梅洛现在让我这里什么我听起来像acapella 哇骑现在倾斜豪华带来它回来只是这样像个狗与出来像个狗没有一根骨头一演员借出的骑士在暴风雨上那儿有一个杀手在路上他的大脑是squirmin'像个癞蛤蟆采取一个长的假期让你的孩子们玩如果唉给这人一个乘甜家将死凶手在路上，是呀需要速度我是试图着先鞭继续小像家一样在你未碰撞树木我已经看到想那我从来不锯在以前嘿摇摇弯轨机让'em 在，让'em 在开发我的后面疲劳smokin'一街一巷那么警察要闪光那儿灯光和追逐道格整夜但我不会拦截也不放弃造成我只是不给一个性交是呀自边男孩我们在那里出生和提升直直至骑男孩骑士在暴风雨上骑士在暴风雨上入这房子我们是出生入这世界我们是扔我的翻译不太标准别见怪

About 6 per cent of patients over 60 years of age harbour an adrenal adenoma.

年长于60岁的人中有6%患有肾上腺瘤。

The design for Campus Palmas Altas – by Rogers Stirk Harbour + Partners and Vidal y Asociados arquitectos – aims to creates a new model for the business park, one that is not only more compact and urban in character than conventional business parks, but also particularly suited to the extreme summertime conditions prevalent in the south of Spain.

这座为帕尔玛校园设计的科技园，是由RSH合伙人事务所和Vidal y Asociados建筑事务所设计的，旨在构建一座新的商务园区模型区，不仅比传统的商务公园更加紧凑和城市化，而且特别的适合西班牙南部夏季的极端气候条件。

The main islands of the archipelago are New Providence, with Nassau as the capital, Grand Bahama, Abaco, Andros, Bimini, Cat island, Eleuthera, Harbour Island, Exuma and San Salvador.

巴哈马群岛主要岛屿包括新普罗维登斯岛，以拿骚为首都，大巴哈马，阿巴科，安德罗斯，比米尼群岛，卡特岛，伊柳塞拉，哈勃岛，埃克苏马和圣萨尔瓦多。

Danish architects MAPT have won first prize in a competition to design a cafe in the Anker Brygge harbour in Oslo, Norway.

丹麦建筑师MAPT赢得首奖的竞争中设计一个咖啡馆的安克Brygge港湾在挪威首都奥斯陆。

I alos went abroad to Sahara Desert in South Africa , the Arctic Circle in North Pole , Sydney Harbour Bridge and Sydney Opera House in Australia, Eiffel Tower and Arc de Triomphe in France, Channel Tunnel under West Pacific Ocean , Big Ben and Tower Bridge in London, New York in the United States of America in North America , and Berlin in East Germany , Bonn in West Germany .

我也出国去了南非的撒哈拉沙漠，北极的北极圈，澳大利亚的悉尼港大桥和悉尼歌剧院，大西洋底下的英法海底隧道，伦敦的大本钟和塔桥，北美洲美国的纽约，东德的柏林与西德的波恩。

Spot the endangered orange-bellied parrot around Bathurst Harbour between October and March.

十月到三月间，在巴瑟斯特海港观看濒危的橙腹鹦鹉。

Fishing boats jostle with designer yachts in the harbour of Honfleur; contemporary restaurants and chic boutiques sit alongside half-timbered houses and Gothic churches in Rouen; and the reconstructed centre of Caen is a short drive away from the cobblestones of Bayeux.

Honfleur 港里挤满了渔船和名牌快艇；在 Rouen ，现代的大饭店和别致的时装小店与半木制房屋和哥特式教堂交错呈现；重建的 Caen 中心与巴约的鹅卵石仅仅几步之遥。

Nowadays, most of research are to build a transmutative Petri Nets through adding controlling place sets, controlling arc sets and controlling policy to the basic Petri Nets, while the Controlled Petri Nets could be used to argue many controlling theory problems conveniently and to induce many logically and physically supervisory and solve the Event Feedback Controlling Problems and State Feedback Controlling Problem in DEDS supervisory theory.

目前大多数的研究表现为在变形后的受控Petri网基础上，利用各种方法求得各种逻辑型、结构型控制器，解决DEDS监控理论中的事件反馈控制问题与状态反馈控制问题。

On one hand, there are discussions with the works council and union about extension of short time working up to the end of September.

一方面，有讨论，工程理事会和联盟关于延长工作时间短至9月底。