查询词典 pigs

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

Therefore Herbert and Gideon Spilett, with Top in front, traversed moreoften than their companions the road to the corral, and with the capitalguns which they carried, capybaras, agouties, kangaroos, and wild pigs forlarge game, ducks, grouse, jacamars, and snipe for small game, were neverwanting in the house.

因为鸟类大大地增加，这些建筑物非加以扩大不可了。厩房里现在有五头野驴，除了一头小驴以外，其余的四头都训练得很好，既肯拉车子，又肯让人骑。小队现在有一张犁，他们常常用两头野驴拉犁，象约克州和肯特基的真正的耕牛一样。

Tip : Did you know guinea pigs eat special droppings called cecal feces?

附：你知道猪猪吃掉的自己的排泄物被称为盲肠排泄物嘛？

But what depressed her most is that her reward included 3 pigs, 23 turkeys,44 chooks and 5,000 coconut,along with a large deal of bananas, lemons and oranges.

然而令她懊恼的是，这些报酬包括3头猪，23只火鸡，44只鸡和5000个椰子，还有相当数目的香蕉，柠檬和橘子，理论上等于票房收入的1/3。

I've collected some Chinese trumpet creepers to feed the pigs.

我从山上采了些苕子回家喂猪。

This story uses critters instead of pigs, you have a great memory!

这个故事用小动物代替了小猪，你有很好的记忆力！

What is A, the pigs are B and the curring process begins.

What is A放到这里就不知道在这句子里的逻辑上有什么意思了。

No cysticercus cellulosae was found in various organs of pigs 10 days after infection; but asmall amount of immature form could be detected in liver 15-30 days after infection, the number of cysticerci parasitized in liver gradually increased.

感染40~70d时在肝脏寄生的囊尾蚴数量增多，同时开始在前肢和后肢的肌肉、胸肌、颈肌、脑组织、肾脏、肺脏内逐步见到未成熟的囊尾蚴。

Cysticercus ellulosae could live in liver not only in muscle as we thought before. During the fast process of sending pigs to go into the market, it should be aware that even though cysticerci are not found in liver, they may be still in suitable locations of muscles, and more attention should be paid to pig quarantine.

猪囊尾蚴除在四肢肌肉等主要寄生部位生长外，还可以在猪的肝脏内寄生，但是随感染时间增加会逐渐死亡和钙化，表明在当前生猪快速出栏养殖过程中，猪肝脏无囊尾蚴寄生时，其余主要寄生部位如四肢和驱干肌肉内仍有囊尾蚴寄生，提示在生猪检验检疫工作中应予以重视。

Exercise training improves femoral artery blood flow responses to endothelium-dependent dilators in hypercholesterolemic pigs.

在体的内皮依赖性舒血管反应通过测量动脉内注射腺苷二磷酸和缓激肽后股动脉血流的变化来评定。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失