查询词典 pi

The periportal red hyaline globules seen here with periodic acid-Schiff stain are characteristic for alpha-1-antitrypsin deficiency (a person with homozygous pi-ZZ genotype) The globules are intrahepatic collections of alpha-1-antitrypsin that is not being excreted from the hepatocytes .

1-抗胰蛋白酶缺乏症可见典型的病理变化是经 PAS 染色，可见肝细胞内有圆形红染半透明的玻璃样小滴。小滴是肝内α1-抗胰蛋白酶聚集不能被肝细胞排泄而形成的。

Based on petrographical observations in red rutile ore, PI is possibly the transitional phase and complete reactants are Ath + Phi.

红色金红石矿中金红石成分均匀，未见有钛铁矿的出溶现象，黑色金红石矿中金红石存在明显的厚度可大于2μm钛铁矿出溶叶片。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Reverse-transcription PCR assays were used to detect the mRNA expression level of related regulatory genes such as p15, p16, p21, p27, p57, surviving, cyclin B, cdc2 and chk1, and Western blot assay to detect the level of protein expression and phosphated change such as survivin, cdc2 and chk1. Results:(1) K562 cell arrested on G2/M phase were obviously increased after co-cultured with 2 to 10μmol/L Arsenic trioxide for 24 hours. The ratios of control group, 2μmol/L, 5μmol/L and 10μmol/L groups were 22.6±3.4%, 27.2±2.3%, 43.8±4.5% and 36.7±4.1%, respectively.

体外培养K562细胞，以不同浓度AS_2O_3作用不同时间后采用PI染色、流式细胞仪检测药物作用后细胞的周期分布改变；逆转录酶多聚酶链扩增方法检测细胞周期相关调节基因(p15、p16、p21、p27、p57、survivin、cyclinB、cdc2、chk1)的mRNA表达变化；Western blot方法检测细胞周期相关调节蛋白表达及磷酸化改变(survivin、cdc2、cdc2-p、chk1)。

Amino-monomethoxypoly(ethy1ene glycol)(mPEG-NH2) was synthesized based on the principle of Gabriel synthesis.Monomethoxypoly(ethy1ene glycol) tosylate was prepared at first,and then reaction with potassium salt of phthalimide as nucleophile to produce the monomethoxypoly(ethy1ene glycol)phthalimide derivative.

摘 要：首先合成了单甲氧基聚乙二醇对甲苯磺酸酯，然后根据盖布瑞尔合成法原理，以邻苯二甲酰亚胺钾盐为亲核试剂与mPEG-OTs反应，生成单甲氧基聚乙二醇的邻苯二甲酰亚胺衍生物，mPEG-PI与水合肼反应肼解生成伯胺，合成了一端为氨基的单甲氧基聚乙二醇(mPEG-NH2)。

In this paper ,move ment pri nci ple of rocket i n direction pi pe was got by dy— namic si mulation ,and effect characteristic consideri ng contact stiffness ,detent force and nozzle thrust misalignment a— bout pitchi ng angle velocity was analysis .All the study set foundationfor further study of high—lowpressure launch sys— te m.

通 过发射仿真分析，获得了火箭弹在高低压发射系统中的内弹道变化规律，分析了火箭弹与定向器的接触刚度、耳轴扭转刚度、尾喷管推力偏心等因素对俯仰角速度的影响特性，所得结论为火箭武器高低压发射系统的进一步相关研究奠定了基础。

Pneumatosis intestinalis and portomesenteric venous gas are alarming radiological findings that signify bowel ischemia. The management of PI and PMVG remain a challenging task because clinicians must balance the potential morbidity associated with unnecessary surgery with inevitable mortality if the necrotic bowel is not resected.

肠气囊肿病和门脉肠系静脉积气在影像诊断学上是缺血性肠道疾病非常重要的表现，当病人的影像出现肠气囊肿病或是门脉肠系静脉积气时，临床医师要如何作最适当的处置，依然是一项富挑战性任务，因为如果坏死的肠组织没有被切除，无可避免的会造成病人死亡，但是多余的手术亦会有潜在的并发症。

To fulfill the requirements of reliability, extensibility and adaptability, this dissertation proposes a Holon-based control architecture for flexible manufacturing shop floor using a bottom-up design approach. The inner structure of component Holon, the relationships of data and functions among Holons, the model of message transfer and the basic specification of communication language are described in detail. Then, from the aspect of software architecture, a formal description and analysis of the proposed architecture is conducted by First-order Polyadic pi-Calculus.

针对可靠性、可扩展性和适应性等要求，提出了基于Holon的柔性制造车间控制体系结构，描述了组件Holon的结构模型、数据和功能关系、信息传递模型和通信语言基本规范，并从软件体系结构的观点出发，运用π演算对其进行了形式化描述和分析，为基于Holon的体系结构设计和分析奠定基础；3。

Queste le specifiche del nuovo MacBook Air di Apple , il portatile più piccolo al mondo .

这些规范的新的苹果MacBook空气，最小的便携式的世界。

DNA content within 25 cholesteatoma tissues and 10 normal postauricular skin was measured with flow cytometry.

利用机械捣碎法将新鲜的胆脂瘤和外耳道上皮制成单细胞悬液；采用PI法，对25例胆脂瘤上皮和10例正常外耳道细胞上皮的DNA进行流式细胞术分析。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。