查询词典 pi-dog

We have different PI es, such as apple, cherry, and strawberry PI es.

我们有不同的派，例如苹果、樱桃和草莓派。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

The electrets properties of homemade ZPKI PI films were studied by comparing with Kapton PI films supplied by Dupont.

通过和杜邦公司产KaptonH商品膜驻极体性能比较，系统研究了由国产ZPKI型聚酰亚胺前体溶液制备薄膜的驻极体性能。

In a word, we extracted first cotyledon of buckwheat total RNA, by method of RT-PCR and 3RACE, get the C terminal cDNA sequence of PI from Ukraine buckwheat, and do some bioinformatics analyse on this cDNA sequence of PI from buckwheat, such as gene characteristics, expression mode. All of this will be help for the forward research on functional protein and high performance of procaryon expression or eukaryon expression.

综上所述，我们利用RT-PCR和3′RACE技术首次从吉乌一号甜荞第一对子叶中克隆获得荞麦蛋白酶抑制剂基因并对其基因特性、表达模式进行生物信息学分析，同时构建了该基因序列羧基端原核表达载体，为今后进一步研究荞麦功能性蛋白的分子机制及高效原核、真核表达载体的构建奠定了基础。

Clean of deep of Zheng of king of famous fairy tales is taken up the post of by 2000 " Pi Pi happy overturn the heavens " literature is advisory, it may be said is open up integrated and effective resource, a kind of when stimulative industry grows quickly new approach.

著名童话大王郑渊洁2000年底出任《霹霹乐翻天》文学顾问，可谓是开辟了整合有效资源，促进产业快速发展的一种新途径。

Ringrazio molto i ragazi del sito, ma non posso più perdere tempo,, pèer carattere non riesco a lasciar cadere le provocazioni continue, percui non mi resta altro che lasciare più spazio per scrivere al genio creativo di chi non sa far altro che essere contro senza mai commentare propositivamente ne con rispetto le notizie pregevoli da voi riportate.

非常感谢你ragazi网站，但我不能失去更多的时间，同侪不能继续下降的挑衅，所以我只能留出更多空间来写入的创造性天才的人谁不能不将不propositivamente他们从来没有发表评论的新闻比你的宝贵的报告。

The daily life of pi is a frequently used data, but under different circumstances, we need the accuracy is different, so one must be prepared through various different precision pi accuracy to meet the corresponding requirements.

详细说明：圆周率是日常生活经常用到的一个数据，然而在不同的情况下，我们需要的精确度是不同的，所以必须编写一个通过不同精度要求显示不同精确度的圆周率，以符合相应的要求。

Although they do not like light and graceful in posture petals; though, they are not delicate butterflies fluttering in the body may be they do not want these, they want, is strong, is strong, is the burning issue of life and the "Pi Pi strip stripping "of Bao Xiang, a paean to life!

虽然，它们没有花瓣般轻盈婀娜的身姿；虽然，它们没有蝴蝶翩翩飞舞娇柔的体态可它们并不想要这些，它们想要的，是壮，是烈，是生命燃烧时发出的&噼噼剥剥&的爆响，是生命的赞歌！

The results showed that CoPI films were amorphous state and their glass-transition temperatures T(subscript g and decomposition temperatures T(subscript d respectively occurred above 322℃ and 544℃. At the same time, the tensile strengths of CoPI films reached beyond 100MPa and breaking elongations were improved from 12% to 54%, but the average of tensile modulus was reduced by 29%. However, polyimide blends had about the same structures and properties with PMDA/ODA-polyimide.

结果表明，CoPI薄膜为非晶态结构，玻璃化转变温度和起始分解温度分别在322℃和544℃以上，断裂伸长率由12%平均提高到54%，拉伸强度均超过100MPa，但弹性模量平均降低了29%；共混PI的聚集态结构和性能与PMDA/ODA型PI相近。

12The following appeared in a memo from a vice president of Alta Manufacturing.

1作者认为我公司比 PI 多30%工伤是由于 PI 比我们的换班周期短1小时。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。