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Method］ Solid and liquid medium of the nitrogen-free BAP, S, JA, ISP4, CB-CcI3, DPM, FTW were used to isolate and purify root nodules of Elaeagnus and Hippophae collected from Tongliao and Chifeng in Inner Mongolia. According to determination results of 16S rDNA sequence, a phylogenetic analysis was carried out.

方法］以通常用于分离弗兰克氏菌属固氮放线菌的无氮BAP、S、JA、ISP4、CB-CcI3、DPM和FTW等为固体和液体培养基，对采集自内蒙古通辽和赤峰的沙棘和沙枣植物根瘤进行放线菌的分离纯化，并依据16S rDNA序列测定结果对分离物进行系统发育分析。

Results Fifty-one clones screened from 160 clones on the basis of Hae III digestion patterns were sequenced, and their sequences were deposited in the GenBank. Clone sequences (52.9%) belonged to Acidimicrobidae and 5 suborders of Actinobacteridae. The other clone sequences (47.1%), which formed one large distinct clade in phylogenetic tree among phylum Actinobacteria, may represent one new suborder or new class.

所获得的51个克隆序列属于39个OTUs，其中52.9%的克隆序列分布于放线菌门放线菌亚的5个亚目和酸微菌亚纲中，并且在这两个亚纲中有大量克隆序列属于放线菌的新类群，另外47.1%的克隆序列以极高的自展值在放线菌门内支持形成一个独立的大分支，极有可能代表一个新亚目或更高级分类单元的类群。

Phylogenetic tree reconstruction showed PmSERK1 and other key SERK genes involved in evoking somatic embryogenesis such as MtSERK1, CuSERK1, AtSERK1, and DcSERK, were classified to one cluster.

系统进化树重建表明，PmSERK1与已知参与体胚发生的关键SERK基因聚为一类，如MtSERK1、CuSERK1、AtSERK1和DcSERK。

Currently, it is a highly common sense that pig is the suitable donor of organs, but the great phylogenetic distance between pig and man may induce three kinds of rejection, Hyperacute rejection , Delayed Xenograft Rejection and T cell-mediated Rejection .

现在公认的理想器官供体是猪，但猪的器官移植到人体内会发生三个阶段的排斥反应：超急性排斥反应(Hyperacute Reiection，HAR)、延缓性排斥反应(Delayed Xenograft Rejection，DXR)以及T细胞介导的排斥反应(Tcell-mediated Rejection，TMR)。

In order to elucidate the relationship between the structural features of leginsulin gene in legume plants and their phylogenetic significance,we have cloned the cDNA sequence of leginsulin gene from radicles of broad bean via RT-PCR techniques according to the leginsulin gene sequence we previously obtained from soybean.The cloned cDNA encoded for a precursor protein consisting of the signal peptide,mature leginsulin and an additional 45 amino acids of another polypeptide.

为探明豆科植物中豆类胰岛素基因的结构特征与进化关系，在已获得大豆豆类胰岛素基因的基础上，以蚕豆种子胚根mRNA为材料，采用RT-PCR技术，克隆了蚕豆豆类胰岛素基因的cDNA序列，编码的前体多肽包括信号肽、成熟型豆类胰岛素及另一多肽的45个氨基酸残基。

Provirus DNA was extracted, HIV-1 gag genes were amplified by nested-PCR and sequenced directly with pairs of specifically designed primers, sequences were then analyzed by GCG software system to determine genotypes, and phylogenetic trees were established.

采集17名感染HIV的农村外来妇女及这些外来妇女的4例阳性配偶的外周血，提取前病毒DNA，应用套式PCR进行艾滋病病毒gag基因检测，使用GCG软件包对扩增序列进行分析，并对这些HIV感染者进行相关流行病学调查。

On the basis of phylogenetic analyses, three isolates, strain 117,43401 and 12202 were subject to a polyphasic taxonomic study.

实验结果显示，酸性土壤中的嗜酸稀有放线菌具有较丰富的种属多样性，其中诺卡氏菌属是一优势菌群。

A study on polyphasic taxonomy and phylogenetic diversity of cold-adapted bacteria;;Characterization and purification of cold-adapted protease

中文题名低温菌的多相分类学及系统发育学研究；低温蛋白酶的分离纯化及性质

Based on the polyphasic studies, including its morphology, physiological and biochemical characteristics, chemotaxonomy and phylogenetic analysis by using 16S rDNA sequence comparison, YIM 80186 was primarily identified as a new potential species of the genus Nocardiopsis genus.

通过对其进行的包括形态、生理生化特性、细胞化学以及基于16S RNA基因序列的系统发育分析等方面的多相分类研究，该菌株具有拟诺卡氏属的典型特征，初步鉴定其为拟诺卡氏菌属的1个潜在新种。

Based on the principle of polyphasic taxonomy, investigation of the phylogenetic relationships of Lachnum and the related genera by using physiological and biochemical characteristics, analysis of esterase isoenzyme, GC-MS analysis of volatile components from fermentation broth and sequence analysis of ITS rDNA was carried out.

2基于多相分类原则，运用生理生化特性、酯酶同工酶酶谱、发酵液挥发性成分GC-MS分析和ITS序列分析的方法，对粒毛盘菌属及其相关类群进行了研究。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。