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Moreover, we we sequenced the entire mitochondrial DNA control region from 17 charadriiform species, and found that the base composition and distribution of the variable nucleotide sites within the control region were similar to other avian species, but a series of short tandem repeated motifs in the 3' end of domain III made A nucleotides and length increased significantly. Phylogenetic analysis showed that these sequences could not well resolve the relationship among genus in Charadriiformes, but within genera maybe has some valuable phylogenetic information.

此外，本研究测定了鸻形目17种鸟类线粒体调控区序列，发现在碱基组成和进化速率上与其它鸟类相似，但其3'末端的串联重复序列导致domain III长度且A含量显著增加；利用调控区序列未能较好的解决鸻形目属以上水平的分类，但对于解决属内物种间的进化关系具有一定的参考价值。

Finally the two phylogenetic trees were compared and the phylogenetic affinity between the 16 species of the genus Chlamydomonas was analysed.

初步尝试以ITS分子序列系统发育分析作为传统性状分析的补充来研究衣藻种间的亲缘关系。

And, we selected some representative species of Collembola and representative groups of Protura, Diplura, Insecta and Arustacean to sequence and analyze their 18S rRNA completely gene sequences, 28S rRNA D6-D7 domain and nearly completely gene sequences to make the studies about the phylogeny relationships of the main families and orders of Collembola, discuss the questions such as the reliability about the new four orders system to Collembola, the monophyletic and ordinal phylogenetic relationships of them and the situation of the special groups, the phylogenetic relationships about Collembola with other groups and the evolution of Hexapod.

同时选取弹尾纲的代表种类和原尾纲、双尾纲、昆虫纲、甲壳动物等节肢动物部分类群，进行了18S rRNA基因全序列和28S rRNA基因D6-D7区以及近全长序列的测定、分析，对弹尾纲重要科属的亲缘关系、进化地位进行分子系统学研究，讨论弹尾纲最新分类系统中四目的确定性、各目单系性、四目的系统关系、特殊类群的分类地位，以及弹尾纲和其它类群之间亲缘关系、六足动物进化等问题。

Three major clusters of numt sequences ( cyt b numt、 ATP8+ATP6 numt and ND4+ tRNA-His+ tRNA-Ser numt) were particularity amplified and sequenced from 10 species within Galliformes and A.galericalata. Comparison numts sequences with homologous mitochondrial partial genes get the different evolutionary characteristics and evolutionary speed compared to mtDNA, then examine the age of insertion of numts using the sequence divergence of numt from the mtDNA. Finally, joined Coturnix japonica、 Coturnix chinensis、 Lophura leucomelana、 Lophura swinhoii and A.albifrons mitochondrial sequences in GenBank, we study the importance function to phylogenetic research of mitochondrial genes and homologous numts.The evolutionary rate of Numts is lower than their authentic sequences of mtDNA, cyt b and ND4 gene evolve faster than homologous numts 2.5 times; ATP8+ATP6 gene evolve faster than numts 18.9 times in Galliformes. Numts show less codon position bias and transition bias under not phylogenetic pressure.

根据2004年3月GenBank登录的红原鸡核基因组草图与其线粒体基因组比对发现红原鸡核基因组中存在3段较长的线粒体假基因，利用PCR方法扩增鸡形目10种鸟类和雁形目中鸳鸯核基因组中3段numt序列：cyt b numt、ATP8及下游ATP6 numt和ND4+tRNA-His+tRNA-Ser numt，测序后分别比较线粒体基因与核同源numt序列的进化特点和进化速率，确定numt序列插入核基因组的时间，以及结合GenBank已登录的日本鹌鹑、蓝胸鹑、黑鹇、蓝鹇和1个雁形目物种——白鹅雁(A.albifrons)相应序列探讨两种同源序列对于鸟类系统进化发育重建的重要作用。

The mitochondrial COⅠ gene sequences of 28 species of Cypraeidae including those of 11 species newly determined were used to reconstruct their phylogenetic trees using the neighbor-joining,maximum parsimony and Bayesian methods,aiming to clarify their phylogenetic relationships.

新测了分布于我国海南沿海的11种宝贝科软体动物的线粒体COⅠ基因序列，结合GenBank中17种该科动物的同源性序列片段，基于不同的数学模型，以邻接法、最大简约法和贝叶斯法构建了宝贝科分子系统树。

The sequence variation analyses showed that there are 236 variable and 221 parsimony-informative in the aligned 605 nucleotide sites, in addition, the relative frequenencies of four nucleotides were not equal an this fact showed that the base compositions were biased. The results of phylogenetic analyses showed:(1) the phylogenetic relationships of the Cypraeidae taxa was basically in consistent with morphology-based ones;(2)Cypraeovulinae was monophyletic in all trees, whereas Nariinae and Cypraeinae were not;(3)the monophylies of Mauritia, Palmadusta, Erosaria, Monetaria, Staphylaea and Pustularia were strongly supported by molecular data;(4)Meyer's speculation that Chelycypraea came out from Luria were also supported.

结果表明：(1)宝贝科28种动物的分子系统树大部分与形态学分类一致；(2)在系统树上，疹贝与鼻贝亚科的其它分类群的距离相隔较远，提示它们之间有较远的亲缘关系，建议将疹贝属列入宝贝亚科；(3)卵宝贝亚科包括希达贝、脊焦贝、筛目贝、拟枣贝、焦掌贝以及呆足贝等共计15个属，它们在系统树上构成独立的一个大支系，构成单系群；(4)支持Meyer将龟甲贝从禄亚贝总属中分离出来的观点，但龟甲贝和林西那贝之间的系统发生关系及其分类地位还有待于进一步探讨。

To study the phylogenetic relationships of Labeoninae fishes in Cyprinidae, the Phylogenetic Tree was reconstructed based on sequences of mitochondrial 16S rRNA.

为研究其在鲤科鱼类中的系统发育关系，以16S rRNA基因作为遗传标记进行鲤科鱼类的系统发育分析。

Lastly, based on analysis on phylogentic relationships among 39 ciliates combined with their main morphologic characters, some conclusion were drawn: 1 the monophyly of the peritrichs was strongly supported, and its subclass rank in the class Oligohymenophorea is reasonable; 2 the genus Epistylis might not be monophyletic, and the peristomial area, especially the peristomial lip, might be an important phylogenetic character within the genus Epistylis; 3 the taxonomic rank of the genus Carchesium and the genus Campanella were suggested to be adjusted; 4 Opisthonecta probably diverged from peritrichs with stalks; 5 the phylogenetic tree of peritrichs was reconstructed based on the molecular information inferred from SSrRNA gene sequences and the morphology.

测定了6科8属13种缘毛类纤毛虫的SSrDNA序列，将构建的最大简约树和邻接树与形态学相结合，分析得出：1）缘毛类纤毛虫是单系的，其分类地位是隶属于纤毛门寡膜纲缘毛亚纲：2）指出累枝虫属可能不是单系的，口围区是累枝虫属中重要的系统发育特征；3）建议调整独缩虫和聚钟虫属的分类地位；4）得出后游虫可能是由有柄缘毛类进化而来的类群；5）基于缘毛类反口区结构和SSrRNA基因序列提供的信息重建了缘毛类纤毛虫的系统发育树。

Moreover, CoI gene was more conservative and a lower evolutionary rate than Cyt b gene. CoI gene was an effective marker in analysing the phylogenesis of families in Passeriformes. It can be used in identifying the species of Passeriformes, but it was revealed that CoI gene was more suitable to identify the phylogenetic relationship of avian family unit than Cyt b gene, and CoI gene can be a molecular marker to identify avian species. But in species identification, CoI gene was less stable and accurate than Cyt b gene. We suggested youd better employ the other marker if you have the CoI gene only.(2) In the phylogenetic trees of birds from Lanius, L.

比较分析了雀形目6科15种鸟类的细胞色素b全序列和CoI基因部分序列，结果显示细胞色素b和CoI基因序列的变异位点分别为454个和366个、简约信息位点为337个和303个，而且线粒体CoI基因比细胞色素b基因略微保守、进化速率也较低；CoI基因在确定雀形目科级阶元之间的系统发生关系方面是一种有效的分子标记，同时它也能够用于雀形目鸟类的物种鉴定，但在物种鉴别方面不如细胞色素b基因稳定、准确。

Furthermore, out of 497 fAFLP markers, 80 special bands were found to be able to distinguish the four groups from each other and may be applied for germplasm characterization and molecular assistant classification of Meretrix clam.4 Molecular classification of two species of Meretrix clam based on fAFLP and ITS sequences4.1 The results of fAFLP maker analysis of S, G and W showed that each group had their own specific loci among which there were 53 special loci in W group, much more than those of S group (14) and G group (21). Among the 53 loci, nine were all dominant loci. These unique loci could be taken as molecular markers to distinguish W from other groups. The genetic similarity indexes and distance matrix between S and G groups were 0.9585 and 0.0424 respectively, but the genetic similarity indexes and distance matrix between W group and S or G group was 0.7939 or 0.7941, and 0.2308 or 0.2305 respectively. The results revealed that significant difference existed between W and S or G groups in molecular genetic structure. The phylogenetic trees by the methods of UPGMA and NJ also indicated that S and G populations were very closely related, while W population was a relatively independent cluster, lying beyond the species which S and G belong to.4.2 The internal transcribed spacer region of the rDNA from S group, G group and W group were PCR amplified and sequenced. The results showed that the size of ITS ranged between 1266-1269bp in W group, while those in G and S groups were 1614bp and 1520bp respectively. The GC content ranged 62.32-62.62% in W group while it was 61.77% in G group. The genetic distances between three populations of W group were 0.001～0.003, but it was 0.110 or 0.147 respectively between W group and G group or S group. Phylogenetic trees by NJ method also showed that G group was very closely related to S group, while W group was a relatively independent cluster.

在457个总扩增位点中找出了53个W的特有位点，远多于S群体(14)和G(21)群体，而且在53个特有位点中有9个出现频率为100%的位点，这些位点可以作为区分其它2个群体的特征性标记；S– G群体特有的位点有112个，其中有4个位点出现频率为100%，可作为S– G群体区别于W群体的特征性标记。S群体和G群体间的遗传相似性系数为0.9585，遗传距离只有0.0424，在NJ和UPGMA法构建的亲缘关系的树状图上均首先聚在一起，说明二者的亲缘关系很近，应属于种内群体间的关系；而W与S和G的遗传相似性系数均较小(0.7939和0.7941)，相对遗传距离很大而且十分相近(0.2308和0.2305)，在亲缘关系树状图上单独分出一支，也表明W与S和G群体间的亲缘关系较远。4.2 ITS序列比较分析通过对白壳文蛤、山东文蛤和广西文蛤的ITS序列扩增电泳、PCR-RFLP分析和ITS序列分析发现，W的ITS序列长度在1266-1269 bp，而S和与G的ITS序列总长度分别为1520 bp和1614 bp；从ITS1和ITS2长度来看，W分别为739-741 bp和316-317 bp，S为895 bp和414 bp，G为987 bp和416 bp；而从ITS碱基组成来看，W的GC含量在62.32-62.62%之间，而G群体为61.77%。W的3个壳色不同群体间的遗传距离仅0.001、0.002和0.003，S与G群体间的遗传距离是0.010，说明W群体内变异很小，而S与G群体间已出现明显的遗传分化，但还均属于种内群体间的遗传变异；而W与G和S的遗传距离分别达到0.110、0.147，两个类群差异显著，已远超出种内群体间的遗传变异。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。