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For exploiting and making use of rhizobium species resources of planting leguminous trees in south China,the full_length 16S rDNA sequences of representative strains HJ06 and JJ06 of two rhizobia,isolated from Acacia crassicapa and A.cincinnata in south China,were determined.These sequences were compared with all known rhizobia and related bacteria and an unrooted phylogenetic tree was produced.

为了开发利用我国人工林豆科树种根瘤菌的种质资源，该研究对华南地区两个人工林豆科树种厚荚相思和卷荚相思的根瘤菌两个代表菌株HJ06和JJ06进行了16S rDNA全序列测定，将此全序列与根瘤菌各已知种及相关种的16S rDNA全序列进行了比较及聚类分析，得到系统发育树状图。

And unrooted trees were established. The accession numbers in GenBank of the sequences of 16S rDNA of the strains were followed by PFK1(DQ295034), PFK2(DQ295035), PFK3(DQ295036), LZCL(DQ295040), ZCS1(DQ295037), MMA1(DQ295041), MMA2(DQ295038), JSQ1(DQ295042), JSQ2(DQ295039), JSQ3(DQ295043). The Blast and phylogenetic tree analysis showed that

3将分离菌株进行形态、生理和糖发酵实验，结果表明这些表型鉴定的结论与16 S rRNA基因序列同源性分析结论一致，分离纯化5种产品的10株菌中仍有相同的3株菌与标签标注的不一致，即其中标签上分别标注为青春双歧杆菌和长双歧杆菌的两株菌鉴定结果均为动物双歧杆菌

Phylogenetic analysis showed that G7-3, G31-1-5 and G31-1-10 were in the branch of Rhizobium, F7-2-11 was in the branch of Bradyrhizobium, DH001, LH001, LL007 acid, Y wood 1 and F3-2-6 were in the branch of Mesorhizobium and H1-1-1 was in the branch of Paenibacillus sp.

系统发育学分析表明：G7-3、G31-1-5、G31-1-10位于根瘤菌属分支中；F7-2-11位于慢生根瘤菌属分支；DH001、LH001、LL007酸、Y木1、F3-2-6位于Mesorhizobium分支中；H1-1-1属于类芽孢杆菌(Paenibacillus sp。)。

In the phylogenetic tree, the cattle and zebu were clustered together first, followed by the yak, buffalo, pig, human, mouse, and rat.

HSL基因外显子Ⅰ部分核苷酸序列进行多序列对位排列构建的各物种间分子系统进化树结果表明，普通牛和瘤牛首先聚为一类，再分别与牦牛、水牛、猪、人聚类，最后与大鼠、小鼠聚为一类。

The result shows that firstly,zebu ismost closely related to cattle;secondly,closely related to yak,buffalo,pig,human,lastly,related to rat and mouse.The results of the phylogenetic trees reflect the molecularevolution relationship among these species,consistent with the zoological classification.The genetic distances among yak,cattle and zebu were relatively small,but geneticdistances between these three species and buffalo were relatively large.

研究表明，牦牛、普通牛和瘤牛三个物种间的遗传距离远小于它们各自与水牛这一物种的遗传距离，三者之间的亲缘关系也相对于它们与水牛间的亲缘关系较近，故将牦牛、普通牛和瘤牛划分在同一个属—牛属，而将水牛划分在另一个属更为合理。

The partial sequences of exon Ⅰ of hormone-sensitive lipase genes in yak, cattle, zebu, and buffalo were analyzed. Comparisons of these sequences and the deduced amino acid sequences with the homologous HSL gene and protein sequences in other mammalian species including pig, human, mouse, and rat retrieved from the GenBank were carried out and finally a phylogenetic tree was constructed using the partial DNA sequences of the HSL genes in all species.

在对牛科中4种动物即牦牛、瘤牛、普通牛和水牛HSL基因外显子Ⅰ部分核苷酸序列进行测定的基础上，与Gen- Bank中其他物种相应基因核苷酸序列、氨基酸序列进行了比对分析，并构建了牦牛与其他物种间分子系统进化树。

Descriptions of single new species may be accepted if accompanied by significant information on zoogeography, ecology, phylogenetic relationships, or other biological concerns.

如含有在动物地理、生态学、系统发生关系或在生物学其它方面具有显著意义的信息，可接受有关单个新种的简短描述。

Phylogenetic analysis showed that sugarcane was firstly clustered with maize, then with sorghum and Zoysia japonica, and then with rice.

该基因片段具有其它植物GA20氧化酶基因中存在的保守区；同时，与多种单子叶植物的GA20氧化酶基因的核苷酸和氨基酸序列的同源性在80％和75％以上。

Seven major bacterial isolates were obtained and purified. Their 16S rRNA genes were amplified by PCR for sequencing and their identities were determined with homology comparisons. Five of the seven isolates belong to Actinomycetales including Kocuria KD139, Rhodococcus KD140, Rhodococcus KD142, Arthrobacter KD230, and Arthrobacter KD232; one is classified as Bacillus d KD178; and another one as Stenotrophomonas KD237. The phylogenetic tree was also constructed based on the analysis of 16S rRNA gene sequences.

通过对分离菌株的16S rRNA基因序列进行分析，发现其中5株细菌分别属于放线菌目的考克氏菌属(KD139)、红球菌属(KD140和KD142)和节杆菌属(KD230和KD232)， 1株细菌属于杆菌目的芽胞杆菌d属(KD178)，另外1株细菌属于黄色单孢菌目的寡食单胞菌属(KD237)；同时我们构建了系统进化树，确定分离菌株的相对进化地位。

Sequence comparison shows that MuMADS2 belongs to the AGAMOUS subfamily. Furthermore, phylogenetic analysis suggests that MuMADS2 is most likely a class DMADS-box gene.

将MuMADS2与其它植物来源的MADS-box基因进行序列比较发现，MuMADS2属于MADS-box基因的AGAMOUS亚族的D世系。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。