查询词典 phosphatase

Consequently, the enzymatic hydrolyzable P may contribute to the internal loading of the eutrophic lake, and stratification of kinetics and compositions of phosphatase in shallow lake is theoretically noticeable.

因此，富营养湖泊沉积物中磷的&内负荷&应有可酶解有机磷的含义，浅水湖泊中磷酸酶的活性组成和动力学参数的分层现象当为湖沼学研究中值得深入探讨的新理论问题。

Detect that whether the ADSCs were differentiated inducedly into osteoblasts or adipogenic cells by Alkaline phosphatase staining、Von kossa staining and Oil red "O"staining after the ADSCs were cultured in inductive basic medium containing osteogenic induction agent and adipogenic inducers for four weeks.②.

①。采用密度梯度离心法加贴壁培养法对兔脂肪组织进行了分离纯化，CD44免疫组化法分析ADSCs表面标志；用含成骨、成脂诱导培养基培养4周后，行碱性磷酸酶染色、Von kossa染色及油红&O&染色检查ADSCs是否向成骨、成脂细胞定向分化。②。

Bone marrow mesenchymal stem cells could differentiate into osteoblasts, adipocytes and neural like cells with osteoblast inductor (β-sodium glycerophosphate, dexamethasone, vitamin C), lipoblast inductor (dexamethasone, 3-isobutyl-1-methylxanthine, bovine insulin, indometacin) and serum-free medium inductor (dimethyl sulphoxide, butylated hydroxyanisole) respectively. Osteoblast marker (alkaline phosphatase, osteocalcin mRNA, calcium node), adipocyte marker (lipid droplet, PPAR γ-2mRNA) and neural cell-like marker (nissl body, neuron specific enolase, neurofilament protein) were respectively determined by the immunohistochemical method, polymerase chain reaction and immunocytochemical method.

分别采用成骨细胞诱导剂（β-甘油磷酸钠，地塞米松，维生素C）、成脂肪细胞诱导液（地塞米松，甲基异丁酸黄嘌呤，牛胰岛素，吲哚美辛）及二甲基亚砜和羟基丁酸苯甲醚无血清培养基诱导剂干预细胞向成骨、脂肪、神经细胞分化，经免疫组织化学染色、PCR、免疫细胞染色方法检测成骨标志物（碱性磷酸酶、骨钙素mRNA、钙结节）、脂肪标志物（脂滴、PPARγ-2mRNA）、以及类神经标志物（尼克氏体、神经烯醇化酶、神经丝蛋白）。

The cells were identified by invert phase contrast microscopy and alkaline phosphatase staining and mineralized nodules staining.The activity distribution of osteoblasts was determined by microtitration during a culture cycle.

通过倒置相差显微镜观察，并用碱性磷酸酶和矿化结节染色鉴定，利用微量滴定法测定一个培养周期的成骨细胞活性分布，探讨一种经济、高效的原代成骨细胞培养方法。

In addition of cytomorphologic identification, immunolhistochemic technique of bone morphogenic protein-monoclonal antibody, alkaline Phosphatase staining and concentration assays, as well as osteoblasts reaction to PTH-cAMP were used, according to the biologic character of osteoblasts.

除了在细胞形态学上对其鉴定外，还根据成骨细胞的生物学特性，首次引用了骨形成蛋白单克隆抗体免疫细胞化学技术，以及碱性磷酸酶染色和含量测定，成骨细胞对甲状旁腺激素-cAMP反应等方法，验证了经酶消化分离培养的兔颅骨细胞具有成骨细胞的功能和特性。

AIM: To examine the role of matrix metalloproteinases expressed by the tartrate-resistant acid phosphatase-positive mononuclear and multinucleated cells in articular cartilage damage.

目的：观察在胶原诱导的C57BL/6小鼠类风湿性关节炎模型中，基质金属蛋白酶-2、9在耐酒石酸盐酸性磷酸酶阳性的单个核及多核细胞中的表达及其在关节软骨损伤中的作用。

The influences of some metal ions in seawater treated by Cephlex 100 on metamorphosis, growth and activities of alkaline phosphatase and ATPase in mysis and postlarvae of Chinese prawn Penaeus chinensis were studied.

向经过螯合剂Cephlex 10 0处理的海水中添加金属离子，进行培养中国对虾糠虾幼体和仔虾幼体，观察金属离子对变态发育及其体内碱性磷酸酶和ATPase活性的影响。

By means of the electron microscopic enzymo cytochemistry, it was observed that the activity of acid phosphatase was localized on the autophagic vacuole, the enzyme reaction granules gather to form the vacuolar bodies of various shapes in the cytoplasmic region of the osmiophilic and paraglycogen granules in resting Stylonychia mytilus .

该文应用电镜酶细胞化学方法显示，贻贝棘尾虫休眠包囊中，在胞质嗜锇粒和拟糖原粒区，酸性磷酸酶反应颗粒聚集于具有消化泡作用的泡状体上，葡萄糖 6 磷酸酶反应也定位在自噬泡膜位置。

Serum indicators-urea nitrogen, cholesterol total, alkaline phosphatase manifestly were heightened and some animals' hepatic cells, nephric tubules and mucosa emerged differently damage at histomorphology by giving crude high dose after 2 months.

连续给药2个月，可见生品的高剂量组的血清生化指标的尿素氮、总胆固醇和碱性磷酸酶明显升高，组织形态学检查部分动物的肝细胞、肾小管和胃黏膜均产生程度不同的损伤。

Differentiation of osteoblasts was evaluated by detecting the activity of alkaline phosphatase using 4-nitrophenyl phosphate dsodium salt method and by measuring the mineralized nodiform dyed by alizarin bordeaux.

利用基因克隆和报告基因方法，检测槐角苷和染料木素对人骨保护素(osteoprotegerin， OPG)启动子活性的作用，观察两者对骨保护素基因转录活性的调节。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。