查询词典 phosphatase

Identification of these cells: Over 90% cells isolated from human fetal calvaria were stained positively for both alkaline phosphatase and Alizarin red.

采用酶消化后植块的改良植块培养法可在较短时间内获得大量成骨细胞，这些细胞具有典型的成骨细胞形态和功能，是一种较好的分离培养人胚成骨细胞的方法。

The activities of succinate dehydrogenase in hepatocytes, alkaline phosphatase and thiamine pyrophosphatase on the wall of bile canaliculi became obviously less.Both the activities and localizations of TPPase had changed. Some TPPase from the damaged lysosome-like vesicles and Golgi saccules of hepatocytes discharged into the cytoplasm. TPPase reaction product in some bile canaliculi decreased.

酶细胞化学技术显示肝细胞线粒体琥珀酸脱氢酶和毛细胆管壁上的碱性磷酸酶活性明显减弱或消失，焦磷酸硫胺素酶的活性及定位也发生了改变， TPPase从损伤的高尔基复合体及溶酶体样小泡漏出进入胞浆，部分毛细胆管壁上的TPPase反应物减少。

Acid phosphatase activity in the rhizosphere of Castanopsis sclerophylla varied sharply with the size of an island, but was not significantly related to AM infection rate.

苦槠根际土壤酸性磷酸酶活性在中岛、大岛与陆地间差异显著，但与AM侵染率之间无显著相关性。

Glucose-6-phosphatase system (G6PA) is the key enzyme that catalyses the reaction glucose production in cells, which is composed by 5-6 subunits.

葡萄糖-6-磷酸酶（G6PA）是催化细胞内产生葡萄糖的关键酶，此酶由5-6个亚基构成。

Tissue composition analysis, nutrient enrichment and starvation treatments, bioassay and in situ extracellular alkaline phosphatase activity determination showed that growth of Sargassum duplicatum, Codium edule and Ulva latuca were limited by phosphorus during the early growth period while nitrogen during the fast growth period. Growth of Enteromorpha linza, Gracilaria coronopifolia and Laurencia papillosa were limited by phosphorus.

南湾及眺石海水优养化可能是造成大型海藻单位面积生物量明显增加的原因，藻体营养分析、室内营养盐添加与不添加处理、bioassay及insitu 细胞外磷酸分解酵素活性证明重缘叶马尾藻、食用松藻及大石莼的营养盐限制因子在初期是磷而快速生长期是氮而浒苔、伞房龙须菜及乳头凹顶藻是磷。

To improve detection efficiency and result accuracy, we developed an improved multiplex PCR technique for specific detection of GMO.①The assay strategy involves multiplex PCR amplification of the target with several biotinylated primer pairs in one tube, followed by the hybridization of multiplex PCR products and membrane-bound oligonucleotide probes. The multiplex PCR/probe hybrid is detected colorimetrically using an alkaline phosphatase conjugate and a chromogenic substrate.

在多基因定性检测技术方面，本文在优化并确定了多基因PCR反应条件的基础上，采用了两种方法对多基因PCR产物进行鉴定：①采用共价结合方法将多个探针固定在Biodyne〓C膜上，将5'端标记生物素的多基因PCR产物与膜上的探针进行杂交及显色反应，首次建立了GMO的多基因PCR-膜相杂交检测方法，实现了一个反应体系中同时检测多种转基因成分的目标，提高了检测效率与准确率。

This paper reviewed its pathogenesis, forms, clinical manifestations, differential diagnosis, treatment and prognosis. Here a case of neonatal hypophosphatasia is reported. This baby was female (30 minutes old). Prenatal ultrasound showed disproportionate biparietal diameter and long bones of limbs in the baby. After birth, she presented with obvious craniomalacia, respiratory distress and cyanosis. Serum alkaline phosphatase level was significantly reduced. Both X-ray and autopsy showed extremely insufficient skeletal mineralization. Four days later she died of respiratory failure.

该文对其发病机制、分型及临床表现、鉴别诊断、治疗和预后进行了综述，并介绍了在该院诊断明确的1例新生儿型的罕见病例，患儿为出生30 min女婴，产前B超提示胎儿双顶径与四肢长骨不成比例，生后即有明显的颅骨软化、呼吸困难和紫绀等表现，血碱性磷酸酶显著低下，X线表现及尸检结果均提示骨骼矿化极度低下，4 d后因呼吸衰竭死亡。

The histochemistry staining of cytokeratin, vimentin, alkaline phosphatase were positive and that of α-actin,Ⅷ Ag, fibronectin and desmine were negative.

细胞角蛋白、波形蛋白和碱性磷酸酶染色阳性，α-肌动蛋白、Ⅷ因子相关抗原、纤维连接蛋白和结蛋白染色阴性。

Cytokeratin, vimentin, alkaline phosphatase,α-actin,Ⅷ Ag, fibronectin and desmine were determined using the third generation cells. The ultrastructure were examinated by the transmitted and scanning electronic microscope.

取第三代细胞检测细胞角蛋白、波形蛋白、碱性磷酸酶、结蛋白、Ⅷ因子、α-肌动蛋白的表达，应用扫描电镜和透射电镜观察细胞的超微结构。

Objective To establish a new method to measure alkaline phosphatase activity using disodium p-acetylphenylphosphate (PAP-PNa2) as substrate.

目的 合成对乙酰基苯基磷酸二钠盐(PAP-PNa2)，并建立以PAP-PNa2为底物的碱性磷酸酶测定方法。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。