查询词典 phosphatase

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

In the first section, the effect of sodium houttuyfonate on the level of lysozyme secreted by macrophages was first tested by bacteriolytic method; then the effect of sodium houttuyfonate on the level of tartaric acid resistant acid phosphatase produced in macrophages was investigated by analyzing the phenol generated in the reaction that was catalyzed by the enzyme; the third, the effect of sodium houttuyfonate on the phagocytic activity of macrophages was observed by microscopy; the forth, the effect of sodium houttuyfonate on the production of complement C4 from macrophages was tested by hemolytic method; the fifth, the effect of sodium houttuyfonate on the expression of C3b receptors on the surface of macrophages was analyzed by observing the formation percentage of zymosan wreath; the sixth, the effect of sodium houttuyfonate on the respiratory burst in macrophages was tested by flow cytometry; the seventh, the effect of sodium houttuyfonate on the production of IL-1α and IL-1β from macrophages was tested by ELISA method.

在这一章中，首先，采用溶菌法考察合成鱼腥草素对巨噬细胞分泌溶菌酶水平的影响；其次，采用游离酚法研究合成鱼腥草素对巨噬细胞产生的耐酒石酸酸性磷酸酶水平的影响；第三，采用显微镜观察法考察合成鱼腥草素对巨噬细胞吞噬功能的影响；第四，采用溶血法测定合成鱼腥草素对巨噬细胞产生的补体C4水平的影响；第五，采用酵母花环法检测合成鱼腥草素对巨噬细胞表面C3b受体水平的影响；第六，采用流式细胞术法观察合成鱼腥草素对巨噬细胞呼吸爆发的影响；第七，采用ELISA法探讨合成鱼腥草素对巨噬细胞产生IL-1α及IL-1β水平的影响。

Methods To determine the serum PCT of 35 patients with bacteriosis and 15 patients with nonbacterial disease by gold immunochromatographic assay,and determine C-reactive protein and alkaline phosphatase of neutrophilic granulocyte in corresponding period.

方法采用半定量的胶体金免疫结合法，测定35例细菌感染性疾病住院患者及15例非细菌感染性疾病住院患者的PCT，并同期测定患者C-反应蛋白与中性粒细胞碱性磷酸酶。

The laboratory findings of a typical CCML patient comprised of peripheral blood leukocytosis, basophilia and eosinophilia,myeloid differentiation in different stages, and increased megakaryocytes. The immunohistochemical features of the CCML consisted of highly positive MPO and CD68, significant lowering of neutrophil alkaline phosphatase, positive for Philadel- phia chromosome or chimeric BCR/ABL gene, etc. But in most cases of juvenile CCML, the Philadelphia chromosome could not be detected.

临床表现以乏力、低热、贫血、肝脾及淋巴结肿大为主要特点；典型儿童慢性髓细胞白血病的实验室特点有白细胞计数高、嗜酸和嗜碱性粒细胞增多、骨髓不同阶段髓细胞分化和巨核细胞增多；免疫组化提示CD68，髓过氧化物酶，中性粒细胞碱性磷酸酶积分明显减低，Ph染色体或BCR/ABL融合基因阳性，而幼年型CCML的Ph染色体常为阴性。

As a kind of potential drug for treating the type Ⅱ diabetes, the inhibitor of protein tyrosine phosphatase 1B (PTP1B) is an important research field, in which a kind of bidentate inhibitors is the research focus.

作为二型糖尿病的潜在治疗药物，蛋白质酪氨酸磷酸酯酶1B(PTP1B)的抑制剂研究一直备受关注。

As a kind of potential drug for treating the Type II diabetes, the inhibitor of protein tyrosine phosphatase 1B (PTP1B) is an important research field, in which a kind of bidentate inhibitors is the research focus.

中国科学院研究生院，北京 100049摘要：作为二型糖尿病的潜在治疗药物，蛋白质酪氨酸磷酸酯酶1B（PTP1B）的抑制剂研究一直备受关注。

Some patients had suffered from obstructive jaundice, pancreatitis, bilirubinemia, alkaline phosphatase raise,γ-glutamyl transpeptidase raise and/or diameter of common bile duct exceeded 0.8 cm; and for these cases intraoperative cholangiography must be carried out. 405 cases needed T-tube insertion. The common bile duct of 105 cases was sutured without T tube. Results All cases were operated successfully.

术前确诊者，术中直接行胆总管切开胆道镜取石；术前有黄疸史、胰腺炎史和直接胆红素增高、胆系酶增高者，或胆总管在0.8 cm以上者行术中造影，明确有胆总管结石的切开胆总管胆道镜取石。405例置T管引流，105例行胆总管Ⅰ期缝合。

Five missense mutations in the most commonly altered PTP were biochemically examined and found to reduce phosphatase activity.

在人类肿瘤细胞中表达野生型而不是突变型PTPRT可以抑制肿瘤生长。

Acid phosphatase from Branchiostoma beleheri was isolated and purified by ammonium sulfate fractionation,Sephadex G-75 gel filtration and CM-cellulose chromatography under acid condition.

从厦门文昌鱼Branchiostoma belcheri分离提纯酸性磷酯酶(EC3.1，3.2)，进一步经Sephadex G-75和羧甲基纤维素(CM-52)柱层析纯化，获得聚丙烯酰胺凝胶电泳单一纯酶制剂，比活力为351μm/min mg。

The results show, under the long-trem influence of different farming behaviors, the soil, collected from dry-land in low multiple crop index to dry-land in high multiple crop index to composite-land in high multiple crop index, decease in bult density, clay content, total P and activities of catalaes and increase in organic matter, humic acid contents, nitogen, available nitrogen, phosphorus, potassium, soi breathe intensity and urease, phosphatase, protease active content.

选择贵州中部喀斯特山区普定县猫洞小流域地区，通过长期受不同生产行为影响的区域（低复种旱作区、高复种旱作区、高复种复合农作区）的土壤采样分析，结果表明：喀斯特山区小流域土壤在长期不同生产行为影响下，从低复种指数旱作区、高复种指数旱作区到高复种指数复合农作区，土壤容重、粘粒含量减小，而土壤有机质、腐殖酸含量、全氮、碱解氮、速效磷、速效钾、土壤呼吸强度、脲酶、磷酸酶、蛋白酶活性含量依次增大，土壤全钾含量和过氧化氢酶活性却依次减小。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。