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The recoveries for 2,3-BD and acetoin were 91%and 92%,respectively.Aqueous two-phase extraction of 2,3-BD from real fermentation broth,the removal ratio of cells was above 99%,and the soluble proteins were about 85%in the top phase,which resulted in easy recovery of 2,3-BD from the top phase by vaporization because a viscous slurry was not formed by glucose,cells and soluble proteins at high temperature as in traditional separation methods.In addition,the cells recovered from the fermentation broth could be used as inoculum for further fermentation.

结果表明,目标产物2,3-丁二醇和乙偶姻主要富集在上相中,而葡萄糖主要富集在下相中;当异丙醇和硫酸铵的质量浓度分别是34%和20%时,2,3-丁二醇和乙偶姻的分配系数分别为8.29和8.98,回收率分别达到91%和92%;采用该条件对发酵液直接进行萃取,细胞的去除率在99%以上,可溶性蛋白去除率可达85%。

Therefore,K. pneumoniae CICC 10011 is a potential strain for 2,3-BD production.Thirdly,a novel aqueous two-phase system for the recovery of 2,3-BD from fermentation broth was developed employing isopropanol/(NH_4)_2SO_4 system.The results showed that most of target products(2,3-BD and acetoin) can be partitioned efficiently into the top phase,while most of glucose can be partitioned into the bottom phase.

通过对批式流加发酵过程中两株克雷伯氏菌代谢流量的分析表明,K.pneumoniaeCICC 10011代谢产生的各种有机酸和乙醇浓度均明显低于K.pneumoniae DSM 2026,而目标产物浓度以及产物转化率却相对较高,是发酵2,3-丁二醇的优良菌株。

The results showed that : three species all can resistant to drought ,wind-drift sand and endure to sterile soil, and with fine adaptability to local condition that there are irrigation in growth phase and no irrigation in post-vegetative phase in 2002 and there are no irrigation in growing period and irrigation in after bearing phase in 2003. Among the three species ,Agropyron cristatum× A.desertorum cv.

结果表明,在2002年生长期有灌溉和果后营养期无灌溉与2003年生长期无灌溉和果后营养期有灌溉的条件下,3种冰草表现出抗旱、抗寒、耐瘠薄的特点,对当地条件具有较好的适应性。

Gas phase method contained titanium tetrachloride gas phase method, vapor-phase hydrolysis of titanium alkoxide method, evaporation of Low Pressure Gas method, and so on.

液相法中的几种方法由于工艺简单、条件温和,气相法反应速度快,过程易放大,容易实现连续生产。

Dr. Li Song laughs at character: Amative process of the men and women can be divided for two phase, the first phase is the target that finds appointment as soon as possible, the 2nd phase just is the romantic process of wind,flowers,snow and moon.

李松博士笑言:男女的恋爱过程可以分为两个阶段,第一个阶段是尽快找到约会的对象,第二个阶段才是风花雪月的浪漫过程。

The X-ray diffraction patterns were calculated analogically by using Rietveld method and the phase content in rare-earth two phase Nd2Fe14B/α-Fe magnet was determined based on standardless quantitative phase analysis.

稀土双相永磁合金是基于软磁相的高饱和磁化强度和硬磁相的高各向异性的特点,通过两相晶粒在纳米尺度上交换耦合而成的一种纳米晶复合永磁材料,它具有显著的剩磁增强效应,可望获得较高的综合磁性能[1~3]。

The Phase I tip has simple straight swept or tapered shape, the curve change of the platform is included during Phase Ⅱ, and the geometry of the Phase Ⅲ tip is extended to anhedral modification.

第一层次的桨尖形状为直线后掠或尖削,第二层次包括桨尖平面的曲线变化,而第三层次进入到下反桨尖这种三维变化。

The experimental results show that: lithium is scatteredly precipitated as natural lithium carbonate in the whole evaporation process, and the highest content of natural lithium carbonate in solid phase is 2.0%, so it is difficult to get the mixed salt with high-grade lithium carbonate; boron is precipitated as borax in the whole evaporation process, and borax is precipitated in large quantity with the highest content of 15.64% in solid phase until the brine is extremely concentrated (CWR≥97.9%) and intergrows closely with typical cold-phase natron; potassium is precipitated as sylvite and aphthitalite in the whole evaporation process with a content from 9.49% to 34.44%.

结果表明:在低温蒸发状态下,锂以天然碳酸锂形式在整个蒸发过程中分散沉淀,固相最高含量仅达2.0%,因此难以获得较高品位的富锂混盐;硼在全蒸发过程中以硼砂形式自然沉淀。当卤水高度浓缩(蒸失率达97.9%以上)时大量析出并与典型冷相矿物泡碱紧密共生,固相中硼砂含量最高可达15.64%;钾以氯化钾和钾芒硝形式沉淀,在蒸发浓缩的全过程中均有不同程度析出,固相含量在9.49%~34.44%之间波动。

The growth curve showed proliferative phase 3~8th days, top-plate phase 9~10th days and attenuative phase after 10th days.

细胞生长曲线,增殖期3~8天,平顶期9~10天,衰亡期10天以后。

The growth curve showed proliferative phase 3~8th days, top-plate phase 9~10th days and attenuative phase after 10th days.

细胞生长曲ˇˇ增殖期3~8天ˇ平顶期9~10天ˇ衰亡期10天以后。

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