查询词典 phase transformation

Three donor wheat all show good character and had little genotypical difference in the aspect of inducement、differentiation、regeneration etc. They could all nice genotypical cultivars for wheat transformation. 3160 embryos have been used to transformation (960 embryos belongs to Atlas66, 1000 embryos belongs to EM12, 1200 embryos belongs to Scout66 respectively), At last, we got twenty-six of which were positive cultivars through PCR. The preliminary whole transformation frequency was 0.82 percent. Also the citrate synthase activities of the leaves of T_0 progeny show 5～9 higher than the control lines.

转化过程中分别使用了960个Atlas66、1000个EM12和1200个Scout 66幼胚(总记3160个)，经抗性筛选、CS与标记基因的PCR检测初步表明有26株为阳性植株，总转化率为0.82%；同时进一步对T_0代植株叶片柠檬酸合酶以及T_1代植株经铝胁迫后对其根尖分泌物进行检测，结果在上述转化植株中检测到了柠檬酸合酶的活性，且转化材料比对照材料平均高出了5～9倍；同时铝胁迫后，T_1植株根尖检测到了苹果酸及柠檬酸的分泌。

Results When the culture medium was supplemented with 0 .05μg/ml sodium selenite,the proliferation of transformed cells was significant ly promoted and the transformation did not change remarkably,when supplemented w ith 0.25μg/ml,the proliferation was promoted but the transformation was inh ibited,and when with 0.75μg/ml and 1.50μg/ml,the proliferation was not pro moted and the transformation was strongly inhibited.Big dose of (1.50μg/ml) sodium selenite m ade the nuclei of the untransformed lymphocytes gather together like a few grape s doing,and even caused them to fuse.

结果 在培养物中添加0.05μg/ml亚硒酸钠能显著促进转化细胞增殖；添加0.25μg/ml可促进转化细胞增殖，但显著抑制细胞转化；添加0.75μg/ml和1.50μg/ml，不仅不能促进细胞增殖，而且强烈抑制细胞转化。1.50μg/ml剂量的亚硒酸钠使非转化淋巴细胞发生葡萄状聚集甚至核融合。

Of value judgment and in the aspect of concrete ethical norms, thoughts of modern nationality transformation embodied the modern transformation from the three cardinal guides and the five constant virtues as specified in the feudal ethical code to the bourgoise morality of independence, freedomand equality,and finally to the socialist code of unity, mutual aid and coorperation, and furnished the concrete standards of conduct.On one hand, on the basis of modern transformation of social tendency of value judgement and moral norm, China modern bourgeois thinkers broke the feudal shackles in people's mind by attacking fierely such backward psychology as back to ancient , conservatism, passivity, nonaction, closing the country

在社会价值取向和道德规范近代转换的基础上，主张国民性改造的近代资产阶级思想家猛烈批判了复古、保守、消极萎缩、无为不争、闭关自守、虚矫伪饰以及被动依附的落后心理，打破了人们心灵的封建枷锁；另一方面，他们又通过多种方式大力弘扬重今、创新、竞争奋进、开放、务实、自主等近代社会心理，把个人主义、社会主义的价值原则和自由、平等、独立的近代道德规范转化为人们的心理自觉，为实现人们行为方式的近代转型打下了扎实的基础。

The catalytic transformation pathway can change to decrease the yield of hydrogen and yield methyl-contained sulfides such as methyl mercaptan, dimethyl sulfide and dimethyl disulfide, but overlarge concentration of methanol would holdback the transformation of isobutyl mercaptan. The transformation of isobutyl mercaptan was favorable if the volume ratio of methanol and benzene was 0.2.The addition of methanol assume certain effect on the catalytic cracking of di-n-butyl sulfide in solvent benzene but without changing the cracking reaction mechanism of di-n-butyl sulfide.

甲醇也可以使异丁硫醇的催化转化路径发生改变降低硫化氢的产率，生成甲硫醇、甲硫醚及二甲基二硫化物等含甲基硫化物，但甲醇的浓度过高会抑制异丁硫醇的转化，在反应体系中当甲醇的体积占到溶剂苯体积的20%时对异丁硫醇的转化较为有利；甲醇的添加对丁硫醚在溶剂苯中的催化裂化有一定影响，但没有改变丁硫醚的裂解反应历程。

Pisang Mas as transformation receptor, and optimized the genetic transformation system of banana from aspects of hygromycin selection concentration and selection method in order to establish a high efficient Agrobacterium tumefaciens-mediated genetic transformation system for banana.

为建立根癌农杆菌介导的香蕉高效遗传转化系统，本研究以雄性花诱导产生的贡蕉胚性悬浮系为转化受体，从潮霉素筛选浓度和筛选方法两方面进行了优化。

After having established genetic transformation system with tomato cotyledons as explant and determined the transformable of preculture time,incubation time and co-culture time,we set up the system of high frequency transformation of tomato cotyledons.Then hBMP-3m gene was transferred into tomato via Agrobacterium-mQdiated transformation,and the resistant plants to Hyg were obtained.

建立了以番茄子叶为外植体的遗传转化体系，确定了预培养时间、浸菌时间及共培养时间等转化条件，建立了番茄高频转化系统，然后通过农杆菌介导法将hBMP-3m基因导入番茄，并且获得了Hyg抗性植株。

The results showed that 7-day-precultured embryogenic calli had the best responses for bombardment among 2-7 day' preculture duration. Enhanced transformation efficiency was found in the explants treated with high osmotic medium 4h prior to till 24 h after bombardment. Particles of gold and tungsten were both found suitable for rice transformation and there was no adverse effect when tungsten particles were reduced to 1/3 standard amount for DNA precipitation on transient GUS gene expression. However, it was found that gold particle had higher GUS expression at 120 h post-bombardment. A preliminary microprojectile transformation system with an average of 15-20 blue spots/explant GUS expression was routinely obtained for 'Tainung 67' by using the particle gun with 1,100 psi rupture disk, 6 or 9 cm microcarrier flight distance and 4 mm of macrocarrier flight distance setting.

初步结果显示，以预培养2~7日之胚性癒合组织逐日测试转殖率，以预培养7日之培植体表现最佳；培植体於转殖前4小时至转殖后24小时以高渗透压培养基处理，有助於转殖效率之提高；比较金、钨粒子对转殖率在转殖后48h並无不同，但转殖后120 h取样，则金粒子组有较高之蓝点数；钨粒子用量可减少至1/3標准用量对转殖率並无影响；利用基因鎗在使用1， 100 psi裂碟片、大载片至挡片之距离为4 mm、微载髁至射击目標之飞行距离6或9 cm之设定，GUS基因表现之平均蓝点数均可达15~20个／培植体。

The protein had the same antigenicity as IgG, and the change of the protein had the same tendency as SHP-2, which was higher in canceration groups than in uncanceration groups and control groups in thymus cells, but there weren't significant change in bone marrow cells;⑧ The change law of the expression of SHP-1 and SHP-2 during γ-ray induced malignant transformation in BEAS-2B cell is that the expression of SHP-1 was increased synchronistically with malignant transformation of cell, and the expression of SHP-2 didn't have significant correlation with malignant transformation of cell.

癌变组胸腺细胞中SHP-2 mRNA编码PTPase催化区的700～1096bp间可能出现甲基化；⑦癌变组的胸腺细胞中存在一相对分子质量约为55×10〓的蛋白质，它与IgG具有相同的抗原性，其表达量的变化趋势与SHP-2的蛋白表达变化趋势基本一致，即在胸腺细胞中明显高于对照组及未癌变组，但在骨髓细胞中对照组高于癌变组；⑧SHP-1、SHP-2在γ射线诱发的BEAS-2B细胞恶性转化过程的变化规律：SHP-1蛋白表达量的升高与细胞的恶性转化在时间上基本一致，而SHP-2蛋白表达量与恶性转化无明显关系。

In this study, first time, we investgated the molecular mechanism of pneumcoccal pathogenity, including virulence formation and resistance to β-lactams, on the point view of transformation. 1. In order to offer methods and pneumococci strains for later study, a transformation model of Streptococcus pneumoniae in laboratory was well established, by inducing pneumococci into transformation with CSP.

研究包括以下四部分内容：1、为后继的研究提供方法学支持和所需菌株，本研究利用CSP诱导细菌转化发生，建立了肺炎链球菌的实验室转化模型；并构建了肺炎链球菌转化缺陷菌株，通过抗生素培养基培养和PCR鉴定菌株构建成功。

The comparison and analysis of relevant results.●Application for financial data analysis and prediction. The research work is summarized as follows At first, we have proposed a reformulating and revolutionizing concept for neural networks information processing and transformation mechanism based on the coupling between field organization transformation and field action transformation.

场组织变换通过神经网络的隐单元，把输入模式转换为相关的结构表示，抽取输入模式的主要结构特征，形成外部环境在反应变换中的结构表示；场反应变换把由场组织变换形成的特征表示，转换为实际期望的输出。

Looking to bounce back from defeat against Cesena last weekend, the Calabrian boss will be sticking to his tried and tested 4-3-3, with Lopez and Sedivic joined up front in Giampaolo's absence by either Nanni or Plasmati.

鉴于上周末对切塞纳的比赛中反败为胜，这支南方球队将会更坚持其一贯而有效的4-3-3阵型，洛佩斯和塞德维奇将取代受伤缺席的詹保罗，和纳尼或者帕斯马蒂中的一个搭档锋线。

Meanwhile, their exposure to the more freewheeling ways of the West broadened dramatically.

另外，西方较自由的生活方式对年轻人的影响增大。