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The efficiency of selection was monitored by comparing the number of phage recovered from the acid elution and cell lysate in each round,and by testing EGFR binding specificity of polyclonal phagescFv on CHOEGFRGFP1 and CHOK1 cell with cell ELISA. Bacterial PCR was used to select clones containing a 1 kb insert. Cell ELISA was used to determine EGFR binding specificity of monoclonal pscFv on EGFR positive and negative cell. The number of individual EGFRbinding clones was determined with nucleotide sequencing. Results 500fold enrichments were observed by tittering phages in the cell lysate after five rounds of selection.

以稳定转染的CHOEGFRGFP1细胞和未转染的CHOK1细胞分别作为EGFR阳性和阴性细胞，采用负筛选的方法进行筛选；通过比较每轮投入及洗脱出噬菌体的效价比以及细胞ELISA检测多克隆pscFv与阳性、阴性细胞结合情况对筛选过程进行监测；采用菌落PCR挑选含有全长scFv片断的菌落，进一步用细胞ELISA检测单克隆pscFv与EGFR阳性及阴性细胞结合特异性；挑选EGFR特异性单克隆pscFv采用DNA测序法确定克隆多样性。

To use phage display construction antibody library, trabant peptide library, protein mutant library and cDNA library, thus generally using this technology to screen single chain antibody, le...

利用噬菌体表面展示技术可以构建抗体库、随机肽库、蛋白质突变体库和cDNA文库，从而广泛用于单链抗体的筛选、先导化合物的筛选、疫苗的研制等，尤其是在单链抗体、模拟抗原表位的筛选及建立食品安全快速检测体系方面，具有广阔的应用前景。

The performance of BL3 safety laboratory in preventing experimental aerosol diffusion and sterilizing microbe aerosols through high efficiency filter in a ventilation system was tasted.Aerosols of uranin, vibrio phage and Bacillus subtilis var. niger spores were used for the test.

采用荧光素钠、弧菌噬菌体和枯草芽胞杆菌气溶胶对P3级微生物安全实验室防气溶胶扩散性能及其排风系统中高效过滤器滤除气溶胶的效果进行了检测。

Institute of Microbiology and Epidemicology, Academy of Military medical Science, Beijing,100071,China The performance of BL3 safety laboratory in preventing experimental aerosol diffusion and sterilizing microbe aerosols through high efficiency filter in a ventilation system was tasted.Aerosols of uranin, vibrio phage and Bacillus subtilis var. niger spores were used for the test.

P3级微生物安全实验室防气溶胶扩散性能的检测胡庆轩车凤翔摘要：采用荧光素钠、弧菌噬菌体和枯草芽胞杆菌气溶胶对P3级微生物安全实验室防气溶胶扩散性能及其排风系统中高效过滤器滤除气溶胶的效果进行了检测。

This study was to construct a recombinant T7 phage vaccine expressing xenogenic VEGF on the capsid, and test its inhibitory effect on Lewis lung cancer cells in mice.

本实验采用噬菌体展示技术，构建人血管内皮生长因子重组竹噬菌体疫苗，并检验疫苗对小鼠Lewis肺癌的抑制作用。

It was concluded that a heat-labile serum factor, most likely a type of complement-mediated IgM reaction, is responsible for inactivating phage in the blood.

我们认为，血清中存在的因子是导致噬菌体失活的主要因素，且很可能是由IgM介导的补体引发。

Methods: The human Fd repertories genes were amplified by RT-PCR from PBMC of hepatoma patients, and cloned into the vector pComb3X with chimeric L chain to construct the human-mouse hybrid Fab phage library.

利用RT-PCR自肝癌患者外周血淋巴细胞中扩增全套人抗体重链Fd基因片段，克隆入含嵌合L链的展示载体pComb3X，建立人－鼠杂合Fab噬菌体抗体库。

ABSTRACT In order to screen chicken heart type fatty acid-binding protein gene genomic DNA library was constructed in λ phage.

本研究通过构建北京油鸡基因组DNA噬菌体文库，筛选含鸡心脏型脂肪酸结合蛋白基因的重组子，克隆出4株含鸡H-FABP基因片段的重组噬菌体。

ABSTRACT In order to screen chicken heart type fatty acid-binding protein gene genomic DNA library was constructed in λ phage. Four positive clones containing chicken H-FABP gene were obtained.

本研究通过构建北京油鸡基因组DNA噬菌体文库，筛选含鸡心脏型脂肪酸结合蛋白基因的重组子，克隆出4株含鸡H-FABP基因片段的重组噬菌体。

In this study, we amplified both heavy and light genes from anti-SP hybridoma cells, which were later cloned and expressed on the surface of M13 phage.

在本研究中，我们利用PCR的方式来大量增殖重链和轻链的产物，并将其接到质体上，并表现在的M13噬菌体的表面上。

Putt your way through 36 fun-filled holes of minigolf on 3D designed courses with elevated greens, bunkers, bridges and water hazards, among other crazy obstacles.

您的推杆方式，通过36个有趣的填孔迷你的三维设计的课程，以提升绿党，掩体，桥梁和水的危害，除其他疯狂的障碍。

Some participles can be used either as attributes or as predicatives.

有些分词既可当定语用，也可当表语用。