查询词典 phage

We have first constructed an ouabain ScFv phage library in the world by advanced phage display technique.

应用先进的噬菌体抗体库技术，我们在国际上首次构建了哇巴因噬菌体单链抗体库。

7TM phage display peptide l ibrary was biopanned for 4 rounds to harvest monoclonal anti-body human KGF and then phage tilter was detected.

以人KGF 单克隆抗体为靶，对噬菌体随机七肽库进行4 轮生物淘选，测定噬菌体滴度。

The efficiency of adsorption and removing Poliovirus 1(PV1) and bacteroides fragilis phage by nature and modified sand in the conditions of different environment pH, various mental salts and concentration variation, exist of humic acid in tap and distilled water were studied in this paper.

分别在不同pH、含有不同金属阳离子及腐殖酸的脱氯自来水与蒸馏水中，研究了砂与金属氢氧化物修饰砂吸附与去除Polivirus 1(PV1)和Bacteroides fragilis phage的不同效果。

The Phage display technology is technology that expresses exogenic polypeptide with shell protein of phage by inserting the exogenic DNA into the shell gene.

噬菌体展示技术是一种有效的药物靶点筛选工具，可用其筛选中药的药物靶点蛋白。

VP2 protein of infectious bursal disease virus was displayed on T7 phage surface, and this recombinant phage was then purified and labeled with FITC. The interaction between fluorescigenic phage and IBDV host cells was detected by fluorescent microscope and flow cytometer.

将鸡传染性法氏囊病病毒衣壳蛋白VP2展示到T7噬菌体表面，以FITC标记纯化的重组噬菌体，通过荧光显微镜观察与流式细胞仪检测，研究标记噬菌体与病毒受体细胞——法氏囊B细胞的相互作用。

Among them strain MZ1 had been confirmed a lysogenic strain by indicator strain ZK1 after producing phage plaques induced by Mitomycin C.Two templates of overnight cultivation and its DNA from strain MZ1 were then amplified in the same time with five pairs of primers designed from the same phage,and the same products were obtained,suggesting that strain MZ1 was also confirmed a lysogenic strain by PCR method.

MZ1菌株的过夜培养液与指示菌ZK1(血清型H25)混匀并在双层琼脂上培养12 h后出现了滴度为2×107 pfu的噬菌斑，从而证明生产菌株MZ1确为溶原菌；又以GIL01同源性较高的其它四个基因设计引物，分别以MZ1的培养物及其溶原性噬菌体基因组为模板进行PCR扩增，结果两者得到几乎一致的带型，进一步证明了生产菌株MZ1为溶原菌，说明利用PCR法检测鉴定苏云金杆菌的溶原性是可行的。

Results We obtained a lysogenic bacteriophage MZTP01with clear plaque and 1 mm diameter. Fragment D with 2362bp (Genebank No. AY639599) was obtained after the phage DNA hydrolyzed by HindⅢ/EcoRⅠ. Among fragment D, the pep gene with molecular weight of 47 kDa and length of 1101bp was cloned and expressed. Recombinant M15 (pQE30pep) was built and overexpressed in Escherichia coli with a 47kDa clear band. At the same place a clear band was observed by Western blot. Judging from the time course expression, we could conclude that PEP protein produced at 1 hour after induction and then increased gradually. PEP protein was mainly in the form of inclusion body in the recombinant and slowed the growth speed of host. Homologous comparison of PEP protein from phage MZTP01 with other PEPs from BLAST were that phage MZTP01 PEP protein had 100% homologe with that of Escherichia coli K12, and most of others took the similarity in the range between 37%~84%.

诱导获得的溶原性噬菌体MZTP01斑点清晰，直径约1mm，成斑时间12h；从噬菌体基因组DNA双酶切(HindⅢ/EcoRⅠ)片段中回收长度为2362bp的D片段(Genbank登录号： AY639599)，又从D片段中克隆了长度为1101bp、编码367aa、分子量为47kDa的pep基因，表达载体M15(pQE30pep)在大肠杆菌(Escherichia coli， E.coli)中表达获得了47kDa的清晰表达带，在1h 时开始产生蛋白并有逐步上升的趋势； Western blot 也在47kDa处得到一条清晰的条带；可溶性分析表明PEP蛋白在重组菌株中是以不可溶的包含体形式存在的，该蛋白的产生明显地抑制了宿主的生长速度；噬菌体PEP氨基酸序列之间的同源性比较表明，噬菌体MZTP01 PEP蛋白与来自E.coli K12噬菌体的PEP蛋白的同源性程度最大。

Methods: MUC1/Y extracellular domain was used as a target molecule to biopan Ph. D. 12 phage randem peptide library. Two protocols using affinity gel and cell culture plates respectively were carried out. Positive phage clones were identified by ELISA. ssDNA sequencing was done on 16 positive phage clones to get the amino acid sequences of MUC1/Y-binding peptides. Immunohistochemistry was done to show the capacity and specificity of positive phage clones to bind the tumor cell lines.

以MUC1/Y黏蛋白的胞外段蛋白(MUC1/Yex)为靶分子，用凝胶亲和法和酶联板法分别筛选十二肽噬菌体随机肽库，ELISA鉴定阳性克隆，DNA序列测定后确定MUC1/Yex结合肽的氨基酸序列；免疫组化鉴定阳性噬菌体克隆与正常及肿瘤细胞的结合能力及特异性。

This study is about the production of phage antibody with infection of help phage M13KO7 in a supE suppressor strain (TG1) of E.

本研究是有关噬菌体抗体在大肠杆菌supE抑制性菌株(TG1)中感染辅助噬菌体M13KO7的生产。

In this quiescent state most of the phage genes are not transcribed; the phage genome exists in a repressed state. The phage DNA in this repressed state is called a prophage前噬菌体 because it is not a phage but it has the potential to produce phage.

在静止期内大多数噬菌体基因不能转录，噬菌体基因组处于抑制状态，这样的噬菌体DNA称为前噬菌体，因为此时它并不是一个噬菌体，但它具有产生噬菌体的潜力。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失