查询词典 peroxidase

Objective: To verify whether hot PBS washing after the enzymatic reaction between tyramine and horseradish peroxidase can improve the sensitivity and specificity of catalyzed signal amplification. Methods: Using 7 different types of primary antibodies and 2 tomor microarray system, we carried out EnVision, LSAB, Standard CSA, and modified CSA staining sod compared their sensitivities and specificities. Results: The modified CSA method was the moat sensitive one among the 4 methods.

目的：探讨酪胺与过氧化物酶发生酶促反应后，用热缓冲液洗以进一步提高和改善催化信号放大法的敏感性和特异性方法：应用7种不同类型的一抗，2张不同肿瘤的组织芯片，同时进行EnVision法、LsAB法、标准CSA法和改进CSA法染色，比较敏感性和特异胜。

Patterns of purification of peroxidase having lignin or manganese oxidation activities in white rot fungi have been extensively studied.

关于青霉产生木素过氧化物酶的研究在国外只有零星报道，但尚未有关于其纯化及特性的研究，国内也无此方面的报道。

Using white rot fungi Mn-dependent peroxidase as main enzyme, the nitrobenzene compounds are degraded. And the unique degradation mechanism is studied.

以白腐真菌锰过氧化物酶为主导酶处理硝基苯化合物，并研究了其独特的降解机理。

The double labelling technique by combination of horseradish peroxidaseretrograde tracing with immunohistochemical staining for γ-amino-butyric acidwas used to observe the distribution of GABA-positive neurons in the abducens and vestibular nucleus,and its projection to oculomotor nucleus of rats.

用辣根过氧化物酶(horseradish peroxidase，HRP)逆行标记结合γ-氨基丁酸(γ-amino-butyric acid，GABA)的免疫组织化学双标技术观察大鼠的展神经核和前庭神经核内GABA阳性神经元的分布，以及其向动眼神经核的投射。

Then peroxidase isoenzyme spectrum analysis of five strains of Aleurites fordii.

标题 五个三年桐品种过氧化物酶同功酶的酶谱分析。

Then peroxidase isoenzyme spectrum analysis of five strains of Aleurites fordii.

五个三年桐品种过氧化物酶同功酶的酶谱分析。

Peroxidase settling process was the optimum staining for the fast determination of Alnus formosana pollen viability.

过氧化物酶沉淀法是快速测定台湾桤木花粉生活力的适宜方法。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Some new results are obtained from the studies: 1 a novel thermokinetic research method, the characteristic reduced parameter method, has been proposed, and the kinetics of oxidation of benzoquinone by hydrogen peroxide has also been investigated with this method; 2 the kinetic mathematical models that the metallomicelles made from the mono-nuclear or dinuclear complexes and surfactants catalyze the phenolic derivant oxidation have been established respectively; 3 the feasibility and mechanism that the metallomicelle, a mimetic model of peroxidase, catalyzes the phenolic derivant oxidation has been discussed.

研究得到如下新的结果：(1)提出了一种新的热动力学研究法——特征对比参量法，用该法研究了过氧化氢氧化苯醌反应动力学；(2)分别建立了由单核和双核金属配合物与表面活性剂形成的金属胶束催化酚类氧化反应的动力学数学模型；(3)探讨了金属胶束模拟过氧化物酶催化酚类氧化反应的可行性及其机理。

E. methyl jasmonate +methyl salicylate, MeJA +cis-hexenal, MeJA +trans-hexenal, MeJA +benzothiazole, and then the induced activities of three defensive enzymes including peroxidase, polyphenol oxidase and phenylalanine ammonia lyase were measured.

以1年生合作杨扦插苗为试验材料，利用茉莉酸甲酯分别与水杨酸甲酯、顺式己烯醛(cis-hexenal)、反式己烯醛(trans-hexenal)和苯骈噻唑混合的4种挥发物熏蒸合作杨叶片，检测过氧化物酶、多酚氧化酶及苯丙氨酸解氨酶3种防御酶的活性变化。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。