查询词典 periplasm

Expression level of rhGH could reach 250 mg/L culture solution by means of selecting components of original culture media, components of supplemental media, volume of supplemental media, the time of supplying supplemental media and supplying speed of supplemental media, secretion rhGH was found to represent 44%~54% of the total proteins in periplasm, and the fermentation time was reduced to 8 hrs.

通过对发酵培养基成分、补料成分、补料体积、补料时机和滴加流速的选择，分泌表达rhGH的量可达到250 mg/L，占周质蛋白的44%~54%，发酵周期缩短为8小时。

Coil and B. subtilis using the β-lactamase distribution assay. The results showed that the β-lactamase activities of E.coli are mainly in periplasm while the enzyme produced by B.

内酰胺酶活力测定表明，大肠杆菌产生的酶主要积累在周质空间，而枯草芽孢杆菌产生的酶主要分泌在胞外。

Western blotting confirmed that the low activity of b-lactamase in E. coli Top10 pcs+ resulted from a lower amount of b-lactamase in its periplasm.

由此可见，周质内低含量的b-内酰胺酶是导致E.coli Top10 pcs+细菌氨苄青霉素抗性降低的原因。

In accordance with the invention, the hG-CSF protein can be prepared with high purity through rather simple process facilitating secretion of large amount of hG-CSF fusion protein into the periplasm, which does not require complicated processes such as solubilization and subsequent refolding required for isolation of the hG-CSF protein produced in cytoplasm as insoluble inclusion bodies by conventional techniques, thus, the hG-CSF protein can be widely used as an active ingredient in the development of supplementary agents for anticancer therapy.

根据本发明，通过促进大量hG－CSF融合蛋白分泌到周质中的相当简单的方法可以高纯度制备hG－CSF蛋白，所述方法不需要复杂的加工过程例如对于通过常规技术分离胞质中产生的作为不溶性包函体的hG－CSF所需的溶解和随后再折叠，因此，在开发用于抗癌治疗的补充药物的过程中可用hG－CSF蛋白广泛地作为活性成分。

Results In cystitis glandularis tissue AQP1 mainly expressed in the endothelial cells of micrangium,the immunohistochemical positive exponent was 35.947;AQP2 expressed in the cell membrane and periplasm of the mucosa epithelial cells,the immunohistochemical positive exponent was 34.644;and AQP3 did not express.

结果：在腺性膀胱炎组织中AQP1主要表达在微血管内皮细胞，免疫组化阳性指数35.947；AQP2主要表达在炎性膀胱黏膜上皮细胞的胞膜及胞质内，免疫组化阳性指数34.644；AQP3则呈阴性表达。

Nevertheless, no homologues of typical molecular chaperones have so far been found in periplasm, and the recently reported chaperone activities of periplasmic protein disulfide isomerase and peptidyl pro-lyl isomerase seem to be too weak to satisfy such assumed needs.

虽然膜间质中的蛋白二硫键异构酶和脯氨酸顺反异构酶最近被报导具有分子伴侣活性，但是这种活性的检测使用的不是天然的膜间质蛋白，而且没有捕获到复合物的形成，因此看起来仍不能够满足前面分析的对膜间质蛋白保护的需求。

Expression level of rhGH could reach 250 mg/L culture solution by means of selecting components of original culture media, components of supplemental media, volume of supplemental media, the time of supplying supplemental media and supplying speed of supplemental media, secretion rhGH was found to represent 44%-54% of the total proteins in periplasm, and the fermentation time was reduced to 8 hrs.

通过对发酵培养基成分、补料成分、补料体积、补料时机和滴加流速的选择，分泌表达重组人生长激素的量可达到250mg／L，占周质蛋白的44％～54％，发酵周期缩短为8小时。

For example, it is a pathogenic bacterium and has endotoxins; it secretes protein into the periplasm and often into inclusion bodies. Bacillus subtilis is an attractive host for the production of heterologous secretory proteins. Many genes has succeed expressed in Bacillus subtilis, and the plasmid vectors for Bacillus subtilis are now taken from other genus bacterium, such as Staphylococcus aureus or lactococcus lactis and so on. As it is clear to known that these plasmids are far from optimal for Bacillus subtilis, because of low efficiencies of cloning and high levels of replicating instability.

枯草芽胞杆菌是目前原核表达系统中分泌表达外源蛋白较理想的宿主，近几年已在枯草芽胞杆菌中成功表达了多种基因，但大多都应用来源于金黄色葡萄球菌、乳酸杆菌等的载体来表达外源基因，由于其在枯草芽胞杆菌中存在分离复制的不稳定及外源基因的克隆表达效率低的现象，故都不是枯草芽胞杆菌的最佳选择，还有待改进。

The most of the product became soluble from inclusion body by lowering induction temperature. At the same time the gene was cloned into vector pMAL-p2X, which was translated in E. coli TB1 and expressed a fusion protein. We optimized the induction time and achieved high expression. But there was no obvious product secreted in the periplasm extract. Almost all fusion protein is expressed soluble in cytoplasm.

利用融合型表达载体pTYB11-EFE融合型表达，对诱导剂IPTG的浓度和诱导温度进行了优化，通过降低诱导温度使产物由包含体变成可溶性融合型产物；利用融合型分泌表达载体pMAL-p2X构建了表达载体pMAL-p2X-EFE-7~#，对诱导时间进行了优化，获得了较高的表达量，但发现产物没有明显的分泌表达，绝大部分是以可溶形式存在于胞内。

It shows that coexpression of secE/Y gene can increase a new band of about 25kD in the periplasm, but further electrophoresis analysis and Western blot prove that this band may not be the assembled TGF β1 dimer.

发现共表达secE／Y基因可使周质中新增25KD的蛋白带。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。