查询词典 periplasm

ELISA and MTT assay show that coexpression of 〓 gene and dsbA gene, can increase the total content of TGF β1 in the periplasm, and gain biological activities.

而ELISA测定和MTT测活都表明，共表达〓和dsbA基因，使得周质中TGFβ1的含量明显增高，并具有显著的生物学活性。

The results showed that the three scFvs specific for Xac were successfully expressed in E. coli HB2151. The molecular weight of expressed products was about 32.0×103 and the products were mainly located in the periplasm.

结果显示，3株抗柑桔溃疡病菌可溶性单链抗体在大肠杆菌HB2151中均获得成功表达，表达产物分子量约为32.0×10^3，主要集中于细菌周质腔中。

Carrying out the work in a blue-green algae, a cyanobacterium, the team has been able to show that a protein requiring copper transports to the periplasm, the outer area of the cell, where it then folds around the available metal, which is copper.

研究组已能了解到，蓝绿藻在执行生理功能的过程中，铜蛋白转移到细胞外的细胞周质，在那里对可用的金属铜进行包绕。

Methods Ampicillin inhibitionof E. coli Top10 pcs+ was tested at first, and then b-lactamase activity in periplasm was examined.

然后再使用抗生素抗性分析、b-内酰胺酶的酶活测定以及Western blot杂交技术，分析质粒编码的b-内酰胺酶从细胞质到细胞间质的分泌情况。

Finally Western blot was used to detect the amount of b-lactamase in both bacterial periplasm and cytoplasm. Antibiotic tests showed that high concentrations of ampicillin inhibited the growth of E.

抗生素抗性分析发现，高浓度的氨苄青霉素抑制E.coli Top10 pcs+细菌的生长的氨苄青霉素剂量低于对照组，其半致死剂量IC50在700~800mg/mL之间。

In normal bladder tissue, AQP1 mainly expressed in the endothelial cells of capillary and arteriolar under the mucous membrane of urinary bladder,AQP2 and AQP3 expressed in the celluar membrane and periplasm of the mucous membrane of urinary bladder.

AQP1在正常膀胱组织中主要表达于膀胱黏膜下微血管和小动脉的内皮细胞， AQP2和AQP3表达于膀胱黏膜上皮细胞的细胞膜及胞质中。

With the inducing time prolonged, the amount of fusion protein increased, and the highest content amounted to 39. 14% of total protein. Different temperature (15℃, 25℃ and 37℃) could induce expression of fusion protein, and the higher inducing temperature was, the more total fusion protein and soluble fusion protein was produced. Most fusion protein was expressed in the cytoplasm and was soluble, a little of fusion protein was expressed in periplasm or expressed as inclusion bodies, and no fusion protein was found in culture. Furthermore, the results of in vitro enzymatic reaction suggested that the fusion protein could catalyze the methylation of theobromine.

随着诱导时间的延长，表达的融合蛋白产量逐渐增加，表达蛋白总量最高可达到菌体总蛋白的39.14％；在15℃、25℃和37℃三个不同的诱导温度下，均能诱导产生融合蛋白，而且产生的融合蛋白的总量及其可溶性部分所占比例均随诱导温度升高而增加；在菌体的各个部位中，融合蛋白主要在细胞质中以可溶的形式进行表达，有少量在外周质中表达或以包含体形式在细胞质中表达。

The results showed the bacterial supernatant and periplasm containing NKAT2 scFv were able to bind the NKAT2 receptors expressed on cell surface,but NKAT4 scFv failed to bind to NKAT4 receptors.

结果显示，含NKAT2－scFv的细菌上清及胞质提取物均可识别到表达在细胞表面的NKAT2受体分子，但含NKAT4－scFv的细菌上清及胞质提取物均未成功

After infecting E.coli HB2151 cells with one positive clone,soluble recombinant antibodies about 27 kDa were produced and located in the periplasm and the supernatant.

约27kDa大小的ER以可溶形式存在于细胞质及细胞周质中，并可分泌至上清。

Coli. Transmission electron microscopy was used to evaluate the cell morphology of both the normal and the treated E. coli. The observation with TEM suggested that silver nanoparticles lead to the formation of "pits" in cell wall of the bacteria, and silver nanoparticles could enter into periplasm through the pits and destroyed the cell membrane.

采用透射电镜观察了经纳米银粒子处理过的大肠杆菌细胞形态变化过程，结果显示纳米银粒子先在细胞壁上产生小的孔洞，通过这些孔洞进入周质空间，导致细胞膜成分渗漏和破坏细胞膜，进而进入细胞内部。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。