查询词典 peripheral

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Peripheral neutrophils are activated in anaphylactoid purpura, which seems to be more obvious in active phase than in remission phase.

过敏性紫癜患者外周血中性粒细胞处于激活状态，活动期较缓解期更明显。

The tissue samples of small intestine, liver and blood samples from peripheral and portal vein of 4 macaques of 6-month fetus, 2-day neonate, 45-day neonate and adult were obtained after anesthetization.

通过手术途径获得胚胎6月、新生2 d、新生45 d和成年猕猴的回肠、肝脏、门静脉和外周血等标本，应用放射免疫分析法测定各标本中的VIP含量；通过免疫组化方法观察VIP在肠、肝组织内的分布；利用原位杂交法检测VIP受体1（VIP receptor 1，VIPR1）的表达。

Anisotropically etched insulative sidewall spacers are formed over opposing sidewalls of individual of said transistor gate lines within the peripheral circuitry area prior to forming anisotropically etched insulative sidewall spacers over opposing sidewalls of individual of said transistor gate lines within the memory array area.

在所述存储器阵列区域内的所述晶体管栅极线中的个别者的相对侧壁上形成各向异性蚀刻的绝缘侧壁隔离物(40)之前，在所述外围电路区域内的所述晶体管栅极线中的个别者的相对侧壁上形成各向异性蚀刻的绝缘侧壁隔离物(34)。

Chronic benzene poisoning can cause DNA impairment of marrow cells and peripheral lymphocytes and reduce the activity of antioxidase.

慢性苯染毒可致小鼠骨髓细胞及外周血淋巴细胞 DNA损伤，体内抗氧化酶活性降低。

The peripheral venous blood was sampled before operation and on the postoperative days 1 and 7 to determine the serum levels of IgG, IgA, IgM, the percentage of T lymph cell subgroups CD3, CD4, CD4/CD8 and CRP, PGE,α- antitrypsin etc.

分别于术前、术后第3 天及术后第7 天采集外周血标本，检测免疫功能指标IgA、IgG 和IgM，采用免疫组织化学法测定T 淋巴细胞亚群及炎症指标。

Objective In order to know the essential meaning of immune disorder in Recurrent Aphthous Ulcer patients and to search the agreeable treatment meanwhile, the cluster of differentiation of peripheral lymph cells were detected and analyzed by flow cytometry, and afterwards those patients were treated by immunomodulator.

目的为了更好的理解RAU患者免疫紊乱的实质及寻找有效的治疗方法，我们采用流式细胞技术，对RAU患者发病期和康复期外周血淋巴细胞的表型进行测定、分析，探索流式细胞技术在RAU的诊断及指导临床治疗方面的作用。

MATERIALS:Peripheral blood was procured from 29 patients with severe and chronic aplastic anemia.

材料：来源于29例重型及慢性再生障碍性贫血患者的外周血。

MethodsThe immunosuppressive mouse model was established by using hydrocortisone. Huangqi Fuzhengtang and Yupingfeng San apozema were given to the mice intragastrically. The contents of hemolysin, IL-2, and INF-γ in the mouse serum and the expression of CD3+, CD4+, and CD8+ in the peripheral blood lymphocytes were measured.

以氢化可的松造成免疫低下小鼠模型，用黄芪扶正汤和玉屏风散水煮液进行灌胃，检测小鼠血清溶血素的生成、IL-2和INF-γ的含量以及外周血淋巴细胞CD3+，CD4+和CD8+的表达。

Methods: To detect the expression of c-myc mRNA and TA in tumor tissues ,the approximal and distal peripheral tissues, every lcm away from the tumor margin of 12 gastric and 18 colorectal carcinomas with RT-PCR and TRAP methods respectively.

用RT-PCR和TRAP方法检测12例胃癌和18例肠癌标本和切缘组织中c-myc基因mRNA和端粒酶活性的表达。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。