查询词典 performed

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象，提取细菌基因组DNA，用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切，回收大小为1～5Kb的DNA片段，将其连接入原核表达载体pET-28a-c，最后转化宿主菌，结果成功地构建了基因表达文库，为后续的基因筛选工作奠定基础；另外，本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计，以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板，进行PCR扩增反应，结果表明成功地获得两个新基因：23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸)，并进一步做了不同物种之间的分子系统发育分析，构建了基于23S rRNA基因的邻接法系统发育树，阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

These functions include cooperative medical insurance for rural and urban residents originally performed by Jiaxing Municipal Bureau of Health, the organizing function of fund for rural and urban minimum living and medical aid performed by Civil Affairs Bureau, the organizing and issuing of the fund for rewarding rural households who carry out the one-child policy performed by Municipal Committee of Population and Family Planning, and the unemployment insurance undertaken by Employment Service Bureau.

据了解，新成立的市社会保障事务局将以市社会保险事业局为基础，整合市卫生局承担的城乡居民合作医疗保险职能，市民政局承担的相关精简职工遗属服务和城乡居民最低生活保障金、医疗救助金的组织职能，市人口计生委承担的有关农村部分计生户奖励扶助金的组织发放职能，市就业管理服务局承担的职工失业保险等职能。

Methods:clinical data of 23 cases of left-sided colonic carcinoma undergoing one-stage resection and primary anastomosis were analyzed respectively.intraoperative colonic lavage were performed in all cases.for the 14 cases which were considered to be dangerous,preventive ceostomy was performed.results:23 cases were performed one-stage resection and primary anastomosis,all patients had not any complications such as anastomatic leakage and infection of the peritoneal cavity.conclusions:have a strict control over operation indication,perform postoperative care properly, one-stage resection and primary anastomosis for acute obstruction of left hemicolonic carcinoma is safe and workable.

回顾性分析我院五年来23例左半结肠癌并急性梗阻一期切除吻合术的临床资料，所有病例均术中结肠灌洗，而认为有危险性的14例同时行预防性盲肠造瘘。结果：23例一期切除吻合术，无一例发生吻合口漏、腹腔感染等并发症。结论：严格掌握手术适应症，积极术前准备，充分结肠灌洗，对认为有危险性吻合口实行预防性盲肠造瘘，正确术后处理，左半结肠癌并急性梗阻一期切除吻合术是安全可行的。

A simple tenotomy was performed in thirty-nine cases, and a tenodesis was performed in thirty-three. No associated acromioplasty was performed.

其中39例患者行简单的肌腱切除术；33例行肌腱固定术，未行相关的肩峰成形术。

The study was performed as follows:1culture of pathogenic fungi and fungus identification in 110 cases.2antifungal susceptibility test with Etest strip by ketoconazole,itraconazole,Fluconazole,Fluorocytosine,and amphotericinumB.3Topical application of doktarin ps cream,ketoconazole cream,clotrimazole ung,and thymol alcohol were performed in 4 groups and the intermittent impulsive treatment in who failed to respond to local treatment were performed too.

①从325例本病患者中随机选出的110例进行真菌培养及菌种鉴定。②用Etest技术，以酮康唑、伊曲康唑、氟康唑、氟胞嘧啶及二性霉素B5种药物试条进行药敏测试。③将325例患者随机分4组，分别用复方达克宁霜、酮康唑霜、克霉唑软膏及麝香草酚酒精作局部治疗；对局部治疗无效的30例，用IT作间歇冲击治疗。

Following studies had been performed:(1)repetitive WLT and real-time ultrasonography had been performed to 18 HS and 40 FD toassess the reproducibility and influencing factors.(2) correlations of WLT with symptomscore and electrogastrography had been analysed.(3) differences ofWLT between HS and FD had been compared.(4) methophenolane andimmunohistochemistry stain of gastric fundus mucosa biopsy from 40 FD patients hadbeen performed to analyse the correlations of symptom score and WLT with count andactivity of mast cells, 5-HT level, and H.Pylori infectious status.(5) the effect of oralsumatriptan to proximal stomach function in 10 HS and 10 FD had been assessed, so as toestablish the possible regulating mechanism of fundic relaxation, explore the therapeuticpotential and feasibility of sumatriptan on FD.Results:I. Evaluation of WLT and its application in FD management.

我们主要进行以下几个方面的研究：(1)对18名健康人及40名FD患者重复进行水负荷试验并结合B超声对近端胃的实时监测，评价水负荷试验结果的可靠性并分析其影响因素；(2)观察水负荷试验结果与症状积分及体表胃电结果的相关性；(3)比较FD患者和健康成人水负荷试验结果的差别；(4)对40名FD患者胃底粘膜活检组织进行免疫组化染色和甲苯胺蓝染色，观察FD患者的症状及水负荷试验结果与胃底粘膜肥大细胞、5-HT表达情况及H.pylori感染情况的相关性：(5)观察口服舒马曲坦对10名健康人及10名FD患者近端胃功能的影响，并由此推测胃底舒张运动调节的生理机制，探讨口服舒马曲坦治疗FD的可行性。

Drug sensitive test and three-dimensional test220 strains of Pa were isolated from hospitalized patients between 2003 and 2007. K-B method was used to tested the susceptibility of 10 different antibiotics. IRPa was screened by testing the minimal inhibitory concentration of imipemem by using agar diluiion method.The susceptibility of these IRPa to the antibiotics was analysised. Three-dimensional test was used to identify the different kinds of beta lactamases from 220 strains of Pa.2.Carbarpenems hydrolytic enzyme genes and oprD2 gene were detectedamong the selected IRPa strains, PCR method was performed to detect carbapenemase genes which included GES、KPC、SPM、VIM、IMP、GIM gene and the oprD2 gene;Multiplex PCR were used to detect OXA genes and plasmid-mediated AmpC beta lactamase genes; The expression of the chromosomal AmpC beta lactamases and oprD2 genes in IRPa strains were analyzed by Real-time PCR.3.Identification and characterization of integronsIntegrase gene was detected by PCR, and the classification of integrons was performed by using restriction fragment length polymorphism.PCR was performed to detect the qacE△1-sull gene,and the gene cassetes which are located at variable region of integrons in the strains were detected to be positive.

方法1、药敏实验和三维实验收集2003～2007年临床分离的220株Pa，对这些菌株采用K-B法测定10种临床常用抗生素的药敏情况，同时采用琼脂稀释法检测亚胺培南的最低抑菌浓度(Minimal inhibitory concentration，MIC)，筛选出对亚胺培南耐药的铜绿假单胞菌，并分析其对其它抗生素的药物敏感率；采用三维实验的方法分析220株Pa产β内酰胺酶的类型。2、碳青霉烯类水解酶和oprD2蛋白的检测针对鉴定的IRPa菌株，采用普通PCR方法检测具有碳青霉烯水解作用的β内酰胺酶耐药基因(GES、KPC、SPM、VIM、IMP、GIM基因)和oprD2基因，采用多重PCR的方法检测OXA型基因和质粒携带的AmpC酶基因，用荧光定量RT-PCR方法检测oprD2蛋白基因表达情况；同时对产AmpC酶的Pa(25株，含IMP耐药和敏感株)用RT-PCR方法检测AmpC酶基因的表达量情况。3。

Three Cases with hydronephrosis were located in left kindey, and 3 cases in bilateral kidneys. Of 5 kidneys in 4 cases of ureteropelvic junction obstruction, Anderson-Heny's procedure was performed in 4 kidneys, and nephrectomy was performed in 1 hydronephrotic kidney because the renal function had lost. Cohen's procedure was performed in all 3 individual hydronephrotic kidneys in 2 cases.

病因及治疗：肾盂输尿管连接部梗阻4例5只肾，4只肾做了Anderson-Heny's手术，1只肾无功能行肾切除术；2例3只肾为巨输尿管症，均行Cohen's法输尿管膀胱移植术，其中1例合并膀胱重复畸形，手术中同时行共同壁切除；l例出生后肾积水渐减轻，未特殊处理，正在随访中。

Methods DSA was performed in 56 patients with intercranial artery TIA and the clinical and distribution of carotid artery stenosis were analyzed, while TCD was performed in 46 patients and CTA was performed in 54 patients with TIA, then sensitivity, specifity and total consistent rate of TCD and CTA were calculated with Kappa test.

选取56例颈内动脉TIA患者行全脑血管造影检查，结合DSA检查结果分析其临床及颈动脉狭窄特点；同时对46例患者进行了TCD检查，54例患者进行了CTA检查，应用Kappa检验分析TCD和CTA检查的敏感性、特异性和符合率。

There were 27 cases of indirect inguinal hernia and 4 cases of direct hernia and 1 case of compound hernia.Transabdominal preperitoneal prosthetic was performed in 19 cases,intraperitoneal onlay mesh was performed in 12 cases,total extraperitoneal prosthesicwas performed in 1 case.

行腹膜前补片修补术(transabdominal preperitoneal prosthesis，TAPP)19例，行腹腔内补片植入术(intraperitoneal onlay mesh，IPOM)12例，行完全腹膜外补片植入术(total extraperirtoneal prosthesis，TEP)1例。

Hanna: That's over now, isn't it?

都结束了，对吗

You must be ill. You look so pale.

你一定是病了，你的脸色苍白。