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The effects and mechanism of GABAergic neurons, NOergic neurons, opioid peptide and cyclic adenosine monophosphate in the nucleus reticularis thalami on sleep-wakefulness cycle of rats and the effects and mechanism of the 5-HTergic nerve fibers project from the nucleus raphes dorsalis to RT on sleep-wakefulness cycle of rats were investigated with the methods of brain stereotaxic, nucleus spile, microinjection and polysomngraphy.1. The effects of GABAergic neurons in RT on sleep-wakefulness cycle of rats1.1 Microinjection of 3-mercaptopropionic acid (3-MP, a kind of glutamate decarboxylase inhibitor) into RT. On the day of microinjection, sleep only decreased a litter. On the second day, sleep marked decreased and wakefulness marked increased. On the third and fourth day, sleep and wakefulness stages resumed to normal.1.2 Microinjection of gamma-amino butyric acid (GABA 1.0μg) into RT enhanced sleep and reduced wakefulness compared with control; while microinjection of L-glutamate (L-Glu, 0.2μg) decreased sleep and increased wakefulness; microinjection of bicuculline (BIC, 1.0μg), a GABAA receptor antagonist, enhanced wakefulness and reduced sleep; microinjection of baclofen (BAC, 1.0μg), GABAB receptor agonist, had the same effects as GABA.2. The effects of NOergic neurons in RT on sleep-wakefulness cycle of rats2.1 Microinjection of L-arginine (L-Arg, 0.5μg) into RT decreased sleep compared with control, but there were on statistaical difference between L-Arg group and control; while microinjection of sodium nitroprusside (SNP, 0.2μg), a NO donor into RT, sleep marked decreased and wakefulness marked increased. Microinjection of nitric oxide synthase inhibitor, N-nitro-L-arginine (L-NNA, 2.0μg) into RT enhanced sleep and reduced wakefulness.2.2 After simultaneous microinjection of L-NNA (2.0μg) and SNP (0.2μg) into RT, SNP abolished the sleep-promoting effect of L-NNA compared with L-NNA group; after simultaneous microinjection of L-NNA (2.0μg) and L-Arg(0.5μg) into RT, we found that L-NNA could not blocked the wakefulness-promoting effect of L-Arg.3. The effects of opioid peptide in RT on sleep-wakefulness cycle of rats3.1 Microinjection of morphine sulfate (MOR, 1.0μg) into RT increased wakefulness and decreased sleep compared with control; while microinjection of naloxone hydrochloride (NAL, 1.0μg), the antagonist of opiate receptors, into RT, enhanced sleep and reduced wakefulness.3.2 After simultaneous microinjection of MOR (1.0μg) and NAL (1.0μg) into RT, the wakefulness-promoting effect of MOR and the sleep-promoting effect of NAL were not observed compared with control.4. The effects of cAMP in RT on sleep-wakefulness cycle of rats Microinjection of cAMP (1.0μg) into RT increased sleep and decreased wakefulness compared with control; microinjection of methylene blue (MB,1.0μg) into RT enhanced sleep and reduced wakefulness compared with control.5. The effects of the 5-HTergic nerve fibers project from DRN to RT on sleep-wakefulness cycle of rats5.1 When L-Glu (0.2μg) was microinjected into DRN and normal sodium (NS,1.0μg) was microinjected into bilateral RT. We found that sleep was decreased and wakefulness was increased compared with control; when L-Glu (0.2μg) was microinjected into DRN and methysergide (MS,1.0μg), a non-selective 5-HT antagonist, was microinjected into bilateral RT, We found that sleep was enhanced and wakefulness was reduced compared with L-Glu group.5.2 When p-chlorophenylalanine (PCPA, 10μg) was microinjected into DRN and NS (1.0μg) was microinjected into bilateral RT, We found that sleep was increased and wakefulness was decreased compared with control; microinjection of 5-hydroxytryptaphan (5-HTP, 1.0μg), which can convert to 5-HT by the enzyme tryptophane hydroxylase and enhance 5-HT into bilateral RT, could block the effect of microinjection of PCPA into DRN on sleep-wakefulness cycle.

本研究采用脑立体定位、核团插管、微量注射、多导睡眠描记等方法，研究丘脑网状核(nucleus reticularis thalami，RT)中γ-氨基丁酸(gamma-amino butyric acid ，GABA)能神经元、一氧化氮(nitrogen monoxidum，NO)能神经元、阿片肽类神经递质、环一磷酸腺苷(cyclic adenosine monophosphate，cAMP)及中缝背核(nucleus raphes dorsalis，DRN)至RT的5-羟色胺(5-hydroxytryptamine，5-HT)能神经纤维投射对大鼠睡眠-觉醒周期的影响及其作用机制。1 RT内GABA能神经元对大鼠睡眠-觉醒周期的影响1.1大鼠RT内微量注射GABA合成关键酶抑制剂3-巯基丙酸(3-MP，5μg)，注射当天睡眠时间略有减少，第二日睡眠时间显著减少，觉醒时间明显增多，第三、四日睡眠和觉醒时间逐渐恢复至正常。1.2大鼠RT内微量注射GABA受体激动剂GABA( 1.0μg)后，与生理盐水组比较，睡眠时间增加，觉醒时间减少；而RT内微量注射L-谷氨酸(glutamic acid， L-Glu， 0.2μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAA受体阻断剂荷包牡丹碱(bicuculline，BIC，1.0μg)后，睡眠时间减少，觉醒时间增加；RT内微量注射GABAB受体激动剂氯苯氨丁酸(baclofen，BAC，1.0μg)后，产生了与GABA相似的促睡眠效果。2 RT内NO能神经元对大鼠睡眠-觉醒周期的影响2.1大鼠RT内微量注射NO的前体L-精氨酸(L-Arg，0.5μg)后，与生理盐水组对比，睡眠时间略有减少，但无显著性意义；而RT内微量注射NO的供体硝普钠(Sodium Nitroprusside，SNP，0.2μg)后可明显增加觉醒时间，缩短睡眠时间；微量注射一氧化氮合酶抑制剂L-硝基精氨酸(L-arginine，L-NNA，2.0μg)后，引起睡眠时间增多，觉醒时间减少。2.2大鼠RT内同时微量注射L-NNA(2.0μg)和SNP(0.2μg)后与L-NNA组比较发现SNP逆转了L-NNA的促睡眠作用；RT内同时微量注射L-NNA(2.0μg)和L-Arg(0.5μg)后，与L-NNA(2.0μg)组比较发现L-Arg可以增加觉醒而缩短睡眠，其促觉醒作用未能被NOS的抑制剂L-NNA所逆转。3 RT内阿片肽对大鼠睡眠-觉醒周期的影响3.1大鼠RT内微量注射硫酸吗啡(morphine sulfate，MOR，1.0μg)后与生理盐水组对比，睡眠时间减少而觉醒时间增加； RT内微量注射阿片肽受体拮抗剂盐酸纳洛酮(naloxone hydrochloride，NAL，1.0μg)后与生理盐水组比较，睡眠时间增加而觉醒时间减少。3.2大鼠RT内同时微量注射MOR(1.0μg)和NAL(1.0μg)后，与生理盐水组对比，原有的MOR促觉醒效果和NAL的促睡眠效果都没有表现。4 RT内环一磷酸腺苷信使对大鼠睡眠-觉醒周期的影响大鼠RT内微量注射cAMP(1.0μg)后与NS(1.0μg)组比较，睡眠时间增多而觉醒时间减少；RT内微量注射亚甲蓝(methylene blue，MB，1.0μg)后，与NS组比较，睡眠时间增多而觉醒时间减少。5中缝背核投射到丘脑网状核的5-羟色胺能神经纤维对大鼠睡眠-觉醒周期的影响5.1大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 0.2μg)比较，睡眠时间减少，觉醒时间增多；大鼠DRN内微量注射L-Glu(0.2μg)，同时在双侧RT内微量注射二甲基麦角新碱(methysergide， MS， 1.0μg )后，与对照组(DRN注射L-Glu 0.2μg，双侧RT注射NS 1.0μg)比较，睡眠时间增多，觉醒时间减少。5.2大鼠DRN内微量注射对氯苯丙氨酸(p-chlorophenylalanine，PCPA，10μg)，同时在双侧RT内微量注射NS (1.0μg)后，与对照组(DRN和双侧RT注射NS， 1.0μg)比较，睡眠时间增多，觉醒时间减少；大鼠DRN内微量注射PCPA(10μg)，产生睡眠增多效应后，在双侧RT内微量注射5-羟色胺酸(5-hydroxytryptaphan ， 5-HTP， 1.0μg )后，与对照组(DRN注射PCPA 10μg，双侧RT注射NS 1.0μg)比较，睡眠时间减少，觉醒时间增多。

No matter B2B, B2C or C2C, penetrate the service condition which Internet the timeliness and 24 hours whole year not rests, may let the enterprise the business hour no longer receive limits, more further is the network does not have the national boundary, each consumer no longer subordinates a Yu area or the country, so long as the consumer links on Internet to be allowed from local to have to the world erects the goods which the electronic commerce website the enterprise purchases needs, moreover business transaction also may on-line penetrate the website directly in to complete, not only may greatly reduce the enterprise cost, also may let the consumer have the more time long travel to window-shop to the network, Enjoys the shopping the pleasure to utilize the ASP technology and the network database principle, I has developed an on-line shopping system based on the B/S pattern.

不管B2B、B2C或C2C，透过Internet的实时性及24小时全年无休的营运状态，可以让企业的营业时间不再受限，更进一步的是网络无国界，每一个消费者已不再隶属于某一地区或国家，只要消费者连上Internet就可以从本地向全世界有架设电子商务网站的企业购买所需的物品，而且买卖交易也可以透过网站间接在在线完成，不但可以大大降低企业本钱，也可以让消费者有更多的时间悠游于网络逛街，享受购物的乐趣。运用ASP技术和网络数据库原理，基于B/S模式我开发了一个网上购物系统。

The skill sets needed to penetrate a network for intelligence-gathering purposes in peacetime are the same skills necessary to penetrate that network for offensive action during wartime, notes a recent congressional report on China's alleged clandestine cyberattacks in the U.S. According to the report, released in October by the congressionally mandated U.S.

和平时期这些手段能够用来侵入网络，收集情报，在战争时期就可以用同样的手段侵入网络，做出攻击，一份最近的美国国会报告中如是写道，这份报告就是关于中国对美国实施的所谓秘密网络攻击。

C. Electro-osmosis technology: the role of electroosmosis in the main material to be dispersed elements to help them successfully penetrate into the skin and internal, the nature of the charge the same mutually exclusive, so the nutritional elements of the cathode current is imposed, can be nutritional elements into the skin tissue .

C。电渗透技术：电渗主要作用于将要分散的物质分子，帮助其顺利穿透并进入肌肤内部，性质相同的电荷相互排斥，因此对正极的营养分子施加正电流，可将营养分子推入皮肤组织。

C. electro-osmosis technology: the role of electroosmosis in the main material to be dispersed elements to help them successfully penetrate into the skin and internal, the nature of the charge the same mutually exclusive, so the nutritional elements of the cathode current is imposed, can be nutritional elements into the skin.

C。电渗透技术：电渗主要作用于将要分散的物质分子，帮助其顺利穿透并进入肌肤内部，性质相同的电荷相互排斥，因此对正极的营养分子施加正电流，可将营养分子推入皮肤组织。

Silk is woven as the raw material into white greige, which is dip-dyed with juice of Shoulang Yam (a herbal medicine exclusively produced in Guangdong). The dip-dyed white greige is spread on lawn before sunrise. The cloth is daubed with unpolluted mud of small pond, so that the juice of Shoulang Yam and the mud will penetrate into each other under the evaporating effect of dew. This procedure must be finished before day break.

它是以桑蚕丝为原料织成白坯绸，再用广东地区特有的植物中草药——薯莨的汁液浸染，在日出前将经过浸染的白坯绸铺在草地上，布面涂抹无污染的小河塘泥，使薯莨汁与塘泥在露水的蒸腾中相互渗透交融，这道工序必须要在天亮前完成。

The development of digital technology influences on the music industry radically on the internet time. The Phonogram and Videogram Producers have to transfer into digital modality. New competition elements penetrate, such as Online-music Operator, the Service Provider, and the Terminal Facilities Producer, go into the industry . Even the Mobile Operator would like to lead the development of the music industry by the music digital operation. There are gambling and amalgamation among groups for the increasing future of digital music.

网络时代数字技术的发展对音乐产业产生了颠覆性的影响，传统唱片公司不得不进行数字化转型，产业链加入了新的竞争力量——网络音乐运营商、增值服务提供商、终端设备制造商，甚至移动运营商以其音乐数据业务欲主导音乐产业的发展，各种力量之间的博弈、融合均为了未来数字音乐极具潜力的发展前景。

That word has a very deep meaning, deliberate, which means weigh together, take counsel together, nourish each other, not physically, I hope, but psychologically, intellectually and penetrate as deeply as possible into one's own consciousness, into its own function, its own way of thinking, living.

这个词的含义很深，商讨，意味着我们一起考虑，一起协商，互相滋养，我希望，不是从身体上，而是从心理上，从智识上，尽可能地深入每个人自己的意识深处，深入它自己的运作过程，它自己的思维和生活方式。

The phase between 1086 and 1093, when he was in Kai Feng, the Capital of Song during the Yuan You times, he continued to penetrate into Buddhism by assimilating the Zen thought "Easy mind leads to the right way" and harmonizing his belief into daily life, so as to fuse the thoughts of Buddhism and Confucianism and form his philosophy of "beyond the earthliness without escape" and the character of "Mask my brightness with the dust of the Earth, whenas conceal clear differentiation in mind."

元丰七年，作《发愿文》，标志着他正式成为一名佛教徒，把佛教作为自己的终身信仰；元佑京师期间(1086-1093)是他学佛的深入期，他吸收禅宗"平常心是道"的思想，把信仰融入生活，将儒、佛思想紧密结合，形成了"超世而不避世"的人生态度与"俗里光尘合，胸中泾渭分"的性格；贬谪黔宜期间(1094-1105)，是黄庭坚参禅的突破期，他勘破心。。。

In the selection of the program made full use of the original base of the water, excavating the stream, waterfall or arranged to create a style unique natural water features, cleverly water park landscape will be extended to the sea, the vastness of the sea will penetrate into the park landscape, Park and will be classified as "Lingbo Rainbow","Water Italy Rong","Bihai grievances","Lin Feng-tao" four scenic areas, ready to develop into the city after万绿园Following the second large public green space.

在遴选程序充分利用了原有的基础上的水，开挖流，瀑布或安排建立一个风格独特的自然水景，巧妙地用水将公园景观延伸到大海，辽阔的大海将渗透到公园景观，公园，将被归类为&凌波彩虹&，&水意大利荣&，&碧海冤情&，&林风涛&四个景区，准备发展成为该市继后万绿园第二个大型公共绿地。

As of Tuesday, Google's results were still censored in China.

截至周二，谷歌的搜索结果仍受中国审查。

In order to make the positive action increase and negative one decrease, the sub-forces of the social factors must be adjusted to form a centripetal force.

在这一过程中，人的主体性发挥是社会有机体健康发展的灵魂。