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Because of inappropriate operation between oncoming straight and left-turn vehicles of signalized intersections of urban areas in Taiwan the department of traffic ever offered corrigible opinions involve in right of way, center of intersections adjudging hard and indirectly encouraged to accelerate left-turn vehicles. But all still lack quantification analysis. Therefore, this problem is worth studying.This study will aim at safe passage of oncoming straight and left-turn vehicles for at-grade signalized intersections. So, this study will be based on row's theory, theory of traffic conflicts, and take many factors into consideration, such as the width of the intersections, types of the intersections, center of the intersections, the size of the vehicles, the location of the vehicles, the radius of turn, paths of operation, the speed of the vehicles, the types of collisions, the angle of road, and also deduce formulas of coordinate of paths. The main method used in this research is the simulation of different situations. By the method, the analytic model of safe passage of oncoming straight and left-turn vehicles for at-grade signalized intersections will be established, and this study will also subsume critical speed of conflict and expected number of times of safe passage. Finally involved rules of safe passage will be proffered.

鉴於台湾都市地区路口对向直行左转车辆常遭受对向车辆不当驾驶行为干扰，因而道路交通主管机关曾针对路口对向直行左转相关道路交通法规可能产生之对向直行左转路权判断不易、路口中心处不易区分、间接鼓励路口转弯车加速左转等问题提出检讨之修正意见，唯整体考量仍缺少〝量化〞分析，系一值得研究之课题，因此，本研究将针对号志化平面路口对向直行左转车辆安全通行问题於法规层面上之特殊性，以交通冲突理论、路权理论为基础，并考量路口宽度、路口型式、路口中心处、车辆尺寸、转弯半径、运行轨迹、行驶速率、行车位置、最终碰撞型态、道路交角等因素，先行推导车辆行驶轨迹座标公式，再利用情境模拟分析法，进行号志化平面路口对向直行左转车辆安全通行分析，分析过程中则纳入冲突临界速率与安全通行期望次数理念，并根据路口对向左转车辆安全通行轨迹、速率集中分布范围之分析结果及相关理论，研拟号志化路口对向左转车辆安全通行轨迹、速率，及对向直行左转车辆路权判断原则，以供驾驶者路口直行左转安全通行之参考。

The cells were cultured under the following serum microenvironment. The primary cells of autoserum group were cultured with autoserum, changing the medium with fetal bovine serum after passage. The primary cells of homogeneity foreign serum group were cultured with homogeneity foreign serum, changing the medium with fetal bovine serum after passage. The primary cells of fetal bovine serum group were cultured with fetal bovine serum, and cultured with fetal bovine serum after passage. The primary cells of Dulbecco's modified Eagle's medium group were cultured with serum-free DMEM, changing the medium with fetal bovine serum after passage.

分别以下列血清微环境培养：自身血清组：分离细胞后原代用含大鼠自身血清的培养基培养，传代后换用含胎牛血清的培养基培养；同种异体血清组：分离细胞后原代用含同种异体血清的培养基培养，以后用胎牛血清培养基培养；胎牛血清组：分离后用含胎牛血清培养基培养，以后一直用含胎牛血清培养基培养；DMEM组：分离细胞后原代用不含血清的DMEM培养基培养，传代后换用含胎牛血清的培养基培养。

It is indicated that this loss will not change with the increase of the Reynolds number but the intensity of the passage vortex increases with the decrease of the Reynolds number. The tip leakage flow will weaken with the increase of the passage vortex because of the interaction between the passage vortex and the tip leakage vortex, and the position of the tip leakage vortex is more close to the middle of the passage. In addition, the distributions of the total pressure loss and the flow angle at the exit of the cascade are influenced by the intensity and the position of the tip leakage vortex.

结果表明：对于间隙存在造成的流动损失，数值模拟结果与实验结果及 Denton的预测值都十分接近，基本上不随着雷诺数的变化而变化；但是，随着雷诺数的减小，通道涡增强；间隙泄漏涡在和通道涡的相互作用中，强度减弱，在叶栅出口处的位置更加靠近中叶展；出口处的总压损失和气流角的分布也由于间隙泄漏涡强度和位置的变化而发生改变。

Compared with the corresponding BEAS-2B cells, the shapes of cells and nuclears, the shapes and quantity of cell organs were not significantly changed in the 1st, 2nd, 5th passage BEAS-2BNNK cells. The 10th, 15th passage BEAS-2BNNK cells, which had swelled cell and cell organs and abnormal nuclear and enlarged the nucleoli gradually and increased the quantity of the cell organs and activated their function, showed the characteristic of transformation cells. The 20th, 25th passage BEAS- 2BNNKcells, which had obvious aberration of the cell and nucleoli and had cataclasm of nucleoli and decreased cell organs, showed the characteristic of tumor cells.

与BEAS-2B相比较BEAS-2BNNK第1、2、5代细胞及胞核形态、细胞器形态及数量无明显差别；第10、15代细胞出现肿胀，细胞核逐渐变形，出现核畸形，核仁明显增大，边集，细胞器肿胀，数量明显增多，功能活跃具明显的转化细胞特征；第20、25代细胞及胞核明显畸变，出现明显的核碎裂及多个核仁，细胞器明显减少，具明显的肿瘤细胞特征。

RESULTS: The microscopic observation showed chondrocytes in the transducted group proliferated stably, and triangle, and spindle cells were predominant in the seventh passage, accompanied by few long-spindle and root cells. In addition, the cells were of clear appearance and large lucency. However, in the non-transducted group, long-spindle cells were observed in the fourth passage, and almost all cells exhibited long-spindle shape in the fifth passage.

结果：①倒置显微镜观察，转导组兔关节软骨细胞生长增殖稳定，至第7代仍旧以三角形、梭形及纺锤形细胞为主，偶见长梭形和树根形细胞，且轮廓清晰，透亮度大，折光性强；而未转导组细胞培养至第4代时即己出现长梭形细胞，自第5代细胞形态几乎全部变成长梭形。

The percentage of collagen type ⅱ and alcian blue positive stained rgcs decreased with increasing passage number. only the roundish cells of the 5th passage retained positive for collagen typeⅱ, and alcian blue staining of 3rd passage was negative, while α sma positive stained rgcs increased gradually.

随着传代次数的增加，阿尔新兰和ⅱ型胶原阳性染色的细胞比例迅速下降，第3代以后的rgcs阿尔新兰染色均为阴性，第5代rgcs也仅有圆形细胞仍保持了ⅱ型胶原阳性染色，而α sma阳性染色细胞的比例则不断增加。

Silk gland cell, by the Grace nutrient fluid in vitro culture, then after 150d for primary culture, passage was taken, later each approximately 15d take a passage, after that, the time of passage cut to 10 day.

经210d左右的原代培养后进行传代培养，以后每隔约15d传代一次，后传代时间缩短到10天，当传代时间稳定在7天时细胞开始趋于稳定，此时鉴定细胞系的生物学特性。

Adopt one kind of design plan that PLC controls in the protection the main body of a book has been introduced succeeding an electricity in electric system, that capital is designed time is that the KV circuit imports 10 mainly , change into 380 V voltage grades, before the PLC front and back add front to passage and the queen to passage, change before to passage middle course electric current , voltage a train of, the wave filtering the general is exchanged changing waking up with a start for direct-current, the entering carrying out comparison and switch amounts again , controlling conducting entering PLC, by PLC, The malfunction signal is shown out by lamp light or the alarm signal.

本文介绍了在电力系统继电保护中采用PLC控制的一种设计方案，本次设计主要是将10KV线路输入，转化为380V电压等级，在PLC前后加入前向通道和后向通道，在前向通道中经过一系列的电流、电压转换，将交流转换为直流，进行滤波，再进行比较和开关量的输入，传入PLC，通过PLC的控制，将故障信号通过灯光或者报警信号表现出来。

The maritime passage was a good augury for the aerial passage.

顺利的航海仿佛也是航空的好预兆。

Aer-+-ial 构成 The maritime passage was a good augury for the aerial passage.

顺利的航海仿佛也是航空的好预兆。

It is possible that houses were outrageously priced one year ago and that they are still priced much higher than the national average.

情况有可能是，这里的房价曾贵得离谱，现在的房价仍然远高于全国的平均指数。

This is a mother's love for her child.

这是母亲对孩子的关爱。