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The P32 gene of capripoxvirus were amplified by PCR.The product of PCR was cloned to the plasmid pGEM-T-Easy.Then the P32 gene was disparted to three parts.Three parts of P32 gene were inserted into the expressed plasmids pGEX-4T-1, which were identified by PCR and restriction enzyme analysis.

本研究利用PCR方法对羊痘病毒P32蛋白基因扩增，将回收PCR产物克隆到pGEM-T-Easy载体中，然后将P32蛋白基因分成3段，分别插入原核表达载体pGEX-4T-1中，经PCR和酶切鉴定，证明成功构建了重组表达载体pGEX-4T-1-N2/3、pGEX-4T-1-N1/3、pGEX-4T-1-M。

When dismantling the seal, remove the fixed plate that is fixed by screws on the inner surface of the rolling cylinder, and then draw out the two sealing equipments and the seal rings, and install the new seal gasket and seal parts and assure the perfect dovetailing of parts and no obstacle present.

拆除密封时，卸掉用螺钉固定在转筒端盖内表面上的固定板，然后拉出两个密封装置和密封隔离环，装进新的密封垫圈和密封件时，确认零件完全对准和无障碍。

This paper is composed of six parts, and emphasizes on four parts : emceeing personification, lansign, unlansign and play-by-play state. It is expected to anatomise conventional function of comperes" individual charms that aim at people" intercommunion in TV transmission through communication subject and other subjects.

本文共六个部分，将重点论述主持人格化、语言符号与非语言符号以及播出状态这四部分，以求从传播学以及其它学科的角度着手剖析主持人的个性魅力在电视以建立人际交流为目标的传播过程中所起的约定性作用。

The resulting composites are used in rotor blades for helicopters, compressor blades in jet engined,golf club shafts, bicycle parts, skis, and machninary parts.

碳纤维可以用来生产具有防火功能的织物。

Great progress has been made by taking the following technical improvement for the 2500 ton Hydropress: to replace thin oil with lithium grease in its lubricating system; to add four manual grease pumps; to lubricate the four griding parts of the movable beam and the six parts of the column plug sleeves.

通过对2500吨水压机润滑系统改稀油润滑为锂基润滑脂润滑，加装四台手动干油泵，对活动梁四个导向部位和主柱塞压套六个部位实施油膜润滑的技改实践，使1995年较1994年设备完好率提高1。

Stanley Kauffmann said of the Coens:''Their jumbling of tones makes the grim parts harder to credit and makes the funny parts seem like old-fashioned comic relief.'' I disagree.

史丹利考夫曼(译注，米国作家，影评人)就评价科恩兄弟&不停变换腔调让该吓人的部分不吓人，该好笑的地方又成了简陋的气氛调节。&

This is particularly useful for protecting manufactured parts, from delicate medical components to packing of industrial machined parts.

这是特别有用的地方，从保护微妙的制造医疗元件，以工业机械零件包装。

The research begins with meliorating the basic elements--buildings andbuildings′layout in environments of parts of old campus. and tries to get a strategyrenewing the resources of old campus which ranges from parts melioration to wholeoptimization , under the conditions of making the most of old buildings, environmentsand structures.

研究从旧校园中的基本单元——局部环境内的建筑实体以及建筑布局改善入手，在对旧建筑、旧环境、旧格局充分利用的前提下，试图寻找一种在整体协调基础上，由局部改善到整体优化的旧校园资源更新对策。

Through analysis of the oil-seat oiling parts of the structure and characteristics of the processing requirements of the development of a more reasonable fixture design, so as to guarantee the precision machining of parts will provide an economical and practical technology equipment, has some practical value.

本文通过分析注油器油阀座零件的结构特点和加工要求，制定了一套较合理的夹具设计，从而为保证该零件的加工精度将提供一种经济实用的工艺装备，具有一定的实用价值。

The article opines that a systemic cash flow management theory should consist of three parts: basic theory of cash flow management, applied theory of cash flow management and cash flow management theory in special industry or special business. The article tentatively discusses the element issues related to the former two parts in relatively full scale.

本文提出系统的现金流量管理理论体系应由现金流量管理基本理论、现金流量管理应用理论和特殊行业或特殊业务现金流量管理理论三部分组成，并对前两者所涉及的基本问题进行了初步的、较为全面和系统的探讨。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。