查询词典 paraformaldehyde

Acquisition of high-quality chromosome suspension: The chromosome suspension released from homogenization suited for flow karyotypic analysis and sorting after fixed in 2% paraformaldehyde for 20 minutes and purified through 10% and 30% sucrose concentration gradient.

高质量染色体悬液制备结果：同步化的根尖细胞经2%多聚甲醛固定20 min，机械匀浆释放染色体并结合10%及30%蔗糖浓度梯度纯化释放的染色体悬液。

At 5 hours, 10 hours, 1 days, 2 days, 3 days, 1 week and 2 weeks after the operation, animals were perfused from the heart with a fixative containing 4% paraformaldehyde in 0.1 M phosphate buffer.

而在脑损伤部外侧2－4mm区域的大脑皮质，组织结构清楚，血管旁的间藤细胞，因吞入了大量的HRP，而清晰可见。

The chest of fetal animals was opened, and the tissue was fixed with 4% paraformaldehyde at 4°C. Lungs from postnatal animals were inflation fixed at 25 cmH2O pressure via a tracheal cannula with the same fixative.

胸部胎动物开幕式，并组织固定， 4 ％甲醛在4 ℃的温度肺从产后的动物通货膨胀率定为25 cmH2O 压力通过气管插管同固定。

MAIN OUTCOME MEASURES: The seeded cells were observed under inverted microscope; at 1, 2, 3 weeks after seeding, the BMSCs were treated with 4% paraformaldehyde and stained with hematoxylin-eosin; The protein content in seeded cells was determined by bicinchoninic acid assay, and the content of DNA was quantified using Hoechst33258 assay at 5, 10, 14 days after culture.

主要观察指标：倒置显微镜下观察细胞形态；于培养1，2，3周采用40 g/L多聚甲醛固定，常规组织切片，苏木精-伊红染色；在培养5，10，14 d用Hoechs33258荧光法定量测定细胞内DNA含量，BCA法测定蛋白质含量。

The optimum dyeing conditions were shown: viral samples fixed with 0.1% paraformaldehyde for 30 min at 4℃, stained with SYBR Green I 1 × 10^(-4 commercial concentration for 10 min at 80℃.

杆状病毒最佳染色条件：质量分数为0.1%的多聚甲醛固定病毒30min后，加入一定量的SYBR Green I在80℃下孵育10min。

MethodsAfter fixed by 4% paraformaldehyde and embedded in paraffin,retinas of 26 healthy adult Wistar rats were studied by immunochemistry and digital photomicrograph analysis to detect the locations and levels of the expression of SDF-1 and pan cytokeratin.

本研究的主要目的在于了解生理状况下SDF-1在成年大鼠视网膜上的表达情况，为眼内新生血管

Abstracts The effects of fixation with paraformaldehyde or glutaraldehyde and preservation with liquid nitrogen on cell size, density, phycoerythrin fluorescence value (FL2) and chlorophyll fluorescence (FL3) of picoplanton cells were studied by using flow cytometry.

摘要以流式细胞计为海洋微微型浮游生物的分析工具，研究了醛类固定剂的固定、液氮贮存对于微微型浮游生物的细胞大小、密度、藻红素荧光值(FL2)和叶绿素荧光值(FL3)的影响。

Ethyl dicyanopropionate was synthesized by one-step reaction with sodium cyanide, paraformaldehyde and ethyl cyanoacetate in solvent.

由氰化钠、多聚甲醛和氰乙酸乙酯在溶剂中反应，一步合成二氰基丙酸乙酯，收率达80.2%，含量90.8

Different doses (25,50 and 100 mg/L)of gentamicin were add to the medium of SGC,and cultured for 14 days.After that,the specimen was fixed with 4%paraformaldehyde and stained with toluidine blue.

为观察硫酸庆大霉素对培养的小鼠耳蜗螺旋神经节细胞的细胞生存数和细胞突起长度的影响，在单离培养的出生1-5 d的NIH小鼠SGC培养液中，加入不同浓度的硫酸庆大霉素(25，50，100 mg/L)。

METHODS: The rats in the two groups were anaesthetized by intraperitoneal injection of 10 g/L urethane (1 g/kg) and the chest was opened to insert a tube into the ascending aorta for perfusion with 200 mL of the mixture containing glutaric dialdehyde and paraformaldehyde for fixation.

两组大鼠给予质量浓度为10 g/L的乌拉坦1 g/kg腹腔内注射，麻醉后开胸，升主动脉插管，戊二醛与多聚甲醛混合固定液200 mL灌流固定，取嗅球，振荡切片，锇酸后固定，平板包埋，光镜下选取嗅球各层，制备超薄切片，透射电镜下对比观察。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。