查询词典 pap

No mediastinal and hilar adenopathy was found. Conclusion The characteristic appearances of PAP on MSCT correspond to its pathological features.The "crazy paving" sign in geographical areas of pulmonary opacification greatly suggests PAP diagnosis.

本文收集经病理证实的6例PAP，综合国内外有关文献，探讨PAP的影像病理学联系及其多层CT表现特征，旨在提高对本病的认识和诊断。

Pokeweed antiviral protein belongs to Ribosome-inactive Proteins. PAP has potent antiviral activity against many plant virus. pT1 was used as the template to amplify the deleted mutant PAP gene (N-deleted and N-,C-deIeted) by PCR and then the two genes were cloned into expression vector. Different IPTG-inducible expression vector containing different deleted mutant PAP gene was constructed and transferred into E.coli strain BL21 (DE3)-plysS.

美洲商陆抗病毒蛋白是一类能很好的抑制植物病毒的蛋白，很早就有报道低剂量的商陆叶片粗粉末就能明显的抑制TMV在菜豆叶片上局部枯斑的形成，PAP缓释剂也已用于病毒病的防治工作，但美洲商陆抗病毒蛋白属于核糖体失活蛋白，它对生长的细胞会有一定的毒性。

Transgenic tobacco plants were obtained through screening with kanamycin. The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants. Pokeweed antiviral protein Ⅱ is expressed with high level in summer leaves. The expression of PAPⅡ is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPⅡ gene by RT-PCR and then the gene was cloned into E. coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPⅡ gene were then transferred into E. coli strain BL21 (DE3)-plysS and Pachia pastoris GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

The nucleotide sequences of the PAP gene and PAPⅡ gene in the root of Phytolacca americana were determined by reverse transcription polymerase chain reaction and PCR. The gene comprises 942bp and 933bp respectively, encoding 313 amino acids and 310 amino acids. When PAP gene and PAPⅡ gene sequences in the root were compared with those in the spring leaves and summer ones, the highest similarity 100% and 99. 8% were observed respectively. They both had one open reading frame . Analysis of the two sequences showed, in fact, they belonged to one species.

以美洲商陆根为材料，提取其总RNA、反转录，用一对PAP基因的特异性引物和一对PAPⅡ的特异性引物，分别对反转录产物进行PCR扩增，将扩增的目的片段进行序列分析，结果扩增出来的PAP基因与已经报道的序列完全相同，扩增出来的PAPⅡ基因与已经报道的序列同源性也在99.8％，而且它们都各自编码一个开放阅读框架，可以说美洲商陆根中肯定存在PAP和PAPⅡ基因，并已经转录。

The nucleotide sequences of the PAP gene and PAPII gene in the root of Phytolacca americana were determined by reverse transcription polymerase chain reaction and PCR. The gene comprises 942bp and 933bp respectively, encoding 313 amino acids and 310 amino acids. When PAP gene and PAPII gene sequences in the root were compared with those in the spring leaves and summer ones, the highest similarity 100% and 99.8% were observed respectively. They both had one open reading frame. Analysis of the two sequences showed, in fact, they belonged to one species.

以美洲商陆根为材料，提取其总RNA、反转录，用一对PAP基因的特异性引物和一对PAPⅡ的特异性引物，分别对反转录产物进行PCR扩增，将扩增的目的片段进行序列分析，结果扩增出来的PAP基因与已经报道的序列完全相同，扩增出来的PAPⅡ基因与已经报道的序列同源性也在99.8％，而且它们都各自编码一个开放阅读框架，可以说美洲商陆根中肯定存在PAP和PAPⅡ基因，并已经转录。

The deleted mutant PAP gene was also cloned into yeast secreted expression pPIC9K vector to form pPIC9K~3, then the vector was transferred into Pachia pastoris GS115 strain. The specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50-60 U g per millilitre measured by UV-absorbed methods in the supernatant of the medium via high density fermentation. SDS-PAGE results showed that there was one protein band in the gel which molecular weight was about 34Ku . The protein could specific react with France PAP antiserum in Western-blotting. Activity tests revealed t

将缺失型PAP基因克隆于酵母分泌型表达载体PPICgK构成重组载体，然后导入毕赤酵母（P8chia nastoris）菌株GSlls细胞中，在甲醇的诱导下，经过酵母高密度发酵进行PAP的表达，经SDS－PAGE分析，结果表明，在培养基上清液中含有一明显的特异性蛋臼条带，大小为34Ku，经Western－blotting分析，该蛋白与法国PAP抗血清有特异性反应，体外活性检测表明该蛋白对TMV的侵染性具有高度的抑制性，说明该 PAP基因在毕赤酵母 GS中也得到了正确表达。

This developed a new material for the wheat virus-resistance breeding.In order to breed maize dwarf mosaic disease梤esistance maize species, the PAP gene was introduced into maize inbred strains and hybrid strains mediated by pollen and particle bombardment,10 transgenic maize plants were achieved and the PAP gene was indeed integrated into the maize genome by analysis of molecular biotechnological methods. The PAP gene in one of the transgenic maize plants had been transcription and translated, the ability of the virus resistance is undergoing.

为了解决玉米矮花叶病害的防治问题，采用花粉介导法及基因枪法将PAP基因分别导入玉米的杂交种及自交系，获得了10株转基因玉米，PCR及Southern-blot检测结果表明PAP基因已经整合到了玉米基因组中，并且其中有一株PAP基因已经得到了表达，病毒抗性鉴定表明转基因玉米能明显推迟玉米矮花叶病害症状的表现。

Results All the 6 patients with PAP presented groundglass opacities with welldefined margins on MSCT'creating "geographic" pattern'reflected the effusion of proteinaceous material within the alveoli in PAP.

回顾性研究我院2000年1月～2005年6月经病理学证实的6例PAP的多层CT表现，并与病理学改变相对照，探讨PAP的影像病理学联系及多层CT表现特征。

The characteristic appearances of PAP on MSCT correspond to its pathological features.The "crazy paving" sign in geographical areas of pulmonary opacification greatly suggests PAP diagnosis.

PAP的多层CT表现能够反映其病理学特征，边界清楚的毛玻璃样高密度影，呈地图样及铺路石样表现能高度提示PAP诊断。

Green fluorescent protein gene was conjugated to the 3 end of the PAP gene in order to screen easily of the transgenic cotton plants. The combined gene was cloned into plant expression vector pBI121 and then transformed. About 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays. This result reflected that the GFP gene was expressed in the transgenic cotton; dot-blot result also demonstrated that PAP gene was exactly transformed into the cotton. The capacity of the Verticillium dahliae-resistance is being analyzed.

为了便于转基因棉花后代的筛选，在 PAP基因的 3'端融入了绿色荧光蛋白GFP）基因，然后将融合基因克隆在植物表达载体PBI 121上，再进行遗传转化，得转基因棉花种子5000余粒，将种子播种长到于叶展开时，先在黑暗中用紫外灯照射，查找表现绿色荧光的幼苗，然后再用地高辛标记的PAP基因特异性探针对这些棉花进行点杂交，最后发现有8株棉花表现阳性反应，说明PAP基因的确己经转到了棉花的基因组中，其棉花黄萎病的抗性鉴定正在进行之中。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失