查询词典 pair of

One pair of primers that amplified the gB gene of pseudorabies viruswas designed and synthesized.PCR technique detecting the DNA of PRV was established after selecting the best reaction conditions.This technique was applied to specifically amplify the 281 bp DNA fragment of the PRV strains including Fa,Fb,Bartha,BJ,GD,V2F4,S,S3,SR,Buk,Shope,Norden,Mink Ⅲ,HB,F8,F9 and F12 in cultured samples.The negative results were achieved from Vero cells,swine vesicular disease virus,hog cholera virus,Japanese encephalitis virus,porcine reproductive and respiratory syndrome virus,porcine parvovirus,foot and mouth disease virus F29 strain,O3I3 strain,T509 strain and O Ⅱ MF249 strain.The results of sequencing showed that the PCR method was of specificity.The sensitivity of PCR reached 15.8 pg of PRV Fa strain DNA.The tissue samples obtained during 1994 and 2000 were detected,and the results showed that the sensitivity of PCR was more sensitive than virus isolation and the Sandwich ELISA.The PCR was applied to detect 191 tissue samples from 31 pig farms obtained from Guangdong,Fujian,Hainan Provinces during 1999 and 2000,50 samples(26.2%)were positive and 22 pig farms(71%)were positive.

根据伪狂犬病病毒gB基因的序列，设计并合成了一对引物，以闽A株细胞培养毒为模板，筛选最佳反应条件，建立了检测PRV的PCR方法应用该方法对Fb、Bartha、BJ、GD、V2F4、S、S3、SR、Buk、Shope、Norden、MinkⅢ、HB、F8、F9、F12等毒株的细胞培养液进行基因扩增，均获得了分子量为 2 81bp的特异性目的DNA片段，而对Vero细胞与FMDV、SVDV、HCV、PRRSV、JEV、PPV等病毒进行检测，结果均为阴性，没有出现交叉反应对PRV毒株扩增的产物测序，结果序列与文献报道一致，证明PCR扩增产物和方法的特异性对 1994～ 2 0 0 0年期间送检的临床样品和保存的PRV毒种，用病毒分离、双抗体夹心ELISA和PCR等 3种方法进行检测，结果前 2种方法检测为阳性的，PCR检测均为阳性；PCR检测为阴性，前 2种方法检测也为阴性；可是，前 2种方法检测为阴性的，PCR却检测出部分阳性；经x2 检验，证明PCR检出率明显高于前 2种方法的检出率对PRV闽A株细胞毒提取物DNA进行检测，其最低检出量为 15 8pg 对 1999～ 2 0 0 0年期间广东、福建、海南等省的 31个大中型猪场送检的 191份病料进行检测。

Factor, decide at the pack head to go to Xian to build 1 lately sets up a railroad, this engineering is a pair of track circuit with total goods, design soon the 250 Kms/h , in set up of yellow river especially big bridge being located on this circuit, cross over yellow river length to reach to a 3912.89 ms, in especially the construction of the big bridge, measure the work show of is importance, relate to whole bridgeses of run-through, measure in use the whole station instrument and intelligence calculator, carry on a precision to measure to establish to the point, come to a to show Zhao in actually the work of effect, and speed construction progress, economized cost to create a performance.

为了适应经济的发展，进一步的开发大西北，也为了缓解内蒙古包头及东胜地区煤炭资源外运的运力紧张问题，结合当地的经济技术地质等因素，决定在包头至西安修建一条新建铁路，本工程为双线客货共轨线路，设计时速250Km/h，在建的黄河特大桥位于本线路上，跨越黄河长度达3912.89m，在特大桥的建设中，测量工作显的尤为重要，关系到全桥的贯通，测量中使用全站仪及智能计算器，对点位进行精确测设，在实际工作中达到了显著的效果，而且加快了施工进度，节约了成本创造了效益。

Cable .Make use of this principle, we can quickly locate the target cable or break point from large amount of cables, under carpet, the inner of decoration wall and over ceiling1 operation is simple , reliable , the interface is nimble , use is broad , bionic form , carry-over are convenient, since our link tester has two signal output port, it can work when switch is running, this is very convenient. 2 applies to all metal cable such as network cable , tele phone line cable , video cable , automobile circuit .it can not be work with high voltage(above 80V),if you want to locate it ,please turn off the cable power supply3 can test the telephone line polarity, connectedness, rough position of breaking point.4 uses the two crocodile clamp to avoid getting rid of the job of make network cable. Use the RJ45/RJ11 interface can connect to the network and the telephone line directly.5 link tester can find out that pair of circuit line cable from large mount of cables. 6 high amplitude ranks 200 times amplification circuit is sui table to locate long distance cable.7 two-sided network lines sequence indicator is easy to examine the network line cable whether or not correctly conveniently. 8 portable types multifunctional bag can encase most of your engineering tools; remit your messy carry-over of implement bothering. 9 adopt two 9V batteries.

线路通产品特点：1 操作简单、性能可靠，接口灵活、用途广泛，仿生外形、携带方便，由于本设备采用了信号双路输出方式，可以解决交换机工作状态下使用，避免了关掉交换机影响其他线路的使用的麻烦。2 适用于查找网络电缆、电话线路、视频电缆、汽车电路、无强电状态下的所有金属电缆。3 可以测试电话线路极性、连通性，断点大概位置。4 两个鳄鱼嘴夹，避免了去做水晶头的麻烦，RJ45/RJ11接口线，可直接连接网络和电话线模块。5 寻线器快速地在众多紊乱的线路中找出需要查找的那一对线路线缆。6 高音频振幅级别200倍的放大电路，适合长距离电缆定位。7 双面网络线续灯可以更直观方便的检查校正网络线续线路连接是否正确。8 便携式多功能软包，可以把您工程施工、维护的基本工具装齐，免除您工具散乱携带的麻烦。9 本寻线器采用2节9V电池。

Be work with high voltage(above 80V),if you want to locate it ,please turn off the cable power supply3 can test the telephone line polarity, connectedness, rough position of breaking point.4 uses the two crocodile clamp to avoid getting rid of the job of make network cable. Use the RJ45/RJ11 interface can connect to the network and the telephone line directly.5 link tester can find out that pair of circuit line cable from large mount of cables. 6 high amplitude ranks 200 times amplification circuit is suitable to locate long distance cable.7 two-sided network lines sequence indicator is easy to examine the network line cable whether or not correctly conveniently. 8 portable types multifunctional bag can encase most of your engineering tools; remit your messy carry-over of implement bothering. 9 adopt two 9V batteries.

l线路通产品特点：1 操作简单、性能可靠，接口灵活、用途广泛，仿生外形、携带方便，由于本设备采用了信号双路输出方式，可以解决交换机工作状态下使用，避免了关掉交换机影响其他线路的使用的麻烦。2 适用于查找网络电缆、电话线路、视频电缆、汽车电路、无强电状态下的所有金属电缆。3 可以测试电话线路极性、连通性，断点大概位置。4 两个鳄鱼嘴夹，避免了去做水晶头的麻烦，RJ45/RJ11接口线，可直接连接网络和电话线模块。5 寻线器快速地在众多紊乱的线路中找出需要查找的那一对线路线缆。6 高音频振幅级别200倍的放大电路，适合长距离电缆定位。7 双面网络线续灯可以更直观方便的检查校正网络线续线路连接是否正确。8 便携式多功能软包，可以把您工程施工、维护的基本工具装齐，免除您工具散乱携带的麻烦。9 本寻线器采用2节9V电池。

At that period a dandy was composed of a tall collar, a big cravat, a watch with trinkets, three vests of different colors, worn one on top of the other--the red and blue inside; of a short-waisted olive coat, with a codfish tail, a double row of silver buttons set close to each other and running up to the shoulder; and a pair of trousers of a lighter shade of olive, ornamented on the two seams with an indefinite, but always uneven, number of lines, varying from one to eleven--a limit which was never exceeded.

在那时代，一个佳公子的组成部分是一条高领、一个大领结、一只珠饰累累的表、一叠三件蓝红在里的颜色不同的背心、一件橄榄色的短燕尾服、两行密密相连一直排列到肩头的银钮扣、一条浅橄榄色裤子，在两旁的线缝上，装饰着或多或少的丝曾超过的限度。

A self-converse Mendelsohn packing design of order v, denoted by (v, k,1)-SCMP, is defined to be a triple, where B is a collection of k-circuit of X such that every ordered pair of distinct points of X appears in at most one block of B, and / is a permutation on X such that Here, and whenB=(x0 ,x1,xk-1). The number of blocks in a (v, k, 1 )-SCMP is upperbounded by where 1 if v=l ande =0 , otherwise.

一个阶为v，区组大小为k的自反Mendelsohn填充设计，记为—SCMP，定义为一个有序三元组，其中X为v元点集，B为X的一族循环有序k元组，f为X上的一个置换，它满足如下性质：(1)对任意x，y∈X，x≠y，有序对至多包含在一个区组中；这里区组当且仅当和一个—SCMP所含的区组个数不超过，其中当V(v-1)≡1时，ε=1；否则ε=0。

Turn over the Ji Cape of winding backward like the cloud line sort and again long again of the lion nose of Kuo, is a pair of months long the stone sort is suffused with green white ray of light of eyes.

如同云纹般向后翻卷的虬角和又长又阔的狮鼻之间，是一双月长石般泛着青白色光芒的眼睛。

To take one especially spectacular example: A traditional paisley pattern was embroidered on the sleeves of a coat, then it trailed off down the leg of the matching trousers before reappearing on the lapels of a suit and the shoulders of a sweater (which also featured a leopard-print collar). Here's another illustration: Sheeshedar, the mirrored Indian fabric you've seen in markets since time immemorial (or at least since hippies crossed the landmass from Asia to America), was used to make a pair of trousers—and matching shoes. Then it was teamed up with a poncho that could have been sliced from shaggy white yak wool. Just perfect for the backstage area of a Led Zeppelin reunion concert, but curiously incongruous for a McQueen fashion moment. Perhaps that was the problem with this show.

最明显的表现部分就是：古波斯国的传统涡纹花呢被设计师运用细腻精致的刺绣织造手法，展露在外套的袖口、西装长裤的小腿肚、西装外套的翻领、以及毛衣的双肩部份，（还有美洲豹皮纹也被添加在领口处）；同时，镜面反射般的立体明暗光泽也被McQueen加强在成套的裤装/鞋款上，那种神秘的异国光泽，年代攸远似乎已不可考，但早期曾在嬉皮年代被一些摇滚乐手从印度辗转传至美洲和英国；不过可惜像是南美风格的白色堥牛毛poncho披风，却被评为，似乎适合Led Zeppelin演唱会后台的歌迷们穿著、而极不搭调在McQueen的fashion how现场。

This will vary with different natures, but this is the place where a wise man will dig his cellar…… I one evening overtook one of my townsmen, who has accumulated what is called "a handsome property"—— though I never got a fair view of it —— on the Walden road, driving a pair of cattle to market, who inquired of me how I could bring my mind to give up so many of the comforts of life.

人的性格不同，所以需要也很不相同，可是一个聪明人必需在不竭之源泉的大自然那里挖掘他的地窖……有一个晚上在走向瓦尔登湖的路上，我赶上了一个市民同胞，他已经积蓄了所谓的&一笔很可观的产业&，虽然我从没有好好地看到过它，那晚上他赶着一对牛上市场去，他间我，我是怎么想出来的，宁肯抛弃这么多人生的乐趣？

The method is just like the QuikChange site-directed mutagenesis method except for its special primer. This pair of primers is composed of a long primer and a short primer which is central overlapped.Both of the Tm of 5\'sequence in long primer and Tm of 3\'sequence in short primer are above 66℃.The Tm of short primer is almost half of that of long primer.It is convenient to perform insertion or substitution mutation of DNA fragments larger than 20bp,In addition,it is highly applicable in research about the deletion mutation of DNA fragments larger than 2000bp.

我们实验室一直在寻找一种简单高效的可以用于基因的删除、插入及替代突变的方法，通过大量研究和实验发现，可以使用一种基于反向PCR原理，使用全新的引物设计的突变方法——&COP&突变法，该方法采用与QuikChange点突变法相同的操作步骤，其策略是设计一对中心重合的引物，长引物的5\'端序列和3\'端序列的Tm值均不低于66℃，短引物Tm值约为长引物一半，且与长引物中心重合互补。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。