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To establish the base of the control of the Citrus aurantium L. fruit maturity in molecular level and produce high quality Fructus Aurantii, a pair of degenergte primers were designed according to the conserve regions of ACC Oxidase gene of many kinds of plants.

根据已报道的各种植物ACC氧化酶氨基酸序列上前后两个保守区设计1对简并引物，以酸橙叶片总RNA为模板，通过RT-PCR扩增到1个834bp的基因片段。

Edit in what defend Jian measure respect, the main opinion that gathers before this includes: It is to suggest not to lengthen the orgnaization that protect Jian to doing poineering work board the superintend and director of the company guides period, card inspect can think through research, because do poineering work board company dimensions is relatively lesser, management stability is relatively inferior, lengthen superintend and director to guide appropriately period be helpful for active and reliable roll out do poineering work board, reason did not grant to adopt; 2 it is to suggest exempt does poineering work board company IPO in those days namely the responsibility of the person that protect Jian of deficit, card inspect can think via research, although do poineering work board the company is managed not stable, but if the company appears on the market in those days with respect to deficit, can produce baddish effect to the market, because this cannot loosen the responsibility of pair of people that protect Jian to find out; 3 it is to suggest to unlock person of the delegate that protect Jian to sign odd number to measure limitation, meeting general is keeping next card watch in be being arranged related Jian system, give further research, hear opinion of orgnaization of Jian of be bailed out, make arrangement jointly.

在保荐办法的修订方面，此前收集到的主要意见包括：一是建议不要延长保荐机构对创业板公司的督导期，证监会经过研究认为，由于创业板公司规模相对较小，经营稳定性相对较低，适当延长督导期有利于积极稳妥推出创业板，故未予采纳；二是建议豁免创业板公司IPO当年即亏损的保荐人责任，证监会经研究认为，尽管创业板公司经营不稳定，但如果公司上市当年就亏损，对市场会产生较坏的影响，因此不能放松对保荐人的责任追究；三是建议放开保荐代表人签单数量限制，下一步证监会将在保荐制度相关安排中予以进一步研究，听取保荐机构意见，共同做出安排。

The effective stimulating sites were indentified by defensive cardiovascular response and other components of defence reaction evoked by 3 s trains of rectangular cathodal pulses (100Hz, 0.5ms pulse duration, 70 -150μA intensity for dPAG and 150-250μA for PF) delivered from a programmed multichannel constant current stimulator. The responses included an increase in blood pressure and heart rate, pupillodilation and an increase in the rate and depth of respiration. Currents of 1Hz, intensity of 100- 150μA for dPAG and 200-250μA for PF were used to observe the effect of the stimulation of them on RVLM neurones. The deep peroneal nerve and superfacial deep peroneal nerve were carefully separated, and put on a pair of stimulating electrode respectively, Both nerves were stimulated with constant rectangular pulses of 1Hz, 0.5ms duration and 300μA current intensity to see their effects on RVLM neurones. The baro-and chemo-receptors were activated by the intravenous injection of phenylephrine or 0.208 M NaH2PO4. The degree of convergent inputs to individual RVLM neurone was studied by the stimulation of the sites mentioned above. It was found that neurones about 61.4%(27/44) and 35.3%(6/17) received convergent inputs from three and four places respectively.

过去的研究主要集中于整体水平，利用电刺激或化学刺激RVLM神经元，观察对血压、心率及交感活动的影响，并对影响心血管交感活动的神经递质进行了广泛探讨，积累了大量资料；在神经元水平，主要研究刺激脑内核团、外周神经、化学感受器及压力感受器等对RVLM神经元放电的影响和某些神经递质影响神经元活动的机制、然而，对RVLM神经元如何整合不同信息、其可能突触机制等研究得较少，本互作拟采用在体细胞内、细胞外记录电活动与微电泳，结合中枢核团及外周神经电刺激等方法，对RVLM神经元在不同状态下的整合作用规律与可能机制进行初步探讨，为进一步了解这一复杂的整合过程提供新的理论依据。

According to the alignment analysis of full length sequences of Cytb gene from closely relate species of Cervus , one pair of allele-specific diagnostic PCR primers was designed. Factors such as annealing temperature, dosage of polymerase, times of cycles and dosage of template DNA that influence the PCR results were also investigated.

根据鹿科鹿属种动物Cytb全序列比对分析，设计1对能准确鉴别正品鹿茸和其他近源种鹿茸的引物，在此基础上建立鹿茸药材位点特异性PCR鉴别方法，并对影响PCR结果的主要因素退火温度、 Taq 用量、循环次数以及模板用量和重复性等进行方法学考察和优化。

Analyse and compare what adopt a variety of pair of methods, the Michael addition reaction of ester of acerbity second of second of hydroxyl of protection of benzyl of the response of close nucleus addition that uses second nitrile and ester of formic acid second certainly, chloridize, cyanogen, reaction that take off carboxyl generates 3- benzyl oxygen radical the fifth of the ten Heavenly Stem the method of 2 nitrile, detailed discussion the action that wait and the by-product that arise possibly reach the reactant in each pace reaction, activator, dissolvent avoid a method.

通过对多种方法的分析和比较，确定采用乙腈和甲酸乙酯的亲核加成反应、氯化苄保护羟基、氰乙酸乙酯的Michael加成反应、脱羧反应生成3-苄氧基戊二腈的方法，并具体的讨论了每一步反应中反应物、催化剂、溶剂等的功能和可能产生的副产物及避免方法。

When doing not have media, can snarl of be laughing and playing, when facing medium, overcautious, all without exception explains, partial delegate has level of a speech in commissarial heart: And decide this kind of level, it is the understanding of pair of their duty not just, it is to be based on the value of a kind of be swayed by considerations of gain and loss to accept or reject more.

没有媒体时，可以嬉笑怒骂，面对媒体时，则谨小慎微，无不说明，部分代表委员心里有一个发言标准：而决定这种标准的，则不仅仅是对他们职责的理解，更是基于一种患得患失的价值取舍。

This paper passes one word of pair of " law " to be in the use state of Sino-Japanese two countries, form the respect such as the process make an on-the-spot investigation, made clear a vocabulary use a relationship, the form of the culture setting that discussed Sino-Japanese two countries to collect communication and interference in recent statement and latter-day Chinese vocabulary is didymous the influence of the circumjacent country that is a delegate with peninsula of Japan, Korea.

本论文通过对&法律&一词在中日两国的使用状况、形成过程等方面的考察，明确了词汇的借用关系，探索了中日两国在近代词汇交流和干涉的文化背景以及近代汉语词汇的形成对以日本、朝鲜半岛为代表的周边国家的影响。

"3 Delegates " serious thought has directiveness of sex of sex of innovation of historical inevitability, times, objective truth and practice on Marxism phylogeny, be theory of pair of Marxism-Leninism, Mao Zedong Thought, Deng Xiaoping accede and develop, it is the newest and academic positive result that Marxism China turns.

&三个代表&重要思想在马克思主义发展史上具有历史必然性、时代创新性、客观真理性和实践指导性，是对马列主义、毛泽东思想、邓小平理论的继承和发展，是马克思主义中国化的最新理论成果。

IT者网站提供英文翻译,未经授权请勿转载】 On May 21 afternoon, dozenth the prize-giving ceremony of selection activity of own innovation of the 3rd China is in an exposition of industry of science and technology of Chinese Beijing international Beijing is held, regard a nation as the delegate of software, golden hill software will " own innovation is outstanding brand award " bursa is obtained, this also is pair of golden hill the 20 affirmation that will hold to own innovation for years.

5月21日下午，第十二届中国北京国际科技产业博览会第三届中国自主创新评选活动的颁奖仪式在北京举办，作为民族软件的代表，金山软件将&自主创新卓越品牌奖&囊获，这也是对金山20多年来坚持自主创新的肯定。

Nevertheless Guangdong guides Yong Gang of Cui of attorney office solicitor tells a reporter, from the point of current law, if do not have the information such as the calumniatory, baleful attack that issues pair of enterprises clearly on the net, 315 websites of folk of very difficult judgement are announced complained behavior and trade to whether have extortionary ill will for what this and enterprise happen, because of such, ability can have so many 315 websites to exist.

不过广东导正律师事务所律师崔勇刚告诉记者，从目前的法律来看，如果没有明确在网上发布对企业的诽谤、恶意攻击等信息，很难判断民间315网站公布投诉的行为和为此与企业发生的交易是否有勒索之嫌，正因如此，才会有如此多的315网站存在。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。