查询词典 page

Using SDS-PAGE technique, HMW-GS of Thinopyrum were detected by analyzing Thinopyrum accessions, wheat-Th. elongatum 1E〓 substitution lines and wheat-Th. intermediam partial amphidiploid Zhong2. It was showed that the composition of HMW-GSs of Thinopyrum were distinctly different from which in wheat.

对偃麦草、二倍体长穗偃麦草1E中国春代换系、小麦—中间偃麦草部分双二倍体中〓高分子量谷蛋白亚基进行了SDS-PAGE分析，发现在偃麦草材料中存在不同于小麦的特异HMW-GS类型。

Results Agarose gel electrophoresis proved that the length of PCR product was about 7 000 bp. SDS-PAGE showed that the target protein was highly expressed. Compared with those of control, the anthranilate synthase and tryptophan synthase activities of expressed product increased by 2.7 and 3.2 folds respectively.

结果 凝胶电泳可见代PCR扩增产物大小约为7000bp，SDS-PAGE鉴定目的蛋白分别得到了表达，邻氨基苯甲酸合成酶和色氨酸合成酶的活性分别比对照提高了2.7和3.2倍。

It was homogenous as judged by SDS-PAGE and had an apparent molecular weight of 36 kDa under non-reducing conditions and 38 kDa under reducing conditions, respectively.

Jerdonitin在还原和非还原SDS-PAGE电泳中分别呈现一条表观分子量为38和36 kDa的条带。

After positive clones were induced by L arabinose for 4 hours, the purity of protein was detected by SDS PAGE and its bioactivity was identified by competitive inhibition ELISA test.

阳性克隆用左旋阿拉伯糖诱导4 h， SDS PAGE电泳检测蛋白纯度，竞争抑制ELISA实验检测其活性。

He was recruited into the band in 1968 by Jimmy Page at the suggestion of lead singer Robert Plant.

约翰是在 1968 年经由主唱 Robert Plant 的建议，而由 Jimmy Page 招募入团。

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

Vegetative storage proteins in the Melia azedarach L. were identified by using optical microscopy and SDS-PAGE.

以不同季节的苦楝枝条为研究对象，利用光学显微技术和SDS-PAGE技术研究其枝条中贮藏蛋白在休眠期和生长期的分布情况。

Compared with SDS-PAGE results of the development season barks, there were six 12, 30, 32, 38, 40, 53 kD major proteins expressed in the dormancy season barks, which was probably the vegetative storage protein of Melia azedarach L.

SDS-PAGE结果表明：休眠期枝条中的蛋白条带数目明显多于生长期枝条且浓度高，有12、30、32、38、40、53kD 6条蛋白带在休眠期枝条细胞中大量特异表达。

Vegetative storage proteinsin the Melia azedarach L.were identified by using optical microscopy and SDS-PAGE.

以不同季节的苦楝枝条为研究对象，利用光学显微技术和SDS-PAGE技术研究其枝条中贮藏蛋白在休眠期和生长期的分布情况。

Michael Page's approach goes for bathos.

Michael Page追求意想不到的效果。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。