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The purity identification was made by SDS-PAGE,absorption spectrum and amino acid analysis.
SDS-PAGE电泳结果显示提取纯化的Ⅱ型胶原蛋白与S igm a公司的产品一致。
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Extract total proteins from these two tissue samples respectively and then employ a SDS-PAGE method to identify the most differentially expressed protein between them and estimate the molecular weight and relative abundance ratio of the protein.
从这两个组织中分别提取总蛋白并定量,然后采用 SDS-PAGE 方法识别出在这两个组织中差异表达最为明显的蛋白质并且估计它的分子质量以及相应的丰度。
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This study evaluated the effect of SDS-PAGE with the low C separating alkalescence peptides under the different conditions.
本试验初步研究了低交联度的SDS-PAGE分析蜂毒碱性多肽的方法及有关条件的优化。
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Besides, the effect of acetone on fat and analysis of alkalescence peptides by SDS-PAGE were also evaluated in this paper.Acetone and Momordica charantia L.
同时,研究了丙酮对苦瓜种仁和蜜蜂毒素脂类物质的清除作用,以及碱性多肽的SDS-PAGE分离和分析方法。
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The molecular weight of amidase was estimated to be 60.6×103 by 12.5% SDS-PAGE.
用12.5% SDS-PAGE测得该酶的分子量约为60.6 kD。
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The molecular weight of amidase was estimated to be 60.6 kD by 12.5% SDS-PAGE.
用12.5% SDS-PAGE测得该酶的分子量约为60.6 kD。
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Page often scoots around on Rollerblades or rides a bike.
Page 则会经常踩着轮滑或者是脚踏车)两个人都是出了名的工作狂,不过华尔街也八卦了一番,报道说他们都分别有了女朋友,&根据公司的相关人士透露,Page先生已经在和一名Google的员工约会, Brin 先生也开始和公司里面一位员工的妹妹互相往来。&
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RESULTS: Four hybridoma cell lines secreting mAbs to PAGE 4 with high specificity and sensitivity were obtained.
PAGE是CT家族中的一族新分子,该家族包括5个成员,分别被命名为PAGE 1, 2, 3, 4和 5[3,4]。
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Dorsalis by salting out, ion exchange chromatography, and gel filtration chromatography. By salting out the egg homogenate, SDS-PAGE analysis revealed that the Vns were mainly precipitated in a 50~70% ammonium sulfate solution. The salted-out proteins were further purified by ion exchange chromatography, and the result showed that Vns were mainly eluted with a 0.3~0.5 M sodium chloride gradient. Finally, the elutes of ion exchange chromatography were refined by running them through gel filtration chromatography to obtain purified Vns. SDS-PAGE separated the 48- and 51-kDa purified Vns, and used them as antigens to raise polyclonal antibodies.
利用硫酸铵盐析法初步分离果实蝇卵中的蛋白质,以SDS-PAGE蛋白质电泳分析得知,卵黄蛋白主要在硫酸铵溶液浓度为50~70%时被沉淀出;盐析沉淀所得之卵黄蛋白,再以离子交换层析法进一步纯化显示,卵黄蛋白主要会在0.3~0.5 M氯化钠的浓度范围间被析出来;再将析出液浓缩后,经凝胶过滤层析法做最后的纯化,即获得纯化之卵黄蛋白。
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The total proteins from TGIF transfectant and vector control cells were separated by 2D electrophorosis. 760±41 and 680±38 spots were detected in TGIF transfectant and vector control cells respectively. Locus repeat analysis of protein spots showed that the mean deviation in IEF direction in TGIF transfectant and vector control cells were 0.864±0.123 mm and 0.843±0.115 mm respectively whereas the mean deviation in SDS-PAGE direction in these two cells were 0.812±0.109 mm and 1. 125±0.123 mm respectively. So the well-resolved and reproducible 2DE patterns from TGIF transfectant and vector control cells were established.
通过双向凝胶电泳分别分离TGIF转染细胞和PcDNA3.1空白质粒转染细胞的总蛋白质,测得两组细胞的蛋白质斑点数分别为760±41和680±38个;位置重复性分析表明TGIF转染细胞在等电聚焦方向上的平均偏差为0.864±0.123mm,在垂直板SDS-PAGE电泳方向上的平均位置偏差为0.812±0.109mm;PcDNA3.1空白质粒转染细胞在IEF方向上的平均偏差为0.843±0.115mm,在垂直板SDS-PAGE电泳方向上的平均位置偏差为1.125±0.123mm。
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- 推荐网络例句
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With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.
随着死亡的吉他手Schuldiner接受主唱的职务,乐队在现实中树立了重要的影响。
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But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.
不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐,因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。
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Closes the eye, the deep breathing, all no longer are the dreams as if......
关闭眼睛,深呼吸,一切不再是梦想,犹如。。。。。。