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In the sixth chapter, we develop the WISES, in detail Java language is used to compile the mail program, SQL is used to manage the data system and Frontpage is used to make web page.

其中,用Java语言编写了主体程序,用SQL语言管理数据系统,用Fro址Page制作了网页。

Native molecular mass of Vg in the Bombyx mandarina is around 440kD through Native-PAGE of egg substracts of the two silkworms.

通过Native-PAGE分析推定了野桑蚕卵黄原蛋白在天然状态下的分子量约为440kD。

Black rice prolamine s subunit compositions of 9 lines were studied with SDS—PAGE.

以9个品种的黑稻种子为材料,用SDS—PAGE方法,分析了各个品种的醇溶蛋白亚基组成。

Using A-PAGE (Acid-polyacrylamide gel electrophoresis), genetic diversity of prolamine was analyzed among 93 safflower accessions collected by Germplasm Resourse Information Network.

利用A-PAGE(Acid-polyacrylamide gel electrophoresis)技术对来源于不同国家的79份油用型红花材料醇溶蛋白位点进行检测。

It is featuring a pair of impressive oversized oculars with internal focusing, i.e. they neither move nor turn when focused. Fantao provides on his highly recommended Binofan web-page a detailed description of this sophisticated 7-lens eyepiece construction.

它的特征是令人印象深刻的过大的目镜,内部调焦,即调焦时不会转动和上下移动,Fantao在他的被高度推荐的 Binofan web-page 提供了这高级目镜的7镜片结构的详细描述(译者:连场镜也算成目镜了)。

Devise a pair of primer to put out the section of pgE which code the dense antigen episode, clone into pET-32a, name the masculine plasmid with pET-32pgE. Transform the BL21 strain with pET-32pgE, choose several single bacterium drops at random and contaminate 2X YT. vibrate at 37癈 over night. Contaminate another tube with the above culture to the final concentration of 2%, continue to culture to OD6oo0.6, add IPTG to the final concentration of Immol/L. inducing 6 hours. Analyse the expression with SDS-PAGE and Western-blot, the result is that the expression has actually happened and the product has immunoreactivity.

设计一对引物从gE全基因克隆载体pgE中调出抗原决定簇密集编码区,克隆进PET-32a表达载体,双酶切和PCR鉴定出阳性者,命名为PET-32pgE,转化BL21菌株,挑取单菌落在加有氨苄的2×YT培养基中37℃过夜长至饱和,按2%体积转种另一管,培养至OD_(600)为0.6,加IPTG至终浓度1mmol/l,诱导4~6小时,SDS-PAGE和Westernblot分析,证明确实表达并具有免疫反应性。

Results The recombinant plasmid pSNAV2.0-TK was verified byPCR and restricton endonuclease. The coat protein of AAV was detectedby SDS-PAGE method, the purity of rAAV2/HSV-TK was>98% byHPLC method and the titre of rAAV2/HSV-TK was 1×10~(12)v.g./ml byDNA dot blot hybridization.

结果:重组质粒pSNAV2.0-TK PCR扩增的片段大小和酶切鉴定所切下的片段与预计结果一致;SDS-PAGE法检测到三条特征性的AAV外壳蛋白条带,HPLC法检测rAAV2/HSV-TK的纯度>98%;用DNA斑点杂交方法检测rAAV2/HSV-TK的滴度为1×10~(12)v.g。

SDS-PAGE can be used as one of classification methods for cockroaches.

SDS-PAGE可作为蜚蠊分类的手段之一。

SDS-PAGE results indicated that there was a clear target band in Muts and Mut+ recombinant culture supernatant after 48 hours culture respectively .

实验结果表明,诱导表达48h后,Mut+和Muts重组子表达的产物在SDS-PAGE胶上都出现了清晰的目的带。

MALDI-TOF-MS, combined with high-resolution 2D-SDS-PAGE gel electrophoresis, offer microbiologists powerful tools for bacterial proteome research.

MALDI-TOF-MS结合高分辨率的二维SDS-PAGE可以分析10-12摩尔水平的蛋白,是细菌蛋白质组研究过程中必不可少的工具。

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I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像,后来回台湾,甚至去年在北京,居然也四处看见她的海报,才惊讶她的魅力之大。

Ah may dee:You are chinese living in a democratic country.

你是居住在民主国家的中国人吧。