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Methods: Differences of cellular and extracellular soluble protein expression between high cariogenic potential streptococcus mutans 593 clinical isolate and low cariogenic potential streptococcus mutans 18 clinical isolate were compared by SDS-PAGE.

采用SDS-PAGE技术，比较分离自高龋患者的变形链球菌593号菌株和无龋健康人的变形链球菌18号菌株的菌体和菌外可溶性蛋白表达的差异。

All Mut+and Muts colones were induced respectively with methanol to secrete interesting peptide in shake-flask cultures for four days. After dialysis and lyophilization, the supernate of cultures were identified by SDS-PAGE and Western blotting to find the genetically engineered Pichia pastoris which expressed interesting peptide.

在摇瓶中分别用甲醇诱导Mut~+和Mut~s型转化子表达蛋白4d，取培养产物冻干浓缩，进行SDS-PAGE和Western blotting，分析蛋白的含量及纯度，筛选出能特异表达目的蛋白的基因工程菌。

The technology of separation and purification of the interesting protein was studied, centrifuge the sample and get the clear supernatant, remove protein by 50％ ethanol, concentrate by drying with freezing way, remove salt and pigment by dialysing, separation with G-50 and CM-cellulose52 column, concentrate by PEG6000, The purified sample was a single band after separation by SDS-PAGE.

研究了目的阿片肽的分离纯化工艺。发酵混合物经过离心取上清，乙醇沉淀去蛋白，冷冻干燥浓缩，透析除盐脱色，聚乙二醇6000反渗透浓缩，G-50柱分离，CM-cellulose52阳离子交换层析，聚乙二醇6000浓缩等步骤后得到了较纯的目的产物，目的产物在SDS-PAGE上呈现单一条带。

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

The changes of biochemical properties were studied by extractability and ATPase activity measurement, SDS-PAGE analysis, and ultrastructure observation of fish meat from grass carp treated with different concentration TP and stored at 4℃.

研究方法及结果如下：1、通过测定盐溶性蛋白质的提取性、ATP酶活性、SDS-PAGE凝胶电泳、扫描电镜，分析4℃贮藏条件下的不同浓度TP处理的草鱼鱼肉蛋白质的生化特性的变化。

After the sequence and open reading frame were confirmed by DNA sequencing, the GADes gene was expressed in E.coli. SDS-PAGE analysis suggested that the bacteria BL21 produce a kind of fusion protein of 92KD and the expressed GADes fusion protein shows expected immunoreactivity by Western Blot.

SDS-PAGE电泳和Western Blot分别鉴定融合蛋白大小和免疫活性后，对表达条件进行优化，对包涵体进行分离、溶解和复性处理，以提高可溶性融合蛋白的产量，使融合蛋白达总蛋白的50％，相当于1.495g/每升培养物。

The gag included a mock press release quoting Google co-founder and president Larry Page, a step-by-step online installation manual, and a scatological selection of Frequently Asked Questions.

这一恶作剧包括一份&引用&Google创始人Larry Page的新闻稿，一份详细的安装的安装手册，还有一份搞笑的FAQ。

The gag included a mock press release quoting Google co-founder and president Larry Page, a step-by-step online installation manual, and a scatological selection of Frequently Asked Questions.

恶作剧包括Google共同创办人、总裁Larry Page在新闻中的发言、详细在线安装步骤和一段龌龊的&常见问题&摘录。

They began with a brisk version of "Good Times, Bad Times," with Page's guitar crisp and clear but Plant's voice cramped by feedback.

他们以轻快的&Good Times， Bad Times&开场，Page吉他弹奏的清脆干爽但是Plant的声音却被麦克的回声干扰的含混不清。

The deleted mutant PAP gene was also cloned into yeast secreted expression pPIC9K vector to form pPIC9K~3, then the vector was transferred into Pachia pastoris GS115 strain. The specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50-60 U g per millilitre measured by UV-absorbed methods in the supernatant of the medium via high density fermentation. SDS-PAGE results showed that there was one protein band in the gel which molecular weight was about 34Ku . The protein could specific react with France PAP antiserum in Western-blotting. Activity tests revealed t

将缺失型PAP基因克隆于酵母分泌型表达载体PPICgK构成重组载体，然后导入毕赤酵母（P8chia nastoris）菌株GSlls细胞中，在甲醇的诱导下，经过酵母高密度发酵进行PAP的表达，经SDS－PAGE分析，结果表明，在培养基上清液中含有一明显的特异性蛋臼条带，大小为34Ku，经Western－blotting分析，该蛋白与法国PAP抗血清有特异性反应，体外活性检测表明该蛋白对TMV的侵染性具有高度的抑制性，说明该 PAP基因在毕赤酵母 GS中也得到了正确表达。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。