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Here, Page spills his tips on how to ease into the trend.

在这里， Page 泄漏了如何缓和的融入这一趋势的方法。

The recombinant viruses, with the gene of reference RV strain VP2, field strain T114 VP6 and T73 G1-serotype VP7, respectively, co-infected Sf9 cell at a multiplicity of infection of 0.5. Harvested the supernatants of culture when the cells were lysed fully (about 7-10 days), or harvested cells before cell lysates (about 4-5 days after infection) and then treated the cells with lysing solution. The supernatants of and cell lysates were ultracentrifuged twice with 40% sucrose cushion at 93 000g, and the sediment products were harvested with TNC buffer. The purified VLPs samples were separated by SDS-PAGE, and then stained by Coomassie blue and silver nitrate and detected by Western blot to analyze the composition of VP2, VP6 and VP7 and their specificity. The morphologic structure of recombinant VLPs was detected by EM.

含标准株VP2、地方株T114 VP6和地方株T73 G1型VP7蛋白编码基因的重组病毒以0.5 MOI共感染Sf9细胞制备2／6／7病毒样颗粒，待细胞完全裂解后收获培养基上清或感染后5天细胞裂解前收获细胞并用裂解液裂解，培养基上清和细胞裂解液样品两次40％蔗糖垫93000g超速离心，沉淀样品经SDS-PAGE胶分离，用考马氏亮兰和硝酸银染色以及Western blot检测样品中VP2、VP6和VP7蛋白的组成以及特异性，电镜检测重组病毒样颗粒的形态结构。

So the improved Tris-HCl extraction was the most appropriate method for apple leaf SDS-PAGE analysis.

因此，改良的Tris-HCl法适用于苹果叶片的SDS-PAGE分析。

Results 61 mg IgY was extracted from one egg. The results of SDS-PAGE and Western blotting demonstrated that the IgY contained one major protein band with molecular weight of 130 000 and could be recognized by SEA.

结果 海兰母鸡经SEA首次免疫后35 d，每枚蛋经提纯后可得到约61 mg抗体，经SDS-PAGE和Western blotting分析，纯化的IgY有1条主要蛋白带，相对分子质量为130 000，并可被SEA识别。

This plas mid w as transformed into E.coli BL21 strain. The major band of about 31000 fusion prot ein could be detected by SDS-PAGE analysis and the fusion protein is more than 34% of total cellular protein.

该基因被克隆到融合型表达载体pGEX-2T中，重组融合型表达载体nCT/pGEX-2T转化E.coliBL21，培养表达，SDS-PAGE分析可见Mr约31000的融合蛋白带，融合蛋白占细胞内总蛋白的34%。

This paper study compose of coconut water protein by SDS-PAGE gel electrophoresis. The results showed that there were nine subunits in coconut water protein.

对椰子水中的蛋白质进行分离纯化，利用SDS-PAGE凝胶电泳系统，对椰子水中蛋白的结构进行了分析，结果表明，在椰子水蛋白质中，含有9个亚基结构。

Swine enteropathogenic E. colis were rejuvenescenced and 6 of them were selected to prepare blood serum. We had tested the reactance of cross agglutination and agar diffusion between the strains and the blood serums. No. 300 and No. 343 were selected because they had highly cross reaction. To test superantigen of No.300 and No.343, the antigens made by both of them immunized piglets, and then we detected the serum 0D values with indirect ELISA. The OMP and PF of No. 300 and No. 343 were extracted for SDS—PAGE and westen-blotting.

本研究针对10株猪致病性大肠杆菌，经复壮后选取致病性强的6菌株作为供试菌株，制备免疫血清与这6菌株的多种抗原分别进行交叉凝集试验和琼脂扩散试验，根据各菌株抗原的交叉反应程度，筛选出抗原交叉程度高的菌株300和343，检测两菌株超抗原的毒性作用，以不同方式制备抗原免疫仔猪，用间接ELISA检测免疫效果，并提取菌株300和343的外膜蛋白和渗透因子经SDS—PAGE及免疫印迹试验，进行相关分析。

Effects of Dabaryomyces hansenula on proteolysis during processing of dry-salted duck were studied with that with no bacterial as CK. SDS-PAGE was used to analyze proteolysis of dry-salted duck samples of different processing phases and the free-amino acids were tested as well.

以德汉逊氏酵母为发酵剂，研究其在风鸭加工过程中对肌肉蛋白降解的影响；通过SDS-PAGE电泳分析蛋白质的降解规律，比较加工初期与末期游离氨基酸含量的变化。

Prokaryotic expressed protein products of candidate cDNAs were purified by SDS-PAGE and used as immunogens to prepare murine antisera.

5利用SDS-PAGE分离原核表达产物，并以纯化的表达产物为免疫原免疫小鼠，制备抗血清。

Biles from an animal model of incomplete stenosis of common bile duct in guinea pigs and from patients with and without gallstone were examined for the total protein concentration, protein fractionations by SDS-PAGE electrophoresis, endogenous and exogenous .beta.-glucuronidase activity, bilirubin fractionations by HPLC, ionized calcium activity, pH, cholesterol and bile salts concentrations.

我们利用天竺鼠总胆管不完全结扎的动物模式之胆汁与患有胆结石病人之胆汁，进行下列测定：总蛋白质浓度、SDS-PAGE蛋白质电泳分析、内源性与外源性葡萄糖醛酸酵素活性、胆红素高效能液态层析仪分型浓度、游离钙活性、pH值、胆固醇浓度与胆汁酸浓度。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。