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1D-SDS-PAGE eletrophoresis and molecular markers were employed to survey the Waxy proteins and Waxy genes in 312 winter wheat cultivars and lines from Chinese winter wheat and U.S.A. and Austrilia, 60 lines selected from the cross of Baihuomai and Norin 67 which was detected preliminarily to be waxy wheat with Iodine-dyeing-seed-section , 120 F2 plants from cross of Lu935031(Jimai19) and Yumai 47 . Amylose content and RVA properties were also recorded. The major results were presented below:1 The components of waxy protein subunits could be detected precisely by 1D-SDS-PAG Eelectro- phoresis.

以国内外冬播小麦品种312份、从白火麦×Norin 67中选育出经碘染籽粒剖面初步鉴定为糯性小麦的品系60份以及鲁935031×豫麦47的F2代120个单株为材料，利用1D-SDS-PAGE电泳技术研究了Waxy蛋白亚基组成，并利用分子标记检测了相应Waxy基因；检测了部分材料的直链淀粉含量和RVA特性等，分析了国内主要麦区小麦淀粉特性；结合生化标记、分子标记和淀粉特性三者各自的优势，探讨了快速、准确、简便筛选具有优良淀粉品质的小麦资源和品种的途径。

Methods The type Ⅳ pilin protein PILE was purified by HisTrapTM HP affinity columns ,then the purified protein was examined by SDS-PAGE and Western blot.

使用HisTrapTM HP亲和层析柱纯化Ⅳ型菌毛蛋白PILE，并对纯化蛋白进行SDS-PAGE和Westernblot鉴定。

Bio-active α-hydroxynitrilelyase expressed in Hansenula polymorpha after 0.5% methanol induction was obtained with 2.015 U determined by analysis of enzyme activity and SDS-PAGE.

酶活测定和SDS-PAGE分析显示HNL在汉逊酵母NCYC495(leu1.1)中得到正确表达，每毫升发酵液中获得2.015 U的醇腈酶。

GFP antibodies were used to immunoprecipitate proteins that were subjected to SDS-PAGE and transferred to a polyvinylidene difluoride membrane, and the presence of HAX-1 was detected using specific antibodies.

24小时后，细胞裂解形容准备在&材料和方法&绿色荧光蛋白质的抗体来immunoprecipitate遭到SDS-PAGE和转让给聚氟膜而在场的HAX-1利用特异性抗体检测。

Twenty-four h later, cell lysates were prepared as described under "Materials and Methods." GFP antibodies were used to immunoprecipitate proteins that were subjected to SDS-PAGE and transferred to a polyvinylidene difluoride membrane, and the presence of HAX-1 was detected using specific antibodies.

24小时后，细胞裂解形容准备在&材料和方法&绿色荧光蛋白质的抗体来immunoprecipitate遭到SDS-PAGE和转让给聚氟膜而在场的HAX-1利用特异性抗体检测。

METHODS:The gene of human ET1 was synthesized according to the preferential codons of E. coli, cloned into the EcoRI and SalI sites of vector pThioHisA. The recombinant plasmid pThioHisA-ET1 was constructed , sequenced and transformed into E.coli TOP10. Induced and expressed fusion protein were identified and analysed by 12% SDS-PAGE and densitometry analyses. After the elution, denature and renature, the fusion protein Thioredoxin-ET1 was obtained by ProBondTM chromatogragraphy. The purity of Thioredoxin-ET1 was detected by HPLC. Inoculate Thioredoxin-ET1 once per mouse every 2 weeks in 25, 50 and 100μg separately on 3 groups for 4 times. 10 days after last inoculation, we obtained venipuncture blood.

根据人ET1的多肽序列合成ET1基因，将其插入到pThioHisA的EcoRI和 SalI位点，重组质粒pThioHisA-ET1进行酶切鉴定及序列测定验证后转化TOP10，IPTG诱导的重组菌经SDS-PAGE检测融合蛋白Thioredoxin-ET1的表达量；表达的融合蛋白用ProBond亲合层析纯化并经HPLC鉴测其纯度；每只小鼠按25、50、100ug/次剂量的Thioredoxin-ET1每两周免疫一次，共4次，最后一次免疫10d后制备抗血清，经Western blot和ELISA检测证明Thioredoxin-ET1融合蛋白具有ET1免疫反应原性。

To express chicken osteoprotegerin in Pichia pastoris and determine its inhibitive effects on mature osteoclastic bone resorptive function in vitro. The chOPG cDNA encoding for 382 amino acid without signal peptice was subcloned into Pichia pastoris expression vector pPICZα-A. The constructed plasmid was transformed into yeast X-33 by electroporation. The recombinant transformants were selected by Zeocin. Induced by the addition of methanol every 24 hours, the product analyzed by SDS-PAGE was sized about 43kDa and 53kDa at a yield of 200mg per litter of culture. The result of Western blotting indicated that the recombinant protein had specific antigenicity mainly owing to heterogeneous glycosylation.

为了在毕赤酵母中分泌表达鸡骨保护素（osteoprotegerin，OPG），采用DNA重组技术将chOPG成熟肽cDNA片段插入毕赤酵母表达载体pPICZα-A中，以电穿孔法转化酵母X-33，用Zeocin 平板筛选重组子，经甲醇诱导表达后， SDS-PAGE 和免疫印迹分析表达产物，由于糖基化不同，表达产物有两种，其相对分子量分别为43kDa和53kDa，表达量约为200mg/L，经Western印迹验证，有较好的抗原性。

The profiles of some silk proteins and isoenzymes were examined by SDS-PAGE. The results showed that there were no revealable changes in the structure proteins and other high expressive protein examined.

5用SDS-PAGE检测了几种丝蛋白质及其同工酶的表型，结果表明高压静电场处理没有引起检查的结构蛋白和其他高表达蛋白发生可检测到的变异。

Several methods of plant tissue protein extraction (TCA-acetone precipitation method, phenol method, urea method, Zhou Han-tao described method, and phenol method improved by the authors) were applied to extract total protein from Rhizophora stylosa root, and then after SDS-PAGE, a subsequent 2-DE analysis was carried out with the optimized conditions for 2-DE .

分别采用TCA-丙酮沉淀法、酚法、尿素法、周涵韬法和笔者建立的酚改良法，提取红海榄根部总蛋白质，并进行了SDS-PAGE分析和双向电泳体系的优化。

Coli. The outer membrane protein were extracted from 38 strains of avian E. coli which were isolated from the dead chickens of chicken breeding farms in 3 areas of Baoding, Qiuhuangdao and Beijing by using ultrasonic cleaving and N-Lauroyl Sarcosine Sodium, and OMP typing was done by SDS-PAGE.

方法］对分离自保定、秦皇岛和北京3个地区规模化养鸡场的病死鸡体内的38株大肠杆菌，采用超声波裂解和N-十二烷基肌胺酸钠进行外膜蛋白提取，利用SDS-PAGE进行Omp分型。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。