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By the analysis of SDS-PAGE electrophoresis, CXJZ95-198 secrets exocellular proteins bands in media of glucose, mannose ,xylan, mannosan and pectin respective were 32 bands, 33 bands,35 bands, 36 bands and 21 bands respectively .

在葡萄糖、甘露糖、木聚糖、魔芋粉及果胶五种不同碳源培养基中的发酵液在SDS-PAGE电泳中分别分泌出32条，33条，35条，36条和21条蛋白带；多数可比较的蛋白质电泳谱带的颜色深浅呈魔芋粉＞甘露糖＞木聚糖＞葡萄糖＞果胶的趋势。

The colour of most detected bands became light in the five media with the order of mannosan, mannose, xylan, glucose and pectin.(4)The pectinase in the supernatant was purified by centrifuge , acetone precipitation and sephadex G-75 cloumngel filtration. The specific activity was increased 9.8-fold with an activity recovery of 32.98%.The molecular weight of pectinase was 42.2KD estimated by SDS-PAGE.

确立了以葡萄糖培养基6h的发酵液为提取果胶酶的最初取样点，发酵液经离心，丙酮沉淀，Sephadex G-75葡聚糖凝胶柱层析后获得了凝胶电泳均一的样品，比活力较原酶液提高了9.8倍，回收率达到了32.98%，用SDS-PAGE凝胶电泳测的纯化后的果胶酶分子量为42.2KD。

The thesis studied the total exocellular proteins, reductant sugar and the pectinase enzyme activity of CXJZ95-198 strain in glucose, mannose, xylan, mannosan and pectin culture media.Compared enzyme activity of pectinase, xylanose and β-mannanose. The secreted proteins in ferment fluid from different culture media were analysed by SDS-PAGE electrophoresis.

本论文对CXJZ95-198菌株在葡萄糖、甘露糖、木聚糖、魔芋粉及果胶五种不同碳源培养基中胞外酶蛋白质总量、还原糖及果胶酶活性的影响进行了研究；对果胶酶、木聚糖酶及β-甘露聚糖酶在甘露糖培养基中的酶活进行了比较；对CXJZ95-198菌株在不同碳源培养基中的发酵液在SDS-PAGE电泳中分泌出的蛋白带作了比较；对CXJZ95-198菌株所产生的胞外酶系中果胶酶进行了分离纯化。

METHODS:Eighteen cases of meibomian gland carcinoma were resected ,and telomerase activity in these surgical specimens were measured by PCR-ELISA and PCR-PAGE.

选取18例睑板腺癌患者切除后的手术标本，采用PCR-ELISA法及PCR-PAGE法检测其端粒酶的含量及表达。

At the depigmentation assay, DH and EH show dose-dependently inhibitory activities against mushroom tyrosinase, and the IC50 is 5.697 and 5.256 mM, respectively, and show noncompetitively inhibitory modes in the present of tyrosine, but not DOPA. The control synthetic products, D and E, have no inhibitory effects on tyrosinase. Using tyrosinase activity staining in PAGE gels, both DH and EH show dose-dependently inhibitory pattern in the presence of tyrosine, while, EH also could suppress tyrosinase activity in the presence of DOPA.

而美白活性方面，以商品酪胺酸以商品酪胺酸酵素进行实验，当基质为酪胺酸时，发现DH与EH具浓度相关抑制效果，IC50分别为5.697与5.256 mM，且其相对於基质酪胺酸之抑制型态皆为非竞争型抑制，而其结构类似物之对照化合物D与E则不具此活性；但当基质为多巴时则皆不具此活性；利用原态PAGE电泳活性染色亦发现抑制酪胺酸酵素结果，但EH在基质为多巴时也能下降酪胺酸酵素活性。

Purified GO had a molecular weight between 145, 000 and 162, 300, as determined by Sephadex G-200 filtration and SDS-PAGE of the enzyme as cross-linked with glutaraldehyde. This result showed that the holoenzyme was a tetramer, and no change occurred in molecular weight of the enzyme during the s〓orage. The active form of GO from brassica leaves showed a constant molecular weight during purification, so did that from O. sativa leaves during greening of etiolated seedlings, maybe acting as a stable tetramer.

用Sephadex G-200胶过滤和戊二醛交联后的SDS-PAGE法测得纯化的菜心叶片GO的分子量在145，000-162，300间，为四聚体；并且在放置过程中其分子量和聚合态均保持不变，为稳定的四聚体；进一步研究表明菜心叶片GO在纯化过程中和水稻叶片GO在黄化苗的转绿过程中，两种GO活性状态的分子量和聚合态也没有发生变化，均呈四聚体。

The technique of PAGE gel analysis has been widely used in separation of low molecular weight PCR product. Due to the overmany technical steps and large time consumption, this technique is disadvantageous to carrying out molecular marker analysis in large scale.

PAGE凝胶分析技术已广泛运用于小分子量PCR产物的分离，但因操作环节多、耗时长，不利于大规模分子标记分析工作的开展。

The antigen trait and immunogenicity of E. ovi were analyzed using SDS-PAGE and western blotting, then the E. ovis antigen was used as a diagnostic antigen to detect 64 serum samples by ELISA.

将提取的羊附红细胞体抗原进行SDS-PAGE电泳及免疫印迹分析，并以此抗原为诊断杭原，用ELISA方法对64份羊的血清样本进行了初步检测。

The changes of soluble protein composition during the pre-stage, early stage and advanced stage of cantharidin synthesis in M. calida Palla were analysed by SDS-PAGE and two-dimensional electrophoresis.

本研究采用 SDS-PAGE 、双向电泳技术，分析了苹斑芫菁 Mylabris calida Palla 在斑蝥素合成前期、大量合成早期和末期的蛋白质组成的变化。

But SDS-PAGE and immunodetection indicated that both mutants can not secrete into the periplasm like the wide type protein.

但SDS-PAGE和Western-blot鉴定表明突变体不再能象野生型一样分泌到胞外的间质中。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。